HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hsu, K. Articles by Geczy, C. L. Search for Related Content PubMed PubMed Citation Articles by Hsu, K. Articles by Geczy, C. L.

Regulation of S100A8 by Glucocorticoids¹

Kenneth Hsu^*, Robert J. Passey^2,*, Yasumi Endoh^*, Farid Rahimi^*, Peter Youssef, Tina Yen^3,* and Carolyn L. Geczy^4,*

^* Inflammatory Diseases Research Unit, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia; and Department of Rheumatology, Institute of Rheumatology and Orthopaedics, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia

S100A8 (A8) has roles in inflammation, differentiation and development and is associated with oxidative defense. Murine A8 (mA8) is up-regulated in macrophages, fibroblasts, and microvascular endothelial cells by LPS. Glucocorticoids (GCs) amplified LPS-induced mA8 in these cells. Relative to stimulation by LPS, GCs increased mA8 gene transcription and mRNA half-life. Enhancement required new protein synthesis, IL-10 and products of the cyclooxygenase-2 pathway, and both ERK1/2 and p38 MAPK. Protein kinase A positively and protein kinase C negatively regulated this process. Promoter analysis indicated element(s) essential for LPS and dexamethasone enhancement colocated within the region –178 to 0 bp. In the absence of glucocorticoid response elements, NF1 motif at –58 is a candidate for mediation of enhancement. Gel shift analysis detected no differences between LPS- and LPS/dexamethasone-treated complexes within this region. GCs increased constitutive levels of A8 and S100A9 (A9) mRNA in human monocytes. The synovial membrane of rheumatoid patients treated with high dose i.v. methylprednisolone contained higher numbers of A8/A9-positive macrophages than pre- or posttreatment samples. Results support the proposal that A8 has anti-inflammatory properties that may be independent of hetero-complex formation with A9 and may also enable localized defense in the absence of overriding deleterious host responses.

This article has been cited by other articles:

				S. Y. Lim, M. J. Raftery, J. Goyette, K. Hsu, and C. L. Geczy Oxidative modifications of S100 proteins: functional regulation by redox J. Leukoc. Biol., September 1, 2009; 86(3): 577 - 587. [Abstract] [Full Text] [PDF]

				J. M. Ehrchen, C. Sunderkotter, D. Foell, T. Vogl, and J. Roth The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer J. Leukoc. Biol., September 1, 2009; 86(3): 557 - 566. [Abstract] [Full Text] [PDF]

				L. Chung, A. E. Nelson, K. K. Y. Ho, and R. C. Baxter Proteomic Profiling of Growth Hormone-Responsive Proteins in Human Peripheral Blood Leukocytes J. Clin. Endocrinol. Metab., August 1, 2009; 94(8): 3038 - 3043. [Abstract] [Full Text] [PDF]

				J. Goyette, W. X. Yan, E. Yamen, Y. M. Chung, S. Y. Lim, K. Hsu, F. Rahimi, N. Di Girolamo, C. Song, W. Jessup, et al. Pleiotropic Roles of S100A12 in Coronary Atherosclerotic Plaque Formation and Rupture J. Immunol., July 1, 2009; 183(1): 593 - 603. [Abstract] [Full Text] [PDF]

				Y. Endoh, Y. M. Chung, I. A. Clark, C. L. Geczy, and K. Hsu IL-10-Dependent S100A8 Gene Induction in Monocytes/Macrophages by Double-Stranded RNA J. Immunol., February 15, 2009; 182(4): 2258 - 2268. [Abstract] [Full Text] [PDF]

				S. Y. Lim, M. Raftery, H. Cai, K. Hsu, W. X. Yan, H.-L. Hseih, R. N. Watts, D. Richardson, S. Thomas, M. Perry, et al. S-Nitrosylated S100A8: Novel Anti-Inflammatory Properties J. Immunol., October 15, 2008; 181(8): 5627 - 5636. [Abstract] [Full Text] [PDF]

				R. T. Sasmono, A. Ehrnsperger, S. L. Cronau, T. Ravasi, R. Kandane, M. J. Hickey, A. D. Cook, S. R. Himes, J. A. Hamilton, and D. A. Hume Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1 J. Leukoc. Biol., July 1, 2007; 82(1): 111 - 123. [Abstract] [Full Text] [PDF]

				D. Viemann, K. Barczyk, T. Vogl, U. Fischer, C. Sunderkotter, K. Schulze-Osthoff, and J. Roth MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and -independent cell death program Blood, March 15, 2007; 109(6): 2453 - 2460. [Abstract] [Full Text] [PDF]

				H. Y. Sroussi, J. Berline, and J. M. Palefsky Oxidation of methionine 63 and 83 regulates the effect of S100A9 on the migration of neutrophils in vitro J. Leukoc. Biol., March 1, 2007; 81(3): 818 - 824. [Abstract] [Full Text] [PDF]