The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, B.
Right arrow Articles by Liu, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, B.
Right arrow Articles by Liu, M.
The Journal of Immunology, 2005, 175: 8303-8311.
Copyright © 2005 by The American Association of Immunologists

TNF{alpha}-Induced Long Pentraxin PTX3 Expression in Human Lung Epithelial Cells via JNK

Bing Han*, Marco Mura*, Cristiano F. Andrade*, Daisuke Okutani*, Monika Lodyga*, Claudia C. dos Santos*, Shaf Keshavjee*, Michael Matthay{dagger} and Mingyao Liu1,*

* Thoracic Surgery Research Laboratory, University Health Network Toronto General Research Institute, Department of Surgery, University of Toronto, Toronto, Ontario, Canada; and {dagger} Critical Care Medicine, University of California, San Francisco, CA 94143

Long pentraxin 3 (PTX3), an acute-phase protein, is a newly clarified mediator for innate immunity and inflammation. As a soluble pattern recognition receptor, it has a nonredundant role in antifungal infection. Overexpression of PTX3 worsens acute lung injury. The lung epithelium is a critical factor in defense against pulmonary pathogens; it is also involved in acute inflammatory responses related to tissue injury. However, very little is known about how PTX3 is regulated in the lung epithelium. In this study, we found that i.v. injection of LPS induced PTX3 expression in rat lung alveolar epithelium. Using human lung cell lines and primary epithelial cells, we found that PTX3 expression was significantly up-regulated by TNF-{alpha} in a time- and dose-dependent manner, but not by LPS. Pretreatment with either actinomycin D or cycloheximide abolished TNF-{alpha}-induced PTX3 expression, indicating the requirement for both transcriptional and translational regulation. The TNF-{alpha}-induced PTX3 expression was blocked by SP600125, a JNK-specific inhibitor, but not by the inhibitors against NF-{kappa}B, ERKs, or p38 MAPK. Knockdown of either JNK1 or JNK2 with small interfering RNA also significantly reduced the regulated PTX3 expression. Thus, lung epithelial cells appear to be a major local source for PTX3 production, which could be induced in vivo from these cells by LPS or other inflammatory stimuli, and may be an important mediator for host defense and tissue damage. The importance of the JNK pathway for the regulated PTX3 expression may be a potential target for its regulation in the lung.




This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
S. J. Park and B. Mehrad
Innate Immunity to Aspergillus Species
Clin. Microbiol. Rev., October 1, 2009; 22(4): 535 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol Ind HealthHome page
J. D Thrasher and S. Crawley
The biocontaminants and complexity of damp indoor spaces: more than what meets the eyes
Toxicology and Industrial Health, October 1, 2009; 25(9-10): 583 - 615.
[Abstract] [PDF]

Home page
 Ann Rheum DisHome page
C Wright, M Edelmann, K diGleria, S Kollnberger, H Kramer, S McGowan, K McHugh, S Taylor, B Kessler, and P Bowness
Ankylosing spondylitis monocytes show upregulation of proteins involved in inflammation and the ubiquitin proteasome pathway
Ann Rheum Dis, October 1, 2009; 68(10): 1626 - 1632.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
Y.-C. Wu, P.-K. Hsu, K.-C. Su, L.-Y. Liu, C.-C. Tsai, S.-H. Tsai, W.-H. Hsu, Y.-C. Lee, and D.-W. Perng
Bile Acid Aspiration in Suspected Ventilator-Associated Pneumonia
Chest, July 1, 2009; 136(1): 118 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. G. Souza, F. A. Amaral, C. T. Fagundes, F. M. Coelho, R. M.E. Arantes, L. P. Sousa, M. M. Matzuk, C. Garlanda, A. Mantovani, A. A. Dias, et al.
The Long Pentraxin PTX3 Is Crucial for Tissue Inflammation after Intestinal Ischemia and Reperfusion in Mice
Am. J. Pathol., April 1, 2009; 174(4): 1309 - 1318.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Deban, H. Jarva, M. J. Lehtinen, B. Bottazzi, A. Bastone, A. Doni, T. S. Jokiranta, A. Mantovani, and S. Meri
Binding of the Long Pentraxin PTX3 to Factor H: Interacting Domains and Function in the Regulation of Complement Activation
J. Immunol., December 15, 2008; 181(12): 8433 - 8440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Doni, G. Mantovani, C. Porta, J. Tuckermann, H. M. Reichardt, A. Kleiman, M. Sironi, L. Rubino, F. Pasqualini, M. Nebuloni, et al.
Cell-specific Regulation of PTX3 by Glucocorticoid Hormones in Hematopoietic and Nonhematopoietic Cells
J. Biol. Chem., October 31, 2008; 283(44): 29983 - 29992.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. S. Tang, M. Mura, R. Seth, and M. Liu
Acute lung injury and cell death: how many ways can cells die?
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L632 - L641.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. Shaykhiev and R. Bals
Interactions between epithelial cells and leukocytes in immunity and tissue homeostasis
J. Leukoc. Biol., July 1, 2007; 82(1): 1 - 15.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Xu, X.-H. Bai, M. Lodyga, B. Han, H. Xiao, S. Keshavjee, J. Hu, H. Zhang, B. B. Yang, and M. Liu
XB130, a Novel Adaptor Protein for Signal Transduction
J. Biol. Chem., June 1, 2007; 282(22): 16401 - 16412.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. He, B. Han, and M. Liu
Long pentraxin 3 in pulmonary infection and acute lung injury
Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1039 - L1049.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Jaillon, G. Peri, Y. Delneste, I. Fremaux, A. Doni, F. Moalli, C. Garlanda, L. Romani, H. Gascan, S. Bellocchio, et al.
The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps
J. Exp. Med., April 16, 2007; 204(4): 793 - 804.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Okutani, B. Han, M. Mura, T. K. Waddell, S. Keshavjee, and M. Liu
High-volume ventilation induces pentraxin 3 expression in multiple acute lung injury models in rats
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L144 - L153.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Bozza, F. Bistoni, R. Gaziano, L. Pitzurra, T. Zelante, P. Bonifazi, K. Perruccio, S. Bellocchio, M. Neri, A. M. Iorio, et al.
Pentraxin 3 protects from MCMV infection and reactivation through TLR sensing pathways leading to IRF3 activation
Blood, November 15, 2006; 108(10): 3387 - 3396.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Okutani, M. Lodyga, B. Han, and M. Liu
Src protein tyrosine kinase family and acute inflammatory responses
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L129 - L141.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.