The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Related articles in The JI
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cong, Y.
Right arrow Articles by Elson, C. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cong, Y.
Right arrow Articles by Elson, C. O.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
The Journal of Immunology, 2005, 174: 2787-2795.
Copyright © 2005 by The American Association of Immunologists

Generation of Antigen-Specific, Foxp3-Expressing CD4+ Regulatory T Cells by Inhibition of APC Proteosome Function1

Yingzi Cong*, Astrid Konrad*, Nuzhat Iqbal*, Robin D. Hatton{dagger}, Casey T. Weaver{dagger} and Charles O. Elson2,*

* Division of Gastroenterology and Hepatology, Department of Medicine and {dagger} Department of Pathology, University of Alabama, Birmingham, AL 35294

We tested the hypothesis that immature APC, whose NF-{kappa}B-signaling pathway and thus maturation was blocked by the proteosome inhibitor benzyloxycarbonyl-isoleucyl-glutamyl(O-tert-butyl)-alanyl-leucinal (PSI), could be a source of Ag-specific regulatory T (Treg) cells. DO11.10 CD4+ T cells that were incubated with Ag- and PSI-pulsed APC proliferated poorly, produced less IL-2, IFN-{gamma}, and IL-10 in secondary cultures, and inhibited the response of both naive and memory CD4+ T cells stimulated by Ag-pulsed APC. The generation of PSI-APC Treg cells required IL-10 production by APC. PSI-APC Treg cell inhibition required cell-cell contact but not IL-10 or TGF-{beta}. Addition of IL-2 did not reverse, but Ab to CTLA-4 did reverse partially the inhibitory effect. Depletion of CD25+ T cells before initial culture with PSI-APC did not affect Treg generation. PSI-APC Treg cells expressed high levels of Foxp3, inhibited proliferation of naive DO11.10 T cells in vivo, and abrogated colitis driven by a memory Th1 response to bacterial-associated Ag. We conclude that NF-{kappa}B-blocked, immature APC are able to induce the differentiation of Treg cells that can function in vitro and in vivo in an Ag-specific manner.


Related articles in The JI:

IN THIS ISSUE

The JI 2005 174: 2447-2449. [Full Text]  



This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
P. Meier, D. Golshayan, E. Blanc, M. Pascual, and M. Burnier
Oxidized LDL Modulates Apoptosis of Regulatory T Cells in Patients with ESRD
J. Am. Soc. Nephrol., June 1, 2009; 20(6): 1368 - 1384.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. F. George, A. Braun, T. M. Brusko, R. Joseph, S. Bolisetty, C. H. Wasserfall, M. A. Atkinson, A. Agarwal, and M. H. Kapturczak
Suppression by CD4+CD25+ Regulatory T Cells Is Dependent on Expression of Heme Oxygenase-1 in Antigen-Presenting Cells
Am. J. Pathol., July 1, 2008; 173(1): 154 - 160.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Qin, K. L. Roberts, S. A. Niyongere, Y. Cong, C. O. Elson, and E. N. Benveniste
Molecular Mechanism of Lipopolysaccharide-Induced SOCS-3 Gene Expression in Macrophages and Microglia
J. Immunol., November 1, 2007; 179(9): 5966 - 5976.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Deng, D. J. Moore, X. Huang, M.-M. Lian, M. Mohiuddin, E. Velededeoglu, M. K. Lee IV, S. Sonawane, J. Kim, J. Wang, et al.
Cutting Edge: Transplant Tolerance Induced by Anti-CD45RB Requires B Lymphocytes
J. Immunol., May 15, 2007; 178(10): 6028 - 6032.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Bochtler, C. Wahl, R. Schirmbeck, and J. Reimann
Functional Adaptive CD4 Foxp3 T Cells Develop in MHC Class II-Deficient Mice
J. Immunol., December 15, 2006; 177(12): 8307 - 8314.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Fallarino, U. Grohmann, S. You, B. C. McGrath, D. R. Cavener, C. Vacca, C. Orabona, R. Bianchi, M. L. Belladonna, C. Volpi, et al.
The Combined Effects of Tryptophan Starvation and Tryptophan Catabolites Down-Regulate T Cell Receptor {zeta}-Chain and Induce a Regulatory Phenotype in Naive T Cells.
J. Immunol., June 1, 2006; 176(11): 6752 - 6761.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. Benigni, S. Tomasoni, L. A. Turka, L. Longaretti, L. Zentilin, M. Mister, A. Pezzotta, N. Azzollini, M. Noris, S. Conti, et al.
Adeno-Associated Virus-Mediated CTLA4Ig Gene Transfer Protects MHC-Mismatched Renal Allografts from Chronic Rejection
J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1665 - 1672.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
R Duchmann and M Zeitz
T regulatory cell suppression of colitis: the role of TGF-{beta}
Gut, May 1, 2006; 55(5): 604 - 606.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
S. G. Zheng, J. H. Wang, W. Stohl, K. S. Kim, J. D. Gray, and D. A. Horwitz
TGF-beta Requires CTLA-4 Early after T Cell Activation to Induce FoxP3 and Generate Adaptive CD4+CD25+ Regulatory Cells
J. Immunol., March 15, 2006; 176(6): 3321 - 3329.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.