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Intrinsic Resistance of Hepatocytes to Complement-Mediated Injury¹

Cody A. Koch^2,*,, Akiyoshi Kanazawa^2,*, Ryuta Nishitai^*, Bruce E. Knudsen^*, Kiyoshi Ogata^*, Timothy B. Plummer^*, Kim Butters^* and Jeffrey L. Platt^3,*,,,

^* Transplantation Biology and the Departments of Immunology, Surgery, and Pediatrics, Mayo Clinic College of Medicine, Rochester, MN 55905

When activated on or in the vicinity of cells, complement usually causes loss of function and sometimes cell death. Yet the liver, which produces large amounts of complement proteins, clears activators of complement and activated complexes from portal blood without obvious injury or impaired function. We asked whether and to what extent hepatocytes resist injury and loss of function mediated by exposure to complement. Using cells isolated from porcine livers as a model system, we found that, in contrast to endothelial cells, hepatocytes profoundly resist complement-mediated lysis and exhibit normal synthetic and conjugative functions when complement is activated on their surface. The resistance of hepatocytes to complement-mediated injury was not a function of cell surface control of the complement cascade but rather an intrinsic resistance of the cells dependent on the PI3K/Akt pathway. The resistance of hepatocytes to complement might be exploited in developing approaches to the treatment of hepatic failure or more broadly to the treatment of complement-mediated disease.

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				S. M. Black, J. F. Grehan, A. L. Rivard, B. A. Benson, A. E. Wahner, A. E. Koch, B. K. Levay-Young, and A. P. Dalmasso Porcine Endothelial Cells and Iliac Arteries Transduced with AdenoIL-4 Are Intrinsically Protected, through Akt Activation, against Immediate Injury Caused by Human Complement J. Immunol., November 15, 2006; 177(10): 7355 - 7363. [Abstract] [Full Text] [PDF]