| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
, but Not MyD88, Regulate Escherichia coli-Induced Dendritic Cell Maturation and Apoptosis In Vivo1
* Laboratory of Animal Physiology, Institut de Biologie et de Médecine Moléculaire, Université Libre de Bruxelles, Gosselies, Belgium; Laboratory of Immunology of Infectious, Allergic and Autoimmune Diseases, Institut National de la Santé et de la Recherche Médicale, Institut de Pharmacologie Moleculaire et Cellulaire, Université de Nice-Sophia Antipolis, Valbonne, France; and Laboratoire de Parasitologie, Faculté de Médecine, Université Libre de Bruxelles, Brussels, Belgium
Dendritic cells (DC) are short-lived, professional APCs that play a central role in the generation of adaptive immune responses. Induction of efficient immune responses is dependent on how long DCs survive in the host. Therefore, the regulation of DC apoptosis in vivo during infection remains an important question that requires further investigation. The impact of Escherichia coli bacteremia on DCs has never been analyzed. We show here that i.v. or i.p. administration of live or heat-killed E. coli in mice induces splenic DC migration, maturation, and apoptosis. We further characterize which TLR and Toll-IL-1R (TIR)-containing adaptor molecules regulate these processes in vivo. In this model, DC maturation is impaired in TLR2–/–, TLR4–/– and TIR domain-containing adapter-inducing IFN-
(TRIF)–/– mice. In contrast, DC apoptosis is reduced only in TLR4–/– and TRIF–/– mice. As expected, DC apoptosis induced by the TLR4 ligand LPS is also abolished in these mice. Injection of the TLR9 ligand CpG-oligodeoxynucleotide (synthetic bacterial DNA) induces DC migration and maturation, but only modest DC apoptosis when compared with LPS and E. coli. Together, these results suggest that E. coli bacteremia directly impacts on DC maturation and survival in vivo through a TLR4-TRIF-dependent signaling pathway.
This article has been cited by other articles:
|
|
 |
|
  F. Pene, E. Courtine, F. Ouaaz, B. Zuber, B. Sauneuf, G. Sirgo, C. Rousseau, J. Toubiana, V. Balloy, M. Chignard, et al. Toll-Like Receptors 2 and 4 Contribute to Sepsis-Induced Depletion of Spleen Dendritic Cells Infect. Immun., December 1, 2009; 77(12): 5651 - 5658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Alzabin, N. Bhardwaj, F. Kiefer, S. Sawasdikosol, and S. Burakoff Hematopoietic Progenitor Kinase 1 Is a Negative Regulator of Dendritic Cell Activation J. Immunol., May 15, 2009; 182(10): 6187 - 6194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sundquist and M. J. Wick Salmonella induces death of CD8{alpha}+ dendritic cells but not CD11cintCD11b+ inflammatory cells in vivo via MyD88 and TNFR1 J. Leukoc. Biol., February 1, 2009; 85(2): 225 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L Kao, W. Browder, and C. Li Cellular Cardiomyoplasty: What Have We Learned? Asian Cardiovasc Thorac Ann, January 1, 2009; 17(1): 89 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, K.-H. Park-Min, A. Yarilina, and L. B. Ivashkiv Regulation of STAT pathways and IRF1 during human dendritic cell maturation by TNF-{alpha} and PGE2 J. Leukoc. Biol., November 1, 2008; 84(5): 1353 - 1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bhushan, S. Tchatalbachev, J. Klug, M. Fijak, C. Pineau, T. Chakraborty, and A. Meinhardt Uropathogenic Escherichia coli Block MyD88-Dependent and Activate MyD88-Independent Signaling Pathways in Rat Testicular Cells J. Immunol., April 15, 2008; 180(8): 5537 - 5547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Peck-Palmer, J. Unsinger, K. C. Chang, C. G. Davis, J. E. McDunn, and R. S. Hotchkiss Deletion of MyD88 markedly attenuates sepsis-induced T and B lymphocyte apoptosis but worsens survival J. Leukoc. Biol., April 1, 2008; 83(4): 1009 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Macedo, D. M. Magnani, N. B. Carvalho, O. Bruna-Romero, R. T. Gazzinelli, and S. C. Oliveira Central Role of MyD88-Dependent Dendritic Cell Maturation and Proinflammatory Cytokine Production to Control Brucella abortus Infection J. Immunol., January 15, 2008; 180(2): 1080 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ghose, D. White, T. Guo, J. Vallejo, and S. J. Karpen Regulation of Hepatic Drug-Metabolizing Enzyme Genes by Toll-Like Receptor 4 Signaling Is Independent of Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein Drug Metab. Dispos., January 1, 2008; 36(1): 95 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Nolte, S. LeibundGut-Landmann, O. Joffre, and C. R. e Sousa Dendritic cell quiescence during systemic inflammation driven by LPS stimulation of radioresistant cells in vivo J. Exp. Med., June 11, 2007; 204(6): 1487 - 1501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. A. Hasan, C. Caux, I. Perrot, A.-C. Doffin, C. Menetrier-Caux, G. Trinchieri, M. Tommasino, and J. Vlach Cell proliferation and survival induced by Toll-like receptors is antagonized by type I IFNs PNAS, May 8, 2007; 104(19): 8047 - 8052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Power, B. Li, M. Yamamoto, S. Akira, and T.-J. Lin A Role of Toll-IL-1 Receptor Domain-Containing Adaptor-Inducing IFN-beta in the Host Response to Pseudomonas aeruginosa Lung Infection in Mice J. Immunol., March 1, 2007; 178(5): 3170 - 3176. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
