The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, H.
Right arrow Articles by Liversidge, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, H.
Right arrow Articles by Liversidge, J.
The Journal of Immunology, 2005, 175: 6915-6923.
Copyright © 2005 by The American Association of Immunologists

Differentiation to the CCR2+ Inflammatory Phenotype In Vivo Is a Constitutive, Time-Limited Property of Blood Monocytes and Is Independent of Local Inflammatory Mediators1

Heping Xu2,*, Ayyakkannu Manivannan{dagger}, Rosemary Dawson*, Isabel J. Crane*, Matthias Mack{ddagger}, Peter Sharp{dagger} and Janet Liversidge2,*

* Department of Ophthalmology, Institute of Medical Science, {dagger} Department of Bioengineering and Biophysics, University of Aberdeen, Aberdeen, United Kingdom; and {ddagger} Department of Internal Medicine II, University of Regensburg, Regensburg, Germany

It is proposed that CCR2+ monocytes are specifically recruited to inflammatory sites, whereas CCR2– monocytes are recruited to normal tissue to become resident macrophages. Whether these subsets represent separate lineages, how differential trafficking is regulated and whether monocytes undergo further differentiation is uncertain. Using a mouse model of autoimmune uveoretinitis we examined monocyte trafficking to the inflamed retina in vivo. We show that bone marrow-derived CD11b+ F4/80– monocytes require 24 to 48 h within the circulation and lymphoid system before acquiring the CCR2+ phenotype and trafficking to the inflamed retina is enabled. This phenotype, and the capacity to traffic were lost by 72 h. Monocyte CCR2 expression followed a similar time course in normal mice indicating that differentiation to an inflammatory phenotype is a constitutive, time-limited property, independent of local inflammatory mediators. Phenotypic analysis of adoptively transferred cells indicated that circulating inflammatory monocytes also differentiate into CD11c+ and B220+ dendritic cells and F4/80+ tissue macrophages in vivo. Our data supports the hypothesis of continuous extravasation and progressive differentiation over time of inflammatory monocytes in the circulation rather than replication within the actively inflamed tissue, and supports the concept of myeloid dendritic cell differentiation from trafficking monocytes under physiological conditions in vivo.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
M. P. Holt, L. Cheng, and C. Ju
Identification and characterization of infiltrating macrophages in acetaminophen-induced liver injury
J. Leukoc. Biol., December 1, 2008; 84(6): 1410 - 1421.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Shi, A. Maminishkis, T. Banzon, S. Jalickee, R. Li, J. Hammer, and S. S. Miller
Control of Chemokine Gradients by the Retinal Pigment Epithelium
Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4620 - 4630.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Xu, A. Manivannan, I. Crane, R. Dawson, and J. Liversidge
Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation
Blood, August 15, 2008; 112(4): 1166 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Wang, Y. Wang, Q. Cai, G. Zheng, V. W.S. Lee, D. Zheng, X. Li, T. K. Tan, and D. C.H. Harris
By Homing to the Kidney, Activated Macrophages Potently Exacerbate Renal Injury
Am. J. Pathol., June 1, 2008; 172(6): 1491 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Jakubzick, F. Tacke, F. Ginhoux, A. J. Wagers, N. van Rooijen, M. Mack, M. Merad, and G. J. Randolph
Blood Monocyte Subsets Differentially Give Rise to CD103+ and CD103- Pulmonary Dendritic Cell Populations
J. Immunol., March 1, 2008; 180(5): 3019 - 3027.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. P. Phadke, G. Akangire, S. J. Park, S. A. Lira, and B. Mehrad
The Role of CC Chemokine Receptor 6 in Host Defense in a Model of Invasive Pulmonary Aspergillosis
Am. J. Respir. Crit. Care Med., June 1, 2007; 175(11): 1165 - 1172.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. N. Lumeng, S. M. DeYoung, J. L. Bodzin, and A. R. Saltiel
Increased Inflammatory Properties of Adipose Tissue Macrophages Recruited During Diet-Induced Obesity
Diabetes, January 1, 2007; 56(1): 16 - 23.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. Rasmussen, J. Cello, H. Gil, C. A. Forestal, M. B. Furie, D. G. Thanassi, and J. L. Benach
Mac-1+ Cells Are the Predominant Subset in the Early Hepatic Lesions of Mice Infected with Francisella tularensis
Infect. Immun., December 1, 2006; 74(12): 6590 - 6598.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. E. Qualls, A. M. Kaplan, N. van Rooijen, and D. A. Cohen
Suppression of experimental colitis by intestinal mononuclear phagocytes
J. Leukoc. Biol., October 1, 2006; 80(4): 802 - 815.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.