| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Microbiology and
Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032
The transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is expressed in some differentiated cells and is required for terminal differentiation of B cells. To facilitate identification of Blimp-1 target genes, we have determined the optimal DNA recognition sequence for Blimp-1. The consensus is very similar to a subset of sites recognized by IFN regulatory factors (IRFs) that contain the sequence GAAAG. By binding competition and determination of equilibrium dissociation constants, we show that Blimp-1, IRF-1, and IRF-2 have similar binding affinities for functionally important regulatory sites containing this sequence. However, Blimp-1 does not bind to all IRF sites, and specifically does not recognize IRF-4/PU.1 or IRF-8 sites lacking the GAAAG sequence. Chromatin immunoprecipitation studies showed that Blimp-1, IRF-1, and IRF-2 all bind the IFN-
promoter in vivo, as predicted by the in vitro binding parameters, and in cotransfections Blimp-1 inhibits IRF-1-dependent activation of the IFN- promoter. Thus, our data suggest that Blimp-1 competes in vivo with a subset of IRF proteins and help predict the sites and IRF family members that may be affected.
This article has been cited by other articles:
|
|
 |
|
  S.-T. Su, H.-Y. Ying, Y.-K. Chiu, F.-R. Lin, M.-Y. Chen, and K.-I Lin Involvement of Histone Demethylase LSD1 in Blimp-1-Mediated Gene Repression during Plasma Cell Differentiation Mol. Cell. Biol., March 15, 2009; 29(6): 1421 - 1431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Lord, D. Savitsky, R. Sitcheran, K. Calame, J. R. Wright, J. P.-Y. Ting, and K. L. Williams Blimp-1/PRDM1 Mediates Transcriptional Suppression of the NLR Gene NLRP12/Monarch-1 J. Immunol., March 1, 2009; 182(5): 2948 - 2958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Smith and E. H. Davidson Gene Networks in Development and Evolution Special Feature Sackler Colloquium: Gene regulatory network subcircuit controlling a dynamic spatial pattern of signaling in the sea urchin embryo PNAS, December 23, 2008; 105(51): 20089 - 20094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, N. van Panhuys, J. Hu-Li, S. Kim, G. Le Gros, and B. Min Blimp-1 Induced by IL-4 Plays a Critical Role in Suppressing IL-2 Production in Activated CD4 T Cells J. Immunol., October 15, 2008; 181(8): 5249 - 5256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cimmino, G. A. Martins, J. Liao, E. Magnusdottir, G. Grunig, R. K. Perez, and K. L. Calame Blimp-1 Attenuates Th1 Differentiation by Repression of ifng, tbx21, and bcl6 Gene Expression J. Immunol., August 15, 2008; 181(4): 2338 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurimoto, Y. Yabuta, Y. Ohinata, M. Shigeta, K. Yamanaka, and M. Saitou Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice Genes & Dev., June 15, 2008; 22(12): 1617 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Doody, S. Stephenson, C. McManamy, and R. M. Tooze PRDM1/BLIMP-1 Modulates IFN-{gamma}-Dependent Control of the MHC Class I Antigen-Processing and Peptide-Loading Pathway J. Immunol., December 1, 2007; 179(11): 7614 - 7623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Magnusdottir, S. Kalachikov, K. Mizukoshi, D. Savitsky, A. Ishida-Yamamoto, A. A. Panteleyev, and K. Calame Epidermal terminal differentiation depends on B lymphocyte-induced maturation protein-1 PNAS, September 18, 2007; 104(38): 14988 - 14993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhao, E. N. Cha, C. Lee, C. Y. Park, and C. Schindler Stat2-Dependent Regulation of MHC Class II Expression J. Immunol., July 1, 2007; 179(1): 463 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Tooze, S. Stephenson, and G. M. Doody Repression of IFN-{gamma} Induction of Class II Transactivator: A Role for PRDM1/Blimp-1 in Regulation of Cytokine Signaling J. Immunol., October 1, 2006; 177(7): 4584 - 4593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-I Lin, Y.-Y. Kao, H.-K. Kuo, W.-B. Yang, A. Chou, H.-H. Lin, A. L. Yu, and C.-H. Wong Reishi Polysaccharides Induce Immunoglobulin Production through the TLR4/TLR2-mediated Induction of Transcription Factor Blimp-1 J. Biol. Chem., August 25, 2006; 281(34): 24111 - 24123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Coro, W. L. W. Chang, and N. Baumgarth Type I IFN Receptor Signals Directly Stimulate Local B Cells Early following Influenza Virus Infection J. Immunol., April 1, 2006; 176(7): 4343 - 4351. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
