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CD4⁺CD25⁺ Regulatory T Cells Restrain Pathogenic Responses during Leishmania amazonensis Infection¹

Jiaxiang Ji^2,*, Joseph Masterson^*, Jiaren Sun^* and Lynn Soong^2,3,*,

Departments of^* Microbiology and Immunology and Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555

Although activation of CD4⁺ T cells mediates pathogenesis in Leishmania amazonensis (La)-infected mice, these susceptible mice do not develop a polarized Th2 response, suggesting a unique mechanism of disease susceptibility. To understand how Th cell activities are regulated, we examined the frequency and phenotypes of regulatory T (Treg) cells. At 1–3 wk of infection, relatively high percentages of CD4⁺CD25⁺CD86⁺ T cells, as well as high levels of FoxP3, TGF-1, and IL-10RI transcripts, were detected in the skin and draining lymph nodes, indicating local accumulation of Treg cells. Lesion-derived, IL-10-producing CD4⁺CD25⁺ cells effectively suppressed proliferation and cytokine (IL-2 and IFN-) production of CD4⁺CD25^– effector cells. Adoptive transfer of lesion-derived CD4⁺CD25⁺ cells to syngeneic, naive C57BL/6 mice before infection significantly reduced disease development. To further validate the beneficial role of Treg cells in La infection, we adoptively transferred CD25⁺ T cell-depleted splenocytes (derived from naive mice) into RAG1^–/– mice. This transfer rendered RAG1^–/– mice more susceptible to La infection than the mice receiving control splenocytes. The beneficial effect of Treg cells was transitory and correlated with decreased activation of IFN--producing effector T cells. This study uncovers an intriguing role of Treg cells in restraining pathogenic responses during nonhealing Leishmania infection and emphasizes a balance between Treg and Th1-like effector cells in determining the outcome of New World cutaneous leishmaniasis.

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