| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
Departments of*
Biochemistry and Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, and Departments of Obstetrics and Gynecology and Internal Medicine, Tufts-New England Medical Center, Boston, MA 02111
Mast cells are critical for allergic reactions, but also for innate or acquired immunity and inflammatory conditions that worsen by stress. Corticotropin-releasing hormone (CRH), which activates the hypothalamic-pituitary-adrenal axis under stress, also has proinflammatory peripheral effects possibly through mast cells. We investigated the expression of CRH receptors and the effects of CRH in the human leukemic mast cell (HMC-1) line and human umbilical cord blood-derived mast cells. We detected mRNA for CRH-R1
, 1
, 1c, 1e, 1f isoforms, as well as CRH-R1 protein in both cell types. CRH-R2 (but not R2 or R2
) mRNA and protein were present only in human cord blood-derived mast cells. CRH increased cAMP and induced secretion of vascular endothelial growth factor (VEGF) without tryptase, histamine, IL-6, IL-8, or TNF- release. The effects were blocked by the CRH-R1 antagonist antalarmin, but not the CRH-R2 antagonist astressin 2B. CRH-stimulated VEGF production was mediated through activation of adenylate cyclase and increased cAMP, as evidenced by the fact that the effect of CRH was mimicked by the direct adenylate cyclase activator forskolin and the cell-permeable cAMP analog 8-bromo-cAMP, whereas it was abolished by the adenylate cyclase inhibitor SQ22536. This is the first evidence that mast cells express functional CRH receptors and that CRH can induce VEGF secretion selectively. CRH-induced mast cell-derived VEGF could, therefore, be involved in chronic inflammatory conditions associated with increased VEGF, such as arthritis or psoriasis, both of which worsen by stress.
This article has been cited by other articles:
|
|
 |
|
  M. Tagen, A. Elorza, D. Kempuraj, W. Boucher, C. L. Kepley, O. S. Shirihai, and T. C. Theoharides Mitochondrial Uncoupling Protein 2 Inhibits Mast Cell Activation and Reduces Histamine Content J. Immunol., November 15, 2009; 183(10): 6313 - 6319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. LOFFREDO, F. AYALA, G. MARONE, G. DELFINO, S. STRANGES, and G. MARONE Immunopathogenesis of Psoriasis and Pharmacological Perspectives J Rheumatol Suppl, August 1, 2009; 83(0): 9 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Huang, D. Kempuraj, N. Papadopoulou, T. Kourelis, J. Donelan, A. Manola, and T. C Theoharides Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-{kappa}B activation J. Mol. Endocrinol., May 1, 2009; 42(5): 397 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Teitelbaum, M. G. Gareau, J. Jury, P. C. Yang, and M. H. Perdue Chronic peripheral administration of corticotropin-releasing factor causes colonic barrier dysfunction similar to psychological stress Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G452 - G459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C H Knowles and Q Aziz Visceral hypersensitivity in non-erosive reflux disease Gut, May 1, 2008; 57(5): 674 - 683. [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Theoharides Mast Cells and Pancreatic Cancer N. Engl. J. Med., April 24, 2008; 358(17): 1860 - 1861. [Full Text] [PDF]
|
|
|
|
|
|
C Wallon, P-C Yang, A V Keita, A-C Ericson, D M McKay, P M Sherman, M H Perdue, and J D Soderholm Corticotropin-releasing hormone (CRH) regulates macromolecular permeability via mast cells in normal human colonic biopsies in vitro Gut, January 1, 2008; 57(1): 50 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Christy and M. A. Brown The Multitasking Mast Cell: Positive and Negative Roles in the Progression of Autoimmunity J. Immunol., September 1, 2007; 179(5): 2673 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D Soderholm Stress-related changes in oesophageal permeability: filling the gaps of GORD? Gut, September 1, 2007; 56(9): 1177 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Moeser, K. A. Ryan, P. K. Nighot, and A. T. Blikslager Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs Am J Physiol Gastrointest Liver Physiol, August 1, 2007; 293(2): G413 - G421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Gareau, J. Jury, and M. H. Perdue Neonatal maternal separation of rat pups results in abnormal cholinergic regulation of epithelial permeability Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G198 - G203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Ralph, D. Zocco, B. Bresnihan, O. FitzGerald, A. N. McEvoy, and E. P. Murphy A Role for Type 1{alpha} Corticotropin-Releasing Hormone Receptors in Mediating Local Changes in Chronically Inflamed Tissue Am. J. Pathol., March 1, 2007; 170(3): 1121 - 1133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markovic, N. Papadopoulou, T. Teli, H. Randeva, M. A. Levine, E. W. Hillhouse, and D. K. Grammatopoulos Differential Responses of Corticotropin-Releasing Hormone Receptor Type 1 Variants to Protein Kinase C Phosphorylation J. Pharmacol. Exp. Ther., December 1, 2006; 319(3): 1032 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, N. Prevete, I. Fiorentino, F. W. Rossi, S. Staibano, N. Montuori, P. Ragno, A. Longobardi, B. Liccardo, A. Genovese, et al. Expression and Functions of the Vascular Endothelial Growth Factors and Their Receptors in Human Basophils J. Immunol., November 15, 2006; 177(10): 7322 - 7331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Mastorakos, E. I Karoutsou, and M. Mizamtsidi Corticotropin releasing hormone and the immune/inflammatory response Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S77 - S84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Zoumakis, D K Grammatopoulos, and G P Chrousos Corticotropin-releasing hormone receptor antagonists Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S85 - S91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Donelan, W. Boucher, N. Papadopoulou, M. Lytinas, D. Papaliodis, P. Dobner, and T. C. Theoharides Corticotropin-releasing hormone induces skin vascular permeability through a neurotensin-dependent process PNAS, May 16, 2006; 103(20): 7759 - 7764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. W. Hillhouse and D. K. Grammatopoulos The Molecular Mechanisms Underlying the Regulation of the Biological Activity of Corticotropin-Releasing Hormone Receptors: Implications for Physiology and Pathophysiology Endocr. Rev., May 1, 2006; 27(3): 260 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cao, C. L. Cetrulo, and T. C. Theoharides Corticotropin-Releasing Hormone Induces Vascular Endothelial Growth Factor Release from Human Mast Cells via the cAMP/Protein Kinase A/p38 Mitogen-Activated Protein Kinase Pathway Mol. Pharmacol., March 1, 2006; 69(3): 998 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Buret How Stress Induces Intestinal Hypersensitivity Am. J. Pathol., January 1, 2006; 168(1): 3 - 5. [Full Text] [PDF]
|
|
|
|
|
|
P.-C. Yang, J. Jury, J. D. Soderholm, P. M. Sherman, D. M. McKay, and M. H. Perdue Chronic Psychological Stress in Rats Induces Intestinal Sensitization to Luminal Antigens Am. J. Pathol., January 1, 2006; 168(1): 104 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G Papadopoulou, L. Oleson, D. Kempuraj, J. Donelan, C. L Cetrulo, and T. C Theoharides Regulation of corticotropin-releasing hormone receptor-2 expression in human cord blood-derived cultured mast cells J. Mol. Endocrinol., December 1, 2005; 35(3): R1 - R8. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
