HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mayer, K. D. Articles by Mohrs, M. Search for Related Content PubMed PubMed Citation Articles by Mayer, K. D. Articles by Mohrs, M.

The Functional Heterogeneity of Type 1 Effector T Cells in Response to Infection Is Related to the Potential for IFN- Production¹

Katrin D. Mayer^*, Katja Mohrs^*, Sherry R. Crowe^*, Lawrence L. Johnson^*, Paul Rhyne, David L. Woodland^* and Markus Mohrs^2,*

^* Trudeau Institute, Saranac Lake, NY 12983; and Upstate Biotechnology, Lake Placid, NY 12946

The expression of IFN- is a hallmark of Th1 cells and CD8⁺ effector T cells and is the signature cytokine of type 1 responses. However, it is not known whether T cells are homogeneous in their capacity to produce IFN-, whether this potential varies between tissues, and how it relates to the production of other effector molecules. In the present study we used bicistronic IFN--enhanced yellow fluorescent protein (IFN--eYFP) reporter mice (Yeti) and MHC class I tetramers to directly quantify IFN- expression at the single cell level. The eYFP fluorescence of Th1 cells and CD8⁺ effector T cells was broadly heterogeneous even before cell division and correlated with both the abundance of IFN- transcripts and the secretion of IFN- upon stimulation. CD4⁺ and CD8⁺ T cells of influenza-infected mice revealed a similarly heterogeneous IFN- expression, and eYFP^high cells were only found in the infected lung. Ag-specific T cells were in all examined tissues eYFP⁺, but also heterogeneous in their reporter fluorescence, and eYFP^high cells were also restricted to the infected lung. A similar heterogeneity was observed in Toxoplasma gondii-infected animals, but eYFP^high cells were restricted to different tissues. Highly eYFP fluorescent cells produced elevated levels of proinflammatory cytokines and chemokines in addition to IFN-, suggesting their coregulated expression as a functional unit in highly differentiated effector T cells.

This article has been cited by other articles:

				K. N. Couper, P. A. Lanthier, G. Perona-Wright, L. W. Kummer, W. Chen, S. T. Smiley, M. Mohrs, and L. L. Johnson Anti-CD25 Antibody-Mediated Depletion of Effector T Cell Populations Enhances Susceptibility of Mice to Acute but Not Chronic Toxoplasma gondii Infection J. Immunol., April 1, 2009; 182(7): 3985 - 3994. [Abstract] [Full Text] [PDF]

				A. L. Croxford, F. C. Kurschus, and A. Waisman Cutting Edge: An IL-17F-CreEYFP Reporter Mouse Allows Fate Mapping of Th17 Cells J. Immunol., February 1, 2009; 182(3): 1237 - 1241. [Abstract] [Full Text] [PDF]

				J. J. Reece, M. C. Siracusa, T. L. Southard, C. F. Brayton, J. F. Urban Jr., and A. L. Scott Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung Infect. Immun., August 1, 2008; 76(8): 3511 - 3524. [Abstract] [Full Text] [PDF]

				C. N. Janicki, S. R. Jenkinson, N. A. Williams, and D. J. Morgan Loss of CTL Function among High-Avidity Tumor-Specific CD8+ T Cells following Tumor Infiltration Cancer Res., April 15, 2008; 68(8): 2993 - 3000. [Abstract] [Full Text] [PDF]

				K. Kalies, P. Konig, Y.-M. Zhang, M. Deierling, J. Barthelmann, C. Stamm, and J. Westermann Nonoverlapping Expression of IL10, IL12p40, and IFN{gamma} mRNA in the Marginal Zone and T Cell Zone of the Spleen after Antigenic Stimulation J. Immunol., April 15, 2008; 180(8): 5457 - 5465. [Abstract] [Full Text] [PDF]

				K. D. Mayer, K. Mohrs, W. Reiley, S. Wittmer, J. E. Kohlmeier, J. E. Pearl, A. M. Cooper, L. L. Johnson, D. L. Woodland, and M. Mohrs Cutting Edge: T-bet and IL-27R Are Critical for In Vivo IFN-{gamma} Production by CD8 T Cells during Infection J. Immunol., January 15, 2008; 180(2): 693 - 697. [Abstract] [Full Text] [PDF]

				S. Thibault, M. R. Tardif, C. Barat, and M. J. Tremblay TLR2 Signaling Renders Quiescent Naive and Memory CD4+ T Cells More Susceptible to Productive Infection with X4 and R5 HIV-Type 1 J. Immunol., October 1, 2007; 179(7): 4357 - 4366. [Abstract] [Full Text] [PDF]

				D. M. Jelley-Gibbs, J. P. Dibble, D. M. Brown, T. M. Strutt, K. K. McKinstry, and S. L. Swain Persistent Depots of Influenza Antigen Fail To Induce a Cytotoxic CD8 T Cell Response J. Immunol., June 15, 2007; 178(12): 7563 - 7570. [Abstract] [Full Text] [PDF]

				Z. Xiao, J. M. Curtsinger, M. Prlic, S. C. Jameson, and M. F. Mescher The CD8 T cell response to vaccinia virus exhibits site-dependent heterogeneity of functional responses Int. Immunol., June 1, 2007; 19(6): 733 - 743. [Abstract] [Full Text] [PDF]

				M. A. Kapina, G. S. Shepelkova, V. V. Mischenko, P. Sayles, P. Bogacheva, G. Winslow, A. S. Apt, and I. V. Lyadova CD27low CD4 T Lymphocytes That Accumulate in the Mouse Lungs during Mycobacterial Infection Differentiate from CD27high Precursors In Situ, Produce IFN-{gamma}, and Protect the Host against Tuberculosis Infection J. Immunol., January 15, 2007; 178(2): 976 - 985. [Abstract] [Full Text] [PDF]

				E. Reyes, A. Prieto, A. de la Hera, P. de Lucas, R. Alvarez-Sala, J. L. Alvarez-Sala, and M. Alvarez-Mon Treatment With AM3 Restores Defective T-Cell Function in COPD Patients Chest, March 1, 2006; 129(3): 527 - 535. [Abstract] [Full Text] [PDF]