| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Enhances TLR3-Mediated Antiviral Cytokine Expression in Human Endothelial and Epithelial Cells by Up-Regulating TLR3 Expression1
* Department of Microbiology, National Public Health Institute, Helsinki, Finland; and
Haartman Institute, Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland
TLRs play a critical role in early innate immune response to virus infection. TLR3 together with TLR7 and TLR8 constitute a powerful system to detect genetic material of RNA viruses. TLR3 has been shown to bind viral dsRNA whereas TLR7 and TLR8 are receptors for viral single-stranded RNA. In this report we show that TLR7 or TLR8 are not expressed in human epithelial A549 cells or in HUVECs. Accordingly, A549 cells and HUVECs were unresponsive to TLR7/8 ligand R848. TLR3 was expressed at a higher level in HUVECs than in A549 cells. The TLR3 ligand poly(I:C) up-regulated IFN-
, IL-28, IL-29, STAT1, and TLR3 expression in HUVECs but not in A549 cells. An enhanced TLR3 expression by transfection or by IFN-
stimulation conferred poly(I:C) responsiveness in A549 cells. Similarly, IFN- pretreatment strongly enhanced poly(I:C)-induced activation of IFN-, IL-28, and IL-29 genes also in HUVECs. In conclusion, our results suggest that IFN--induced up-regulation of TLR3 expression is involved in dsRNA activated antiviral response in human epithelial and endothelial cells.
This article has been cited by other articles:
|
|
 |
|
  H. Hagele, R. Allam, R. D. Pawar, and H.-J. Anders Double-stranded RNA activates type I interferon secretion in glomerular endothelial cells via retinoic acid-inducible gene (RIG)-1 Nephrol. Dial. Transplant., November 1, 2009; 24(11): 3312 - 3318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Welte, K. Reagan, H. Fang, C. Machain-Williams, X. Zheng, N. Mendell, G.-J. J. Chang, P. Wu, C. D. Blair, and T. Wang Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection J. Gen. Virol., November 1, 2009; 90(11): 2660 - 2668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Flur, R. Allam, D. Zecher, O. P. Kulkarni, J. Lichtnekert, M. Schwarz, B. Beutler, V. Vielhauer, and H.-J. Anders Viral RNA Induces Type I Interferon-Dependent Cytokine Release and Cell Death in Mesangial Cells via Melanoma-Differentiation-Associated Gene-5: Implications for Viral Infection-Associated Glomerulonephritis Am. J. Pathol., November 1, 2009; 175(5): 2014 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wang, Y. Liang, S. Devaraj, J. Wang, S. M. Lemon, and K. Li Toll-Like Receptor 3 Mediates Establishment of an Antiviral State against Hepatitis C Virus in Hepatoma Cells J. Virol., October 1, 2009; 83(19): 9824 - 9834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, X. Liu, Y. Zhou, and S. B. Su Interferon-{lambda}s: the modulators of antivirus, antitumor, and immune responses J. Leukoc. Biol., July 1, 2009; 86(1): 23 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Romieu-Mourez, M. Francois, M.-N. Boivin, M. Bouchentouf, D. E. Spaner, and J. Galipeau Cytokine Modulation of TLR Expression and Activation in Mesenchymal Stromal Cells Leads to a Proinflammatory Phenotype J. Immunol., June 15, 2009; 182(12): 7963 - 7973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Parker, E. C. Prestwich, J. R. Ward, E. Smythe, A. Berry, M. Triantafilou, K. Triantafilou, and I. Sabroe A Phosphatidylserine Species Inhibits a Range of TLR- but Not IL-1{beta}-Induced Inflammatory Responses by Disruption of Membrane Microdomains J. Immunol., October 15, 2008; 181(8): 5606 - 5617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. N. Kalali, G. Kollisch, J. Mages, T. Muller, S. Bauer, H. Wagner, J. Ring, R. Lang, M. Mempel, and M. Ollert Double-Stranded RNA Induces an Antiviral Defense Status in Epidermal Keratinocytes through TLR3-, PKR-, and MDA5/RIG-I-Mediated Differential Signaling J. Immunol., August 15, 2008; 181(4): 2694 - 2704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Morikawa, A. Sugiyama, H. Kume, S. Ota, T. Kashima, K. Tomita, T. Kitamura, T. Kodama, M. Fukayama, and H. Aburatani Identification of Toll-Like Receptor 3 as a Potential Therapeutic Target in Clear Cell Renal Cell Carcinoma Clin. Cancer Res., October 1, 2007; 13(19): 5703 