The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ivanov, I. I.
Right arrow Articles by Schroeder, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ivanov, I. I.
Right arrow Articles by Schroeder, H. W., Jr.
The Journal of Immunology, 2005, 174: 7773-7780.
Copyright © 2005 by The American Association of Immunologists

Development of the Expressed Ig CDR-H3 Repertoire Is Marked by Focusing of Constraints in Length, Amino Acid Use, and Charge That Are First Established in Early B Cell Progenitors1

Ivaylo I. Ivanov2,*, Robert L. Schelonka2,{dagger}, Yingxin Zhuang{ddagger}, G. Larry Gartland*, Michael Zemlin§ and Harry W. Schroeder, Jr.3,*,{ddagger}

Departments of* Microbiology, {dagger} Pediatrics, and {ddagger} Medicine, University of Alabama at Birmingham, Birmingham, AL 35294; and § Department of Pediatrics, Philipps Universität Marburg, Marburg, Germany

To gain insight into the mechanisms that regulate the development of the H chain CDR3 (CDR-H3), we used the scheme of Hardy to sort mouse bone marrow B lineage cells into progenitor, immature, and mature B cell fractions, and then performed sequence analysis on VH7183-containing Cµ transcripts. The essential architecture of the CDR-H3 repertoire observed in the mature B cell fraction F was already established in the early pre-B cell fraction C. These architectural features include VH gene segment use preference, DH family usage, JH rank order, predicted structures of the CDR-H3 base and loop, and the amino acid composition and average hydrophobicity of the CDR-H3 loop. With development, the repertoire was focused by eliminating outliers to what appears to be a preferred repertoire in terms of length, amino acid composition, and average hydrophobicity. Unlike humans, the average length of CDR-H3 increased during development. The majority of this increase came from enhanced preservation of JH sequence. This was associated with an increase in the prevalence of tyrosine. With an accompanying increase in glycine, a shift in hydrophobicity was observed in the CDR-H3 loop from near neutral in fraction C (–0.08 ± 0.03) to mild hydrophilic in fraction F (–0.17 ± 0.02). Fundamental constraints on the sequence and structure of CDR-H3 are thus established before surface IgM expression.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. M. Souto-Carneiro, R. Fritsch, N. Sepulveda, M. J. Lagareiro, N. Morgado, N. S. Longo, and P. E. Lipsky
The NF-{kappa}B Canonical Pathway Is Involved in the Control of the Exonucleolytic Processing of Coding Ends during V(D)J Recombination
J. Immunol., January 15, 2008; 180(2): 1040 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Lutz, W. Muller, and H.-M. Jack
VH Replacement Rescues Progenitor B Cells with Two Nonproductive VDJ Alleles
J. Immunol., November 15, 2006; 177(10): 7007 - 7014.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X.-j. Yan, E. Albesiano, N. Zanesi, S. Yancopoulos, A. Sawyer, E. Romano, A. Petlickovski, D. G. Efremov, C. M. Croce, and N. Chiorazzi
B cell receptors in TCL1 transgenic mice resemble those of aggressive, treatment-resistant human chronic lymphocytic leukemia
PNAS, August 1, 2006; 103(31): 11713 - 11718.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Watson, C. S. Moffatt-Blue, R. Z. McDonald, E. Kompfner, D. Ait-Azzouzene, D. Nemazee, A. N. Theofilopoulos, D. H. Kono, and A. J. Feeney
Paucity of V-D-D-J Rearrangements and VH Replacement Events in Lupus Prone and Nonautoimmune TdT-/- and TdT+/+ Mice
J. Immunol., July 15, 2006; 177(2): 1120 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
G. C. Ippolito, R. L. Schelonka, M. Zemlin, I. I. Ivanov, R. Kobayashi, C. Zemlin, G. L. Gartland, L. Nitschke, J. Pelkonen, K. Fujihashi, et al.
Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production
J. Exp. Med., June 12, 2006; 203(6): 1567 - 1578.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. L Cassady-Cain and A. K Kaushik
Increased negative selection impairs neonatal B cell repertoire but does not directly lead to generation of disease-associated IgM auto-antibodies
Int. Immunol., May 1, 2006; 18(5): 661 - 669.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. M. Souto-Carneiro, G. P. Sims, H. Girschik, J. Lee, and P. E. Lipsky
Developmental Changes in the Human Heavy Chain CDR3
J. Immunol., December 1, 2005; 175(11): 7425 - 7436.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. L. Schelonka, I. I. Ivanov, D. H. Jung, G. C. Ippolito, L. Nitschke, Y. Zhuang, G. L. Gartland, J. Pelkonen, F. W. Alt, K. Rajewsky, et al.
A Single DH Gene Segment Creates Its Own Unique CDR-H3 Repertoire and Is Sufficient for B cell Development and Immune Function
J. Immunol., November 15, 2005; 175(10): 6624 - 6632.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.