| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
* Mucosal Immunology Laboratory, Massachusetts General Hospital, Charlestown, MA 02129;
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02155; and
Cardiff University, School of Pharmacy, Cardiff, Wales
Lung inflammation resulting from bacterial infection of the respiratory mucosal surface in diseases such as cystic fibrosis and pneumonia contributes significantly to the pathology. A major consequence of the inflammatory response is the recruitment and accumulation of polymorphonuclear cells (PMNs) at the infection site. It is currently unclear what bacterial factors trigger this response and exactly how PMNs are directed across the epithelial barrier to the airway lumen. An in vitro model consisting of human PMNs and alveolar epithelial cells (A549) grown on inverted Transwell filters was used to determine whether bacteria are capable of inducing PMN migration across these epithelial barriers. A variety of lung pathogenic bacteria, including Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa are indeed capable of inducing PMN migration across A549 monolayers. This phenomenon is not mediated by LPS, but requires live bacteria infecting the apical surface. Bacterial interaction with the apical surface of A549 monolayers results in activation of epithelial responses, including the phosphorylation of ERK1/2 and secretion of the PMN chemokine IL-8. However, secretion of IL-8 in response to bacterial infection is neither necessary nor sufficient to mediate PMN transepithelial migration. Instead, PMN transepithelial migration is mediated by the eicosanoid hepoxilin A3, which is a PMN chemoattractant secreted by A549 cells in response to bacterial infection in a protein kinase C-dependent manner. These data suggest that bacterial-induced hepoxilin A3 secretion may represent a previously unrecognized inflammatory mechanism occurring within the lung epithelium during bacterial infections.
This article has been cited by other articles:
|
|
 |
|
  J. Chun and A. Prince Ca2+ signaling in airway epithelial cells facilitates leukocyte recruitment and transepithelial migration J. Leukoc. Biol., November 1, 2009; 86(5): 1135 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, T. Rui, M. Yang, F. Valiyeva, and P. R. Kvietys Alveolar Macrophages from Septic Mice Promote Polymorphonuclear Leukocyte Transendothelial Migration via an Endothelial Cell Src Kinase/NADPH Oxidase Pathway J. Immunol., December 15, 2008; 181(12): 8735 - 8744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pazos, D. Siccardi, K. L. Mumy, J. D. Bien, S. Louie, H. N. Shi, K. Gronert, R. J. Mrsny, and B. A. McCormick Multidrug Resistance-Associated Transporter 2 Regulates Mucosal Inflammation by Facilitating the Synthesis of Hepoxilin A3 J. Immunol., December 1, 2008; 181(11): 8044 - 8052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Mumy, J. D. Bien, M. A. Pazos, K. Gronert, B. P. Hurley, and B. A. McCormick Distinct Isoforms of Phospholipase A2 Mediate the Ability of Salmonella enterica Serotype Typhimurium and Shigella flexneri To Induce the Transepithelial Migration of Neutrophils Infect. Immun., August 1, 2008; 76(8): 3614 - 3627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K J Mackerness, G R Jenkins, A Bush, and P J Jose Characterisation of the range of neutrophil stimulating mediators in cystic fibrosis sputum Thorax, July 1, 2008; 63(7): 614 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Swee, C. L. Wilson, Y. Wang, J. K. McGuire, and W. C. Parks Matrix metalloproteinase-7 (matrilysin) controls neutrophil egress by generating chemokine gradients J. Leukoc. Biol., June 1, 2008; 83(6): 1404 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-A. Raquil, N. Anceriz, P. Rouleau, and P. A. Tessier Blockade of Antimicrobial Proteins S100A8 and S100A9 Inhibits Phagocyte Migration to the Alveoli in Streptococcal Pneumonia J. Immunol., March 1, 2008; 180(5): 3366 - 3374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Mumy, X. Chen, C. P. Kelly, and B. A. McCormick Saccharomyces boulardii interferes with Shigella pathogenesis by postinvasion signaling events Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G599 - G609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Zurawski, K. L. Mumy, L. Badea, J. A. Prentice, E. L. Hartland, B. A. McCormick, and A. T. Maurelli The NleE/OspZ Family of Effector Proteins Is Required for Polymorphonuclear Transepithelial Migration, a Characteristic Shared by Enteropathogenic Escherichia coli and Shigella flexneri Infections Infect. Immun., January 1, 2008; 76(1): 369 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Hurley, N. L. Williams, and B. A. McCormick Involvement of phospholipase A2 in Pseudomonas aeruginosa-mediated PMN transepithelial migration Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L703 - L709. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
