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Saliva from Lutzomyia longipalpis Induces CC Chemokine Ligand 2/Monocyte Chemoattractant Protein-1 Expression and Macrophage Recruitment¹

Clarissa R. Teixeira^*,, Maria Jânia Teixeira^*,, Regis B. B. Gomes^*,, Claire S. Santos^*, Bruno B. Andrade^*,, Imbroinise Raffaele-Netto^*, João S. Silva, Angelo Guglielmotti, José C. Miranda^*, Aldina Barral^*,,¶, Cláudia Brodskyn^*,¶ and Manoel Barral-Netto^2,*,,¶

^* Centro de Pesquisas Gonçalo Moniz-Fundação Oswaldo Cruz (CPqGM/FIOCRUZ), and Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brasil; Department of Pharmacology, Angelini Farmaceutici, Santa Palomba-Pomezia, Rome, Italy; Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil; and ^¶ Instituto do Milênio-CPqGM, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

Saliva of bloodfeeding arthropods has been incriminated in facilitating the establishment of parasite in their host. We report on the leukocyte chemoattractive effect of salivary gland homogenate (SGH) from Lutzomyia longipalpis on saliva-induced inflammation in an air pouch model. SGH (0.5 pair/animal) was inoculated in the air pouch formed in the back of BALB/c or C57BL/6 mice. L. longipalpis SGH induced a significant influx of macrophages in BALB/c but not in C57BL/6 mice. SGH-induced cell recruitment reached a peak at 12 h after inoculation and was higher than that induced by the LPS control. This differential cell recruitment in BALB/c mice was directly correlated to an increase in CCL2/MCP-1 expression in the air pouch lining tissue. In fact, treatment with bindarit, an inhibitor of CCL2/MCP-1 synthesis, and also with a specific anti-MCP-1 mAb resulted in drastic reduction of macrophage recruitment and inhibition of CCL2/MCP-1 expression in the lining tissue. CCL2/MCP-1 production was also seen in vitro when J774 murine macrophages were exposed to L. longipalpis SGH. The SGH effect was abrogated by preincubation with serum containing anti-SGH IgG Abs as well as in mice previously sensitized with L. longipalpis bites. Interestingly, the combination of SGH with Leishmania chagasi induced an increased recruitment of neutrophils and macrophages when compared with L. chagasi alone. Taken together these results suggest that SGH not only induces the recruitment of a greater number of macrophages by enhancing CCL2/MCP-1 production but also synergizes with L. chagasi to recruit more inflammatory cells to the site of inoculation.

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