HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Toliver-Kinsky, T. E. Articles by Sherwood, E. R. Search for Related Content PubMed PubMed Citation Articles by Toliver-Kinsky, T. E. Articles by Sherwood, E. R.

Enhancement of Dendritic Cell Production by Fms-Like Tyrosine Kinase-3 Ligand Increases the Resistance of Mice to a Burn Wound Infection¹

Tracy E. Toliver-Kinsky^2,*,, Weihua Cui^*, Erle D. Murphey^*,, Chengyie Lin^* and Edward R. Sherwood^*,

^* Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX 77555; and Shriners Hospitals for Children, Galveston Burn Unit, Galveston, TX 77550

Fms-like tyrosine kinase-3 ligand (Flt3L) is a hemopoietic cytokine that stimulates the production of dendritic cells. This study evaluated the ability of Flt3L-enhanced dendritic cell production to increase the resistance of mice to a burn wound infection with Pseudomonas aeruginosa, a common source of infections in burn patients that have impaired immunity and are susceptible to opportunistic microorganisms. Treatment of mice with Flt3L for 5 days caused a significant increase in dendritic cell numbers in the spleen and significantly increased survival upon a subsequent burn wound infection. Improved survival in Flt3L-treated mice was associated with limited bacterial growth and spread within the burn wounds and a decrease in systemic dissemination of P. aeruginosa. Resistance to burn wound infection could also be conferred to recipient mice by the adoptive transfer of dendritic cells that had been isolated from spleens of Flt3L-treated mice. Adoptive transfer of the same number of splenic dendritic cells from nontreated mice did not confer resistance to burn wound infection. These data indicate that Flt3L can increase the resistance of mice to a P. aeruginosa burn wound infection through both stimulation of dendritic cell production and enhancement of dendritic cell function.

This article has been cited by other articles:

				S. Fujimi, P. H. Lapchak, Y. Zang, M. P. MacConmara, A. A. Maung, A. J. Delisle, J. A. Mannick, and J. A. Lederer Murine dendritic cell antigen-presenting cell function is not altered by burn injury J. Leukoc. Biol., May 1, 2009; 85(5): 862 - 870. [Abstract] [Full Text] [PDF]

				F. Pene, B. Zuber, E. Courtine, C. Rousseau, F. Ouaaz, J. Toubiana, A. Tazi, J.-P. Mira, and J.-D. Chiche Dendritic Cells Modulate Lung Response to Pseudomonas aeruginosa in a Murine Model of Sepsis-Induced Immune Dysfunction J. Immunol., December 15, 2008; 181(12): 8513 - 8520. [Abstract] [Full Text] [PDF]

				J. Bohannon, W. Cui, R. Cox, R. Przkora, E. Sherwood, and T. Toliver-Kinsky Prophylactic Treatment with Fms-Like Tyrosine Kinase-3 Ligand after Burn Injury Enhances Global Immune Responses to Infection J. Immunol., March 1, 2008; 180(5): 3038 - 3048. [Abstract] [Full Text] [PDF]

				X. Li, E. J. Kovacs, M. G. Schwacha, I. H. Chaudry, and M. A. Choudhry Acute alcohol intoxication increases interleukin-18-mediated neutrophil infiltration and lung inflammation following burn injury in rats Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1193 - L1201. [Abstract] [Full Text] [PDF]