HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Related articles in The JI Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allen, L.-A. H. Articles by Wittine, L. M. Search for Related Content PubMed PubMed Citation Articles by Allen, L.-A. H. Articles by Wittine, L. M.

Helicobacter pylori Disrupts NADPH Oxidase Targeting in Human Neutrophils to Induce Extracellular Superoxide Release¹

Department of Medicine and Inflammation Program, University of Iowa, Coralville, IA 52241; and the Veterans Affairs Medical Center, Iowa City, IA 52246

Helicobacter pylori (Hp) infection triggers a chronic influx of polymorphonuclear leukocyte neutrophils (PMNs) into the gastric mucosa. Although Hp reside in a neutrophil-rich environment, how these organisms evade phagocytic killing is largely unexplored. We now show that live Hp (strains 11637, 60190, DT61A, and 11916) are readily ingested by PMNs and induce a rapid and strong respiratory burst that is comparable to PMA. Relative to other particulate stimuli, Hp are more potent activators of PMNs than opsonized zymosan, Staphylococcus aureus, or Salmonella. Strikingly, biochemical and microscopic analyses demonstrate that Hp disrupt NADPH oxidase targeting such that superoxide anions are released into the extracellular milieu and do not accumulate inside Hp phagosomes. Specifically, nascent Hp phagosomes acquire flavocytochrome b₅₅₈ but do not efficiently recruit or retain p47^phox or p67^phox. Superoxide release peaks at 16 min coincident with the appearance of assembled oxidase complexes in patches at the cell surface. Oxidant release is regulated by formalin-resistant and heat-sensitive bacterial surface factors distinct from urease and Hp(2–20). Following opsonization with fresh serum, Hp triggers a modest respiratory burst that is confined to the phagosome, and ingested bacteria are eliminated. We conclude that disruption of NADPH oxidase targeting allows unopsonized Hp to escape phagocytic killing, and our findings support the hypothesis that bacteria and PMNs act in concert to damage the gastric mucosa.

Related articles in The JI:

IN THIS ISSUE

The JI 2005 174: 3135-3136. [Full Text]  

In This Issue

The JI 2005 174: 5135. [Full Text]  

This article has been cited by other articles:

				S. K. Amundsen, J. Fero, N. R. Salama, and G. R. Smith Dual Nuclease and Helicase Activities of Helicobacter pylori AddAB Are Required for DNA Repair, Recombination, and Mouse Infectivity J. Biol. Chem., June 19, 2009; 284(25): 16759 - 16766. [Abstract] [Full Text] [PDF]

				B. W. Buchan, R. L. McCaffrey, S. R. Lindemann, L.-A. H. Allen, and B. D. Jones Identification of migR, a Regulatory Element of the Francisella tularensis Live Vaccine Strain iglABCD Virulence Operon Required for Normal Replication and Trafficking in Macrophages Infect. Immun., June 1, 2009; 77(6): 2517 - 2529. [Abstract] [Full Text] [PDF]

				K. E. Keith, D. W. Hynes, J. E. Sholdice, and M. A. Valvano Delayed association of the NADPH oxidase complex with macrophage vacuoles containing the opportunistic pathogen Burkholderia cenocepacia Microbiology, April 1, 2009; 155(4): 1004 - 1015. [Abstract] [Full Text] [PDF]

				G. S. Schulert, R. L. McCaffrey, B. W. Buchan, S. R. Lindemann, C. Hollenback, B. D. Jones, and L.-A. H. Allen Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS Infect. Immun., April 1, 2009; 77(4): 1324 - 1336. [Abstract] [Full Text] [PDF]

				A.-J. Casbon, L.-A. H. Allen, K. W. Dunn, and M. C. Dinauer Macrophage NADPH Oxidase Flavocytochrome b Localizes to the Plasma Membrane and Rab11-Positive Recycling Endosomes J. Immunol., February 15, 2009; 182(4): 2325 - 2339. [Abstract] [Full Text] [PDF]

				M. Wellington, K. Dolan, and D. J. Krysan Live Candida albicans Suppresses Production of Reactive Oxygen Species in Phagocytes Infect. Immun., January 1, 2009; 77(1): 405 - 413. [Abstract] [Full Text] [PDF]

				J. L. Spinner, J. A. Cundiff, and S. D. Kobayashi Yersinia pestis Type III Secretion System-Dependent Inhibition of Human Polymorphonuclear Leukocyte Function Infect. Immun., August 1, 2008; 76(8): 3754 - 3760. [Abstract] [Full Text] [PDF]

				R. L. McCaffrey and L.-A. H. Allen Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape J. Leukoc. Biol., December 1, 2006; 80(6): 1224 - 1230. [Abstract] [Full Text] [PDF]

				H. M. S. Algood and T. L. Cover Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses Clin. Microbiol. Rev., October 1, 2006; 19(4): 597 - 613. [Abstract] [Full Text] [PDF]

				G. S. Schulert and L.-A. H. Allen Differential infection of mononuclear phagocytes by Francisella tularensis: role of the macrophage mannose receptor J. Leukoc. Biol., September 1, 2006; 80(3): 563 - 571. [Abstract] [Full Text] [PDF]

				J. T. Schwartz and L.-A. H. Allen Role of urease in megasome formation and Helicobacter pylori survival in macrophages J. Leukoc. Biol., June 1, 2006; 79(6): 1214 - 1225. [Abstract] [Full Text] [PDF]