The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Omatsu, Y.
Right arrow Articles by Inaba, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Omatsu, Y.
Right arrow Articles by Inaba, K.
The Journal of Immunology, 2005, 174: 6657-6662.
Copyright © 2005 by The American Association of Immunologists

Development of Murine Plasmacytoid Dendritic Cells Defined by Increased Expression of an Inhibitory NK Receptor, Ly49Q1

Yoshiki Omatsu*, Tomonori Iyoda*, Yukino Kimura*, Akiko Maki{dagger}, Masaki Ishimori*, Noriko Toyama-Sorimachi{dagger} and Kayo Inaba2,*

* Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto, Japan; and {dagger} Department of Gastroenterology, Research Institute, International Medical Center of Japan, Toyama, Tokyo, Japan

Plasmacytoid dendritic cells (PDCs) are defined in mice by a unique combination of markers: CD11c, B220, and Ly6C/G. We have reported previously that PDCs express Ly49Q, a lectin-type killer cell inhibitory receptor. We now find that different expression levels of Ly49Q define sequential developmental stages of PDCs in bone marrow. Although PDCs in spleen and lymph nodes express high levels of Ly49Q, a significant portion of CD11c+B220+ PDCs in bone marrow lack Ly49Q, as well as the CD4 and MHC II. Purified Ly49Q– marrow PDCs spontaneously up-regulate Ly49Q after overnight culture without cell proliferation and acquire most features of typical PDCs in spleen. When exposed to TLR ligands, such as CpG-oligodeoxynucleotide and hemagglutinating virus of Japan (Sendai virus), Ly49Q– PDCs increase CD86 and MHC class II expression but produce less IFN-{alpha}{beta}, IL-6, and IL-12p70 than Ly49Q+ PDCs, although they are able to produce comparable amounts of TNF-{alpha}. However, interestingly, Ly49Q– PDCs do not produce TNF-{alpha} in response to the TLR2 ligand, Pam3SCK4, whereas Ly49Q+ PDCs did. Therefore, Ly49Q is a new marker to identify a precursor form of PDCs that participates in innate immunity.




This article has been cited by other articles:


Home page
 BloodHome page
B. Fancke, M. Suter, H. Hochrein, and M. O'Keeffe
M-CSF: a novel plasmacytoid and conventional dendritic cell poietin
Blood, January 1, 2008; 111(1): 150 - 159.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Contractor, J. Louten, L. Kim, C. A. Biron, and B. L. Kelsall
Cutting Edge: Peyer's Patch Plasmacytoid Dendritic Cells (pDCs) Produce Low Levels of Type I Interferons: Possible Role for IL-10, TGFbeta, and Prostaglandin E2 in Conditioning a Unique Mucosal pDC Phenotype
J. Immunol., September 1, 2007; 179(5): 2690 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Vremec, M. O'Keeffe, H. Hochrein, M. Fuchsberger, I. Caminschi, M. Lahoud, and K. Shortman
Production of interferons by dendritic cells, plasmacytoid cells, natural killer cells, and interferon-producing killer dendritic cells
Blood, February 1, 2007; 109(3): 1165 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Allman, M. Dalod, C. Asselin-Paturel, T. Delale, S. H. Robbins, G. Trinchieri, C. A. Biron, P. Kastner, and S. Chan
Ikaros is required for plasmacytoid dendritic cell differentiation
Blood, December 15, 2006; 108(13): 4025 - 4034.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Lund, M. M. Linehan, N. Iijima, and A. Iwasaki
Cutting Edge: Plasmacytoid Dendritic Cells Provide Innate Immune Protection against Mucosal Viral Infection In Situ
J. Immunol., December 1, 2006; 177(11): 7510 - 7514.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Gays, J. G. Aust, D. M. Reid, J. Falconer, N. Toyama-Sorimachi, P. R. Taylor, and C. G. Brooks
Ly49B Is Expressed on Multiple Subpopulations of Myeloid Cells
J. Immunol., November 1, 2006; 177(9): 5840 - 5851.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Blasius, E. Giurisato, M. Cella, R. D. Schreiber, A. S. Shaw, and M. Colonna
Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naive Mouse, but a Promiscuous Cell Surface Antigen following IFN Stimulation.
J. Immunol., September 1, 2006; 177(5): 3260 - 3265.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Zhang, A. Raper, N. Sugita, R. Hingorani, M. Salio, M. J. Palmowski, V. Cerundolo, and P. R. Crocker
Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors
Blood, May 1, 2006; 107(9): 3600 - 3608.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Huang, F. Rezzoug, H. Xu, P. M. Chilton, C. L. Schanie, I. Fugier-Vivier, and S. T. Ildstad
NK Cells Play a Critical Role in the Regulation of Class I-Deficient Hemopoietic Stem Cell Engraftment: Evidence for NK Tolerance Correlates with Receptor Editing
J. Immunol., September 15, 2005; 175(6): 3753 - 3761.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.