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Salaun, S. Lebecque, S. Matikainen, D. Rimoldi, and P. Romero Toll-like Receptor 3 Expressed by Melanoma Cells as a Target for Therapy? Clin. Cancer Res., August 1, 2007; 13(15): 4565 - 4574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Danese, E. Dejana, and C. Fiocchi Immune Regulation by Microvascular Endothelial Cells: Directing Innate and Adaptive Immunity, Coagulation, and Inflammation J. Immunol., May 15, 2007; 178(10): 6017 - 6022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Mayer, M. Muehmer, J. Mages, K. Gueinzius, C. Hess, K. Heeg, R. Bals, R. Lang, and A. H. Dalpke Differential Recognition of TLR-Dependent Microbial Ligands in Human Bronchial Epithelial Cells J. Immunol., March 1, 2007; 178(5): 3134 - 3142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Liu, M. Jamaluddin, K. Li, R. P. Garofalo, A. Casola, and A. R. Brasier Retinoic Acid-Inducible Gene I Mediates Early Antiviral Response and Toll-Like Receptor 3 Expression in Respiratory Syncytial Virus-Infected Airway Epithelial Cells J. Virol., February 1, 2007; 81(3): 1401 - 1411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Paladino, D. T. Cummings, R. S. Noyce, and K. L. Mossman The IFN-Independent Response to Virus Particle Entry Provides a First Line of Antiviral Defense That Is Independent of TLRs and Retinoic Acid-Inducible Gene I J. Immunol., December 1, 2006; 177(11): 8008 - 8016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yang, V. Murthy, K. Schultz, J. B. Tatro, K. A. Fitzgerald, and D. Beasley Toll-like receptor 3 signaling evokes a proinflammatory and proliferative phenotype in human vascular smooth muscle cells Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2334 - H2343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heidemann, W. Domschke, T. Kucharzik, and C. Maaser Intestinal microvascular endothelium and innate immunity in inflammatory bowel disease: a second line of defense? Infect. Immun., October 1, 2006; 74(10): 5425 - 5432. [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Morris, L. C. Parker, J. R. Ward, E. C. Jones, M. K. B. Whyte, C. E. Brightling, P. Bradding, S. K. Dower, and I. Sabroe Cooperative molecular and cellular networks regulate Toll-like receptor-dependent inflammatory responses FASEB J, October 1, 2006; 20(12): 2153 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Huang, K. E. Duffy, L. R. San Mateo, B. Y. Amegadzie, R. T. Sarisky, and M. L. Mbow A pathway analysis of poly(I:C)-induced global gene expression change in human peripheral blood mononuclear cells Physiol Genomics, September 14, 2006; 26(2): 125 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Pawar, P. S. Patole, M. Wornle, and H.-J. Anders Microbial nucleic acids pay a Toll in kidney disease Am J Physiol Renal Physiol, September 1, 2006; 291(3): F509 - F516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ciencewicki, L. Brighton, W.-D. Wu, M. Madden, and I. Jaspers Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1154 - L1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. Harari, P. Alcaide, D. Ahl, F. W. Luscinskas, and J. K. Liao Absence of TRAM Restricts Toll-Like Receptor 4 Signaling in Vascular Endothelial Cells to the MyD88 Pathway Circ. Res., May 12, 2006; 98(9): 1134 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matikainen, J. Siren, J. Tissari, V. Veckman, J. Pirhonen, M. Severa, Q. Sun, R. Lin, S. Meri, G. Uze, et al. Tumor Necrosis Factor Alpha Enhances Influenza A Virus-Induced Expression of Antiviral Cytokines by Activating RIG-I Gene Expression. J. Virol., April 1, 2006; 80(7): 3515 - 3522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Groskreutz, M. M. Monick, L. S. Powers, T. O. Yarovinsky, D. C. Look, and G. W. Hunninghake Respiratory Syncytial Virus Induces TLR3 Protein and Protein Kinase R, Leading to Increased Double-Stranded RNA Responsiveness in Airway Epithelial Cells J. Immunol., February 1, 2006; 176(3): 1733 - 1740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Sanghavi and T. A. Reinhart Increased Expression of TLR3 in Lymph Nodes during Simian Immunodeficiency Virus Infection: Implications for Inflammation and Immunodeficiency J. Immunol., October 15, 2005; 175(8): 5314 - 5323. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
