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3' Enhancer Is Activated by Gradients of Chromatin Accessibility and Protein Association1
* Department of Medicine, Immunobiology Unit, Evans Memorial Department of Clinical Research, Boston Medical Center, Boston, MA 02118;
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104; and
Department of Microbiology, Boston University School of Medicine, Boston, MA 02118
The Ig
locus is recombined following initiation of a signaling cascade during the early pre-B stage of B cell development. The Ig 3' enhancer plays an important role in normal B cell development by regulating locus activation. Quantitative analyses of 3' enhancer chromatin structure by restriction endonuclease accessibility and protein association by chromatin immunoprecipitation in a developmental series of primary murine B cells and murine B cell lines demonstrate that the enhancer is activated progressively through multiple steps as cells mature. Moderate 3' chromatin accessibility and low levels of protein association in pro-B cells are increased substantially as the cells progress from pro- to pre-B, then eventually mature B cell stages. Chromatin immunoprecipitation assays suggest transcriptional regulators of the 3' enhancer, specifically PU.1 and IFN regulatory factor-4, exploit enhanced accessibility by increasing association as cells mature. Characterization of histone acetylation patterns at the 3' enhancer and experimental inhibition of histone deacetylation suggest changes therein may determine changes in enzyme and transcription factor accessibility. This analysis demonstrates activation is a multistep process initiated in early B cell precursors before Igµ recombination and finalized only after the pre-B cell stage.
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