HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shao, H. Articles by Sun, D. Search for Related Content PubMed PubMed Citation Articles by Shao, H. Articles by Sun, D.

A Shared Epitope of the Interphotoreceptor Retinoid-Binding Protein Recognized by the CD4⁺ and CD8⁺ Autoreactive T Cells¹

Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY 40202

We previously demonstrated that cultures of rat uveitogenic T cells rapidly become dominated by CD4⁺ cells, but activation of CD8⁺ autoreactive T cells also occurred during the in vitro culture of in vivo-primed T cells. In the present study, we show that the commonly used uveitogenic peptide, interphotoreceptor retinoid-binding protein (IRBP) 1–20, generated both CD4⁺ and CD8⁺ autoreactive T cells in the C57BL/6 (B6) mouse and that this 20-mer contains at least two distinct antigenic epitopes. To determine whether the CD8 response was Ag-specific and whether CD4⁺ and CD8⁺ IRBP1–20-specific T cells recognize distinct antigenic epitopes, we prepared highly purified CD4⁺ and CD8⁺ T cells from IRBP1–20-primed mice and tested their proliferative response to a large panel of truncated peptides derived from IRBP1–20. The results showed that both CD4⁺ and CD8⁺ T cells recognized the same spectrum of peptides. In addition, peptides P10–18 were found to bind effectively to CD8⁺ IRBP1–20-specific T cells when complexed with recombinant H-2K^b and also stimulate the proliferation and cytokine production of CD4⁺ IRBP1–20-specific T cells. Our results document for the first time that CD8⁺ and CD4⁺ autoreactive T cells display characteristic epitope recognition and they both recognize the same core epitope.

This article has been cited by other articles:

				Y. Peng, G. Han, H. Shao, Y. Wang, H. J. Kaplan, and D. Sun Characterization of IL-17+ Interphotoreceptor Retinoid-Binding Protein-Specific T Cells in Experimental Autoimmune Uveitis Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4153 - 4161. [Abstract] [Full Text] [PDF]

				Y. Ke, D. Sun, P. Zhang, G. Jiang, H. J. Kaplan, and H. Shao Suppression of Established Experimental Autoimmune Uveitis by Anti-LFA-1{alpha} Ab Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2667 - 2675. [Abstract] [Full Text] [PDF]

				Y. Peng, H. Shao, Y. Ke, P. Zhang, G. Han, H. J. Kaplan, and D. Sun Minimally Activated CD8 Autoreactive T Cells Specific for IRBP Express a High Level of Foxp3 and Are Functionally Suppressive Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2178 - 2184. [Abstract] [Full Text] [PDF]

				P. Zhang, D. Sun, Y. Ke, H. J. Kaplan, and H. Shao The Net Effect of Costimulatory Blockers Is Dependent on the Subset and Activation Status of the Autoreactive T Cells J. Immunol., January 1, 2007; 178(1): 474 - 479. [Abstract] [Full Text] [PDF]

				Q. Ouyang, N. E. Standifer, H. Qin, P. Gottlieb, C. B. Verchere, G. T. Nepom, R. Tan, and C. Panagiotopoulos Recognition of HLA Class I-Restricted {beta}-Cell Epitopes in Type 1 Diabetes Diabetes, November 1, 2006; 55(11): 3068 - 3074. [Abstract] [Full Text] [PDF]

				Y. Peng, H. Shao, Y. Ke, P. Zhang, J. Xiang, H. J. Kaplan, and D. Sun In Vitro Activation of CD8 Interphotoreceptor Retinoid-Binding Protein-Specific T Cells Requires not only Antigenic Stimulation but also Exogenous Growth Factors. J. Immunol., April 15, 2006; 176(8): 5006 - 5014. [Abstract] [Full Text] [PDF]