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Analysis of the Underlying Cellular Mechanisms of Anti-CD154-Induced Graft Tolerance: The Interplay of Clonal Anergy and Immune Regulation¹

Sergio A. Quezada^*, Kathy Bennett^*, Bruce R. Blazar, Alexander Y. Rudensky, Shimon Sakaguchi and Randolph J. Noelle^2,*

^* Department of Microbiology and Immunology, Dartmouth Medical School, and Norris Cotton Cancer Center, Lebanon, NH 03756; Division of Bone Marrow Transplantation, University of Minnesota, Minneapolis, MN 55455; Howard Hughes Institute, University of Washington, Seattle, WA 98195; and Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto, Japan

Although it has been shown that CD4⁺CD25⁺ regulatory T cells (T^reg) contribute to long-term graft acceptance, their impact on the effector compartment and the mechanism by which they exert suppression in vivo remain unresolved. Using a CD4⁺ TCR transgenic model for graft tolerance, we have unveiled the independent contributions of anergy and active suppression to the fate of immune and tolerant alloreactive T cells in vivo. First, it is shown that anti-CD154-induced tolerance resulted in the abortive expansion of the alloreactive, effector T cell pool. Second, commensurate with reduced expansion, there was a loss of cytokine production, activation marker expression, and absence of memory T cell markers. All these parameters defined the tolerant alloreactive T cells and correlated with the inability to mediate graft rejection. Third, the tolerant alloreactive T cell phenotype that is induced by CD154 was reversed by the in vivo depletion of T^reg. Reversal of the tolerant phenotype was followed by rapid rejection of the allograft. Fourth, in addition to T^reg depletion, costimulation of the tolerant alloreactive T cells or activation of the APC compartment also reverted alloreactive T cell tolerance and restored an activated phenotype. Finally, it is shown that the suppression is long-lived, and in the absence of anti-CD154 and donor-specific transfusion, these T^reg can chronically suppress effector cell responses, allowing long-lived graft acceptance.

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The JI 2005 175: 631-632. [Full Text]  

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