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The Journal of Immunology, 2005, 174: 7904-7911.
Copyright © 2005 by The American Association of Immunologists

Exacerbated Susceptibility to Infection-Stimulated Immunopathology in CD1d-Deficient Mice1

Stephen T. Smiley*, Paula A. Lanthier*, Kevin N. Couper*, Frank M. Szaba*, Jonathan E. Boyson{dagger}, Wangxue Chen{ddagger} and Lawrence L. Johnson2,*

* Trudeau Institute, Inc., Saranac Lake, NY 12983; {dagger} Department of Surgery, Division of Transplantation Surgery and Immunology, University of Vermont, Burlington, VT 05405; and {ddagger} Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada

Mice lacking functional CD1d genes were used to study mechanisms of resistance to the protozoan parasite Toxoplasma gondii. Wild-type (WT) BALB/c mice, CD1d-deficient BALB/c mice, and WT C57BL/6 mice all survived an acute oral infection with a low dose of mildly virulent strain ME49 T. gondii cysts. In contrast, most CD1d-deficient C57BL/6 mice died within 2 wk of infection. Despite having parasite burdens that were only slightly higher than WT mice, CD1d-deficient C57BL/6 mice displayed greater weight loss and intestinal pathology. In C57BL/6 mice, CD4+ cells can cause intestinal pathology during T. gondii infection. Compared with WT mice, infected CD1d-deficient C57BL/6 mice had higher frequencies and numbers of activated (CD44high) CD4+ cells in mesenteric lymph nodes. Depletion of CD4+ cells from CD1d-deficient mice reduced weight loss and prolonged survival, demonstrating a functional role for CD4+ cells in their increased susceptibility to T. gondii infection. CD1d-deficient mice are deficient in V{alpha}14+ T cells, a major population of NKT cells. Involvement of these cells in resistance to T. gondii was investigated using gene-targeted J{alpha}18-deficient C57BL/6 mice, which are deficient in V{alpha}14+ T cells. These mice did not succumb to acute infection, but experienced greater weight loss and more deaths than B6 mice during chronic infection, indicating that V{alpha}14+ cells contribute to resistance to T. gondii. The data identify CD4+ cells as a significant component of the marked susceptibility to T. gondii infection observed in CD1d-deficient C57BL/6mice, and establish T. gondii as a valuable tool for deciphering CD1d-dependent protective mechanisms.




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