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CD43 Is Required for Optimal Growth Inhibition of Mycobacterium tuberculosis in Macrophages and in Mice¹

April K. Randhawa^*,¶, Hermann J. Ziltener^,, Jasmeen S. Merzaban^*, and Richard W. Stokes^2,*,,,¶

^* Department of Medicine, Department of Pathology and Laboratory Medicine, Department of Paediatrics, and Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada, University of British Columbia; and ^¶ Division of Infectious and Immunological Diseases, British Columbia’s Children’s and Women’s Hospital, Vancouver, British Columbia, Canada

We explored the role of macrophage (M) CD43, a transmembrane glycoprotein, in the pathogenesis of Mycobacterium tuberculosis. Using gene-deleted mice (CD43^–/–), we assessed the association of the bacterium with distinct populations of M and found that CD43^–/– M bound less M. tuberculosis than CD43^+/+ M. Increased infective doses did not abrogate this difference. However, reduced association due to the absence of CD43 could be overcome by serum components. M from heterozygote mice, which express 50% of wild-type CD43, bound more bacteria than CD43^–/– but less than CD43^+/+, proving that the gene dose of CD43 correlates with binding of M. tuberculosis. Furthermore, the reduced ability of CD43^–/– M to bind bacteria was restricted to mycobacterial species. We also found that the survival and replication of M. tuberculosis within M was enhanced significantly in the absence of CD43, making this the first demonstration that the mechanism of mycobacterial entry influences its subsequent growth. Most importantly, we show here that the absence of CD43 in mice aerogenically infected with M. tuberculosis results in an increased bacterial load during both the acute and chronic stages of infection and more rapid development of granulomas, with greater lung involvement and distinctive cellularity.

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