The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shurin, G. V.
Right arrow Articles by Shurin, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shurin, G. V.
Right arrow Articles by Shurin, M. R.
The Journal of Immunology, 2005, 174: 5490-5498.
Copyright © 2005 by The American Association of Immunologists

Loss of New Chemokine CXCL14 in Tumor Tissue Is Associated with Low Infiltration by Dendritic Cells (DC), while Restoration of Human CXCL14 Expression in Tumor Cells Causes Attraction of DC Both In Vitro and In Vivo1

Galina V. Shurin*, Robert Ferris{dagger},§, Irina L. Tourkova*, Lori Perez*, Anna Lokshin*, Levent Balkir*, Bobby Collins, Gurkamal S. Chatta{ddagger} and Michael R. Shurin2,*,{dagger}

Departments of * Pathology, {dagger} Immunology, {ddagger} Medicine, and § Otolaryngology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213; and Department of Oral Medicine and Pathology, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA 15261

Breast and kidney-expressed chemokine (BRAK) CXCL14 is a new CXC chemokine with unknown function and receptor selectivity. The majority of head and neck squamous cell carcinoma (HNSCC) and some cervical squamous cell carcinoma do not express CXCL14 mRNA, as opposed to constitutive expression by normal oral squamous epithelium. In this study, we demonstrate that the loss of CXCL14 in HNSCC cells and at HNSCC primary tumor sites was correlated with low or no attraction of dendritic cell (DC) in vitro, and decreased infiltration of HNSCC mass by DC at the tumor site in vivo. Next, we found that recombinant human CXCL14 and CXCL14-positive HNSCC cell lines induced DC attraction in vitro, whereas CXCL14-negative HNSCC cells did not chemoattract DC. Transduction of CXCL14-negative HNSCC cell lines with the human CXCL14 gene resulted in stimulation of DC attraction in vitro and increased tumor infiltration by DC in vivo in chimeric animal models. Furthermore, evaluating the biologic effect of CXCL14 on DC, we demonstrated that the addition of recombinant human CXCL14 to DC cultures resulted in up-regulation of the expression of DC maturation markers, as well as enhanced proliferation of allogeneic T cells in MLR. Activation of DC with recombinant human CXCL14 was accompanied by up-regulation of NF-{kappa}B activity. These data suggest that CXCL14 is a potent chemoattractant and activator of DC and might be involved in DC homing in vivo.




This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
M. C. Satterfield, G. Song, K. J. Kochan, P. K. Riggs, R. M. Simmons, C. G. Elsik, D. L. Adelson, F. W. Bazer, H. Zhou, and T. E. Spencer
Discovery of candidate genes and pathways in the endometrium regulating ovine blastocyst growth and conceptus elongation
Physiol Genomics, October 1, 2009; 39(2): 85 - 99.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Salogni, T. Musso, D. Bosisio, M. Mirolo, V. R. Jala, B. Haribabu, M. Locati, and S. Sozzani
Activin A induces dendritic cell migration through the polarized release of CXC chemokine ligands 12 and 14
Blood, June 4, 2009; 113(23): 5848 - 5856.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Pelicano, W. Lu, Y. Zhou, W. Zhang, Z. Chen, Y. Hu, and P. Huang
Mitochondrial Dysfunction and Reactive Oxygen Species Imbalance Promote Breast Cancer Cell Motility through a CXCL14-Mediated Mechanism
Cancer Res., March 15, 2009; 69(6): 2375 - 2383.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Augsten, C. Hagglof, E. Olsson, C. Stolz, P. Tsagozis, T. Levchenko, M. J. Frederick, A. Borg, P. Micke, L. Egevad, et al.
CXCL14 is an autocrine growth factor for fibroblasts and acts as a multi-modal stimulator of prostate tumor growth
PNAS, March 3, 2009; 106(9): 3414 - 3419.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Maerki, S. Meuter, M. Liebi, K. Muhlemann, M. J. Frederick, N. Yawalkar, B. Moser, and M. Wolf
Potent and Broad-Spectrum Antimicrobial Activity of CXCL14 Suggests an Immediate Role in Skin Infections
J. Immunol., January 1, 2009; 182(1): 507 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Bloushtain-Qimron, J. Yao, E. L. Snyder, M. Shipitsin, L. L. Campbell, S. A. Mani, M. Hu, H. Chen, V. Ustyansky, J. E. Antosiewicz, et al.
Cell type-specific DNA methylation patterns in the human breast
PNAS, September 16, 2008; 105(37): 14076 - 14081.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
C. G. Kleer, N. Bloushtain-Qimron, Y.-H. Chen, D. Carrasco, M. Hu, J. Yao, S.-K. Kraeft, L. C. Collins, M. S. Sabel, P. Argani, et al.
Epithelial and Stromal Cathepsin K and CXCL14 Expression in Breast Tumor Progression
Clin. Cancer Res., September 1, 2008; 14(17): 5357 - 5367.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. Oler, C. P. Camacho, F. C. Hojaij, P. Michaluart Jr., G. J. Riggins, and J. M. Cerutti
Gene Expression Profiling of Papillary Thyroid Carcinoma Identifies Transcripts Correlated with BRAF Mutational Status and Lymph Node Metastasis
Clin. Cancer Res., August 1, 2008; 14(15): 4735 - 4742.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Nara, Y. Nakayama, S. Okamoto, H. Tamura, M. Kiyono, M. Muraoka, K. Tanaka, C. Taya, H. Shitara, R. Ishii, et al.
Disruption of CXC Motif Chemokine Ligand-14 in Mice Ameliorates Obesity-induced Insulin Resistance
J. Biol. Chem., October 19, 2007; 282(42): 30794 - 30803.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Lee, D. Medina, A. Tsimelzon, S. K. Mohsin, S. Mao, Y. Wu, and D. C. Allred
Alterations of Gene Expression in the Development of Early Hyperplastic Precursors of Breast Cancer
Am. J. Pathol., July 1, 2007; 171(1): 252 - 262.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Pescatori, A. Broccolini, C. Minetti, E. Bertini, C. Bruno, A. D'amico, C. Bernardini, M. Mirabella, G. Silvestri, V. Giglio, et al.
Gene expression profiling in the early phases of DMD: a constant molecular signature characterizes DMD muscle from early postnatal life throughout disease progression
FASEB J, April 1, 2007; 21(4): 1210 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Meuter, P. Schaerli, R. S. Roos, O. Brandau, M. R. Bosl, U. H. von Andrian, and B. Moser
Murine CXCL14 Is Dispensable for Dendritic Cell Function and Localization within Peripheral Tissues
Mol. Cell. Biol., February 1, 2007; 27(3): 983 - 992.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Talbi, A. E. Hamilton, K. C. Vo, S. Tulac, M. T. Overgaard, C. Dosiou, N. Le Shay, C. N. Nezhat, R. Kempson, B. A. Lessey, et al.
Molecular Phenotyping of Human Endometrium Distinguishes Menstrual Cycle Phases and Underlying Biological Processes in Normo-Ovulatory Women
Endocrinology, March 1, 2006; 147(3): 1097 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. H. Durand, V. Flacher, A. Romeas, F. Carrouel, E. Colomb, C. Vincent, H. Magloire, M.-L. Couble, F. Bleicher, M.-J. Staquet, et al.
Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts.
J. Immunol., March 1, 2006; 176(5): 2880 - 2887.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.