The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wen, L.
Right arrow Articles by Hayakawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wen, L.
Right arrow Articles by Hayakawa, K.
The Journal of Immunology, 2005, 174: 6918-6926.
Copyright © 2005 by The American Association of Immunologists

Association of B-1 B Cells with Follicular Dendritic Cells in Spleen1

Lijun Wen, Susan A. Shinton, Richard R. Hardy and Kyoko Hayakawa2

Fox Chase Cancer Center, Philadelphia, PA 19111

Although CD5+ B-1 B cells have been recognized as an infrequent B cell subset in mice for many years, attempts to identify their histologic location in normal mouse spleen have proven difficult due to both their paucity and low level expression of CD5. In this study we have studied VH11/DH/JH gene-targeted mice, VH11t, that develop elevated numbers of CD5+ VH11/Vk9 B cells with an anti-phosphatidylcholine (anti-PtC) autoreactive specificity, allowing B-1 B cell detection by anti-PtC Id-specific Abs in spleen section staining. Using this approach we found that anti-PtC B-1 cells first appear within the white pulp in neonates, expand in association with follicular dendritic cells (FDC), and localize more centrally than other (non-B-1) IgDhigh follicular B cells in adults. Among neonatal B cells, CD5+ B-1 cells in both normal and VH11t mouse spleen and peritoneal cavity express the highest levels of CXCR5, which is important for FDC development. Injection of purified spleen or peritoneal B-1 cells into RAG knockout mice resulted in B-1 cell follicle formation in spleen, inducing FDC development and plasma cell generation. These results indicate that B-1 B cells are the first B cells to express fully mature levels of CXCR5, thereby promoting the development of FDC.




This article has been cited by other articles:


Home page
 BloodHome page
T. Murakami, X. Chen, K. Hase, A. Sakamoto, C. Nishigaki, and H. Ohno
Splenic CD19 CD35+B220+ cells function as an inducer of follicular dendritic cell network formation
Blood, August 15, 2007; 110(4): 1215 - 1224.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Shimizu, T. Kawahara, F. Haspot, P. D. Bardwell, M. C. Carroll, and M. Sykes
B-cell extrinsic CR1/CR2 promotes natural antibody production and tolerance induction of anti-{alpha}GAL-producing B-1 cells
Blood, February 15, 2007; 109(4): 1773 - 1781.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S.-a. Ha, M. Tsuji, K. Suzuki, B. Meek, N. Yasuda, T. Kaisho, and S. Fagarasan
Regulation of B1 cell migration by signals through Toll-like receptors
J. Exp. Med., October 30, 2006; 203(11): 2541 - 2550.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.