| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Divisions of*
Immunoregulation, Immune Cell Biology and Molecular Immunology, National Institute for Medical Research, London, United Kingdom; and CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115
The molecular mechanisms that regulate expression of the immunosuppressive cytokine IL-10 remain poorly understood. In this study, by measuring sensitivity to DNase I digestion, we show that production of IL-10 by primary mouse bone marrow-derived macrophages stimulated through pattern recognition receptors was associated with chromatin remodeling of the IL-10 locus. We also demonstrate that the IL-10 locus is remodeled in primary Th2 cells and IL-10-producing regulatory T cells that have been differentiated in vitro. Strikingly, a novel DNase I-hypersensitive site (HSS-4.5) was identified in stimulated macrophages, but not in T cells. We show that hyperacetylated histones were recruited to this site in stimulated macrophages. Furthermore, HSS-4.5 is highly conserved and contains a putative NF-
B binding site. In support of a function for this site, NF-B p65/RelA was recruited to HSS-4.5 in vivo and its activation was required for optimal IL-10 gene expression in LPS-stimulated macrophages.
This article has been cited by other articles:
|
|
 |
|
  F. Kaiser, D. Cook, S. Papoutsopoulou, R. Rajsbaum, X. Wu, H.-T. Yang, S. Grant, P. Ricciardi-Castagnoli, P. N. Tsichlis, S. C. Ley, et al. TPL-2 negatively regulates interferon-{beta} production in macrophages and myeloid dendritic cells J. Exp. Med., August 31, 2009; 206(9): 1863 - 1871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsuji-Takayama, M. Suzuki, M. Yamamoto, A. Harashima, A. Okochi, T. Otani, T. Inoue, A. Sugimoto, T. Toraya, M. Takeuchi, et al. The Production of IL-10 by Human Regulatory T Cells Is Enhanced by IL-2 through a STAT5-Responsive Intronic Enhancer in the IL-10 Locus J. Immunol., September 15, 2008; 181(6): 3897 - 3905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kube, T.-D. Hua, F. von Bonin, N. Schoof, S. Zeynalova, M. Kloss, D. Gocht, B. Potthoff, M. Tzvetkov, J. Brockmoller, et al. Effect of Interleukin-10 Gene Polymorphisms on Clinical Outcome of Patients with Aggressive Non-Hodgkin's Lymphoma: An Exploratory Study Clin. Cancer Res., June 15, 2008; 14(12): 3777 - 3784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dong, C. Ivascu, H.-D. Chang, P. Wu, R. Angeli, L. Maggi, F. Eckhardt, L. Tykocinski, C. Haefliger, B. Mowes, et al. IL-10 Is Excluded from the Functional Cytokine Memory of Human CD4+ Memory T Lymphocytes J. Immunol., August 15, 2007; 179(4): 2389 - 2396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Trinchieri Interleukin-10 production by effector T cells: Th1 cells show self control J. Exp. Med., February 19, 2007; 204(2): 239 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Flores, K. A. Diggs, L. M. Tait, and P. A. Morel IFN-{gamma} Negatively Regulates CpG-Induced IL-10 in Bone Marrow-Derived Dendritic Cells J. Immunol., January 1, 2007; 178(1): 211 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Schmidt, V. T. Thieu, B. A. Mann, A.-N. N. Ahyi, and M. H. Kaplan Bruton's Tyrosine Kinase Is Required for TLR-Induced IL-10 Production J. Immunol., November 15, 2006; 177(10): 7203 - 7210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Calado, T. Paixao, D. Holmberg, and M. Haury Stochastic Monoallelic Expression of IL-10 in T Cells J. Immunol., October 15, 2006; 177(8): 5358 - 5364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cao, X. Zhang, J. P. Edwards, and D. M. Mosser NF-{kappa}B1 (p50) Homodimers Differentially Regulate Pro- and Anti-inflammatory Cytokines in Macrophages J. Biol. Chem., September 8, 2006; 281(36): 26041 - 26050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shoemaker, M. Saraiva, and A. O'Garra GATA-3 Directly Remodels the IL-10 Locus Independently of IL-4 in CD4+ T Cells J. Immunol., March 15, 2006; 176(6): 3470 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Banerjee, R. Gugasyan, M. McMahon, and S. Gerondakis Diverse Toll-like receptors utilize Tpl2 to activate extracellular signal-regulated kinase (ERK) in hemopoietic cells PNAS, February 28, 2006; 103(9): 3274 - 3279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Jones and R. A. Flavell Distal Enhancer Elements Transcribe Intergenic RNA in the IL-10 Family Gene Cluster J. Immunol., December 1, 2005; 175(11): 7437 - 7446. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
