HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jackson, S. M. Articles by Capra, J. D. Search for Related Content PubMed PubMed Citation Articles by Jackson, S. M. Articles by Capra, J. D.

IgH V-Region Sequence Does Not Predict the Survival Fate of Human Germinal Center B Cells¹

Molecular Immunogenetics, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104

Germinal center (GC) B cell survival fate is governed in part by the outcome of successful/failed BCR-mediated interactions with accessory cells. However, the extent to which the BCR primary sequence influences such interactions is not fully understood. Over 1000 IgV_H4 family cDNAs were sequenced from living (annexin V^–) and apoptotic (annexin V⁺ or from within tingible body macrophages) GC B cell fractions from seven tonsils. Results surprisingly demonstrate that living and dying GC B cells do not significantly differ in IgV_H, D, or J_H gene segment use; HCDR3 length or positive charge; or mutation frequency. Additionally, equivalent IgH cDNA sequences were identified in both fractions, suggesting that BCR sequence alone is an unreliable predictor of GC B cell survival.

This article has been cited by other articles:

				S. M. Jackson, N. Harp, D. Patel, J. Wulf, E. D. Spaeth, U. K. Dike, J. A. James, and J. D. Capra Key developmental transitions in human germinal center B cells are revealed by differential CD45RB expression Blood, April 23, 2009; 113(17): 3999 - 4007. [Abstract] [Full Text] [PDF]

				S. M. Jackson, N. Harp, D. Patel, J. Zhang, S. Willson, Y. J. Kim, C. Clanton, and J. D. Capra CD45RO enriches for activated, highly mutated human germinal center B cells Blood, December 1, 2007; 110(12): 3917 - 3925. [Abstract] [Full Text] [PDF]

				M. Shankar, J. C. Nixon, S. Maier, J. Workman, A. D. Farris, and C. F. Webb Anti-Nuclear Antibody Production and Autoimmunity in Transgenic Mice That Overexpress the Transcription Factor Bright J. Immunol., March 1, 2007; 178(5): 2996 - 3006. [Abstract] [Full Text] [PDF]

				M. Zemlin, G. Hoersch, C. Zemlin, A. Pohl-Schickinger, M. Hummel, C. Berek, R. F. Maier, and K. Bauer The Postnatal Maturation of the Immunoglobulin Heavy Chain IgG Repertoire in Human Preterm Neonates Is Slower than in Term Neonates J. Immunol., January 15, 2007; 178(2): 1180 - 1188. [Abstract] [Full Text] [PDF]

				F. Yang, G. C. Waldbieser, and C. J. Lobb The Nucleotide Targets of Somatic Mutation and the Role of Selection in Immunoglobulin Heavy Chains of a Teleost Fish J. Immunol., February 1, 2006; 176(3): 1655 - 1667. [Abstract] [Full Text] [PDF]