| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada; and
Department of Biological Sciences, Imperial College, London, United Kingdom
Killer cell Ig-like receptors (KIRs) are MHC class I-specific receptors expressed in NK and T lymphocytes. KIR antagonism of activation signals occurs at the immune synapse between the effector and target cells. The processes that regulate clustering of KIR are not well defined. We have expressed KIR-GFP receptor chimeras in two human NK-like lines, YTS and NK92. In this study, we show that the frequency of KIR enrichment at the synapse was decreased for a KIR that lacks a portion of the cytoplasmic tail. Strikingly, blocking actin polymerization with a high dose of cytochalasin D also substantially decreased clustering of KIR as well as KIR-induced clustering of HLA-C-GFP in target cells. However, the effect of inhibiting actin polymerization was only clearly evident at the earlier time points after cell mixing, and eventually clustering of KIR and HLA-C occurred independently of actin remodeling. Although treatment with anti-LFA-1 also decreased conjugate formation, the frequency of KIR clustering remained normal within the population of conjugates that did form, suggesting that the effect of cytochalasin D is not solely through LFA-1. Collectively, these data suggest that the actin cytoskeleton and the cytoplasmic tail of KIR regulate the efficiency by which KIR accumulates at inhibitory NK cell synapses.
This article has been cited by other articles:
|
|
 |
|
  O. Hershkovitz, A. Zilka, A. Bar-Ilan, S. Abutbul, A. Davidson, M. Mazzon, B. M. Kummerer, A. Monsoengo, M. Jacobs, and A. Porgador Dengue Virus Replicon Expressing the Nonstructural Proteins Suffices To Enhance Membrane Expression of HLA Class I and Inhibit Lysis by Human NK Cells J. Virol., August 1, 2008; 82(15): 7666 - 7676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Markasz, G. Stuber, B. Vanherberghen, E. Flaberg, E. Olah, E. Carbone, S. Eksborg, E. Klein, H. Skribek, and L. Szekely Effect of frequently used chemotherapeutic drugs on the cytotoxic activity of human natural killer cells Mol. Cancer Ther., February 1, 2007; 6(2): 644 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Almeida and D. M. Davis Segregation of HLA-C from ICAM-1 at NK Cell Immune Synapses Is Controlled by Its Cell Surface Density J. Immunol., November 15, 2006; 177(10): 6904 - 6910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Treanor, P. M.P. Lanigan, S. Kumar, C. Dunsby, I. Munro, E. Auksorius, F. J. Culley, M. A. Purbhoo, D. Phillips, M. A.A. Neil, et al. Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses J. Cell Biol., July 3, 2006; 174(1): 153 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Henel, K. Singh, D. Cui, S. Pryshchep, W.-W. Lee, C. M. Weyand, and J. J. Goronzy Uncoupling of T-cell effector functions by inhibitory killer immunoglobulin-like receptors Blood, June 1, 2006; 107(11): 4449 - 4457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ebata, Y. Shimizu, Y. Nakayama, M. Minemura, J. Murakami, T. Kato, S. Yasumura, T. Takahara, T. Sugiyama, and S. Saito Immature NK Cells Suppress Dendritic Cell Functions during the Development of Leukemia in a Mouse Model J. Immunol., April 1, 2006; 176(7): 4113 - 4124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Kirwan and D. N. Burshtyn Killer Cell Ig-Like Receptor-Dependent Signaling by Ig-Like Transcript 2 (ILT2/CD85j/LILRB1/LIR-1) J. Immunol., October 15, 2005; 175(8): 5006 - 5015. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
