HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Phan, T. G. Articles by Brink, R. Search for Related Content PubMed PubMed Citation Articles by Phan, T. G. Articles by Brink, R.

Altered Migration, Recruitment, and Somatic Hypermutation in the Early Response of Marginal Zone B Cells to T Cell-Dependent Antigen¹

Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia

The early responses of follicular (Fo) and marginal zone (MZ) B cells to T cell-dependent Ag were compared using anti-hen egg lysozyme (HEL⁺) B cells capable of class switch recombination and somatic hypermutation (SHM). Purified CD21/35^intCD23^high Fo and CD21/35^highCD23^low MZ splenic B cells from SW_HEL Ig-transgenic mice were transferred into wild-type recipients and challenged with HEL-sheep RBC. Responding HEL⁺ B cells from both populations switched efficiently to IgG1, generated syndecan-1⁺ Ab-secreting cells, and exhibited equivalent rates of proliferation. However, the expansion of HEL⁺ MZ B cells lagged significantly behind that of HEL⁺ Fo B cells due to less efficient homing to the outer periarteriolar lymphatic sheath and reduced recruitment into the proliferative response. Despite the equivalent rates of class switch recombination, the onset of SHM was delayed in the MZ subset, indicating that these two activation-induced cytidine deaminase-dependent events are uncoupled in the early response of MZ B cells. Migration of HEL⁺ B cells into germinal centers coincided with the onset of SHM, occurring more rapidly with Fo vs MZ responders. These results are consistent with the concept that Fo and MZ B cells have evolved to specialize in T cell-dependent and T-independent responses respectively.

This article has been cited by other articles:

				C. A. Durand, K. Hartvigsen, L. Fogelstrand, S. Kim, S. Iritani, B. Vanhaesebroeck, J. L. Witztum, K. D. Puri, and M. R. Gold Phosphoinositide 3-Kinase p110{delta} Regulates Natural Antibody Production, Marginal Zone and B-1 B Cell Function, and Autoantibody Responses J. Immunol., November 1, 2009; 183(9): 5673 - 5684. [Abstract] [Full Text] [PDF]

				T. D. Chan, D. Gatto, K. Wood, T. Camidge, A. Basten, and R. Brink Antigen Affinity Controls Rapid T-Dependent Antibody Production by Driving the Expansion Rather than the Differentiation or Extrafollicular Migration of Early Plasmablasts J. Immunol., September 1, 2009; 183(5): 3139 - 3149. [Abstract] [Full Text] [PDF]

				G. Chattopadhyay, Q. Chen, J. Colino, A. Lees, and C. M. Snapper Intact Bacteria Inhibit the Induction of Humoral Immune Responses to Bacterial-Derived and Heterologous Soluble T Cell-Dependent Antigens J. Immunol., February 15, 2009; 182(4): 2011 - 2019. [Abstract] [Full Text] [PDF]

				Q. Chen, J. L. Cannons, J. C. Paton, H. Akiba, P. L. Schwartzberg, and C. M. Snapper A Novel ICOS-Independent, but CD28- and SAP-Dependent, Pathway of T Cell-Dependent, Polysaccharide-Specific Humoral Immunity in Response to Intact Streptococcus pneumoniae versus Pneumococcal Conjugate Vaccine J. Immunol., December 15, 2008; 181(12): 8258 - 8266. [Abstract] [Full Text] [PDF]

				A. V. Rubtsov, C. L. Swanson, S. Troy, P. Strauch, R. Pelanda, and R. M. Torres TLR Agonists Promote Marginal Zone B Cell Activation and Facilitate T-Dependent IgM Responses J. Immunol., March 15, 2008; 180(6): 3882 - 3888. [Abstract] [Full Text] [PDF]

				S. G. Tangye and K. L. Good Human IgM+CD27+ B Cells: Memory B Cells or "Memory" B Cells? J. Immunol., July 1, 2007; 179(1): 13 - 19. [Abstract] [Full Text] [PDF]

				M. Thomas, M. Calamito, B. Srivastava, I. Maillard, W. S. Pear, and D. Allman Notch activity synergizes with B-cell-receptor and CD40 signaling to enhance B-cell activation Blood, April 15, 2007; 109(8): 3342 - 3350. [Abstract] [Full Text] [PDF]

				T. G. Phan, D. Paus, T. D. Chan, M. L. Turner, S. L. Nutt, A. Basten, and R. Brink High affinity germinal center B cells are actively selected into the plasma cell compartment J. Exp. Med., October 30, 2006; 203(11): 2419 - 2424. [Abstract] [Full Text] [PDF]

				D. Paus, T. G. Phan, T. D. Chan, S. Gardam, A. Basten, and R. Brink Antigen recognition strength regulates the choice between extrafollicular plasma cell and germinal center B cell differentiation J. Exp. Med., April 17, 2006; 203(4): 1081 - 1091. [Abstract] [Full Text] [PDF]