| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
,¶,#,,¶,||
* Laboratory of Immunology,
Département de Microbiologie et d’Immunologie and
Centre de Recherche du Centre Hospitalier de l’Université de Montréal, and
Département de Médecine, Université de Montréal, Montreal, Canada;
¶
Department of Microbiology and Immunology and
||
Faculty of Medicine, Division of Experimental Medicine, McGill University, Montreal, Canada;
#
Guy-Bernier Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada;
**
Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322; and
Ontario Cancer Institute, Department of Medicine, University of Toronto, Toronto, Canada
Caspases play a central role in T lymphocyte activation and death. We have demonstrated previously that caspase-3, an effector molecule for activation-induced cell death (AICD), is processed following T cell activation in the absence of apoptosis. We report in this study that caspase-3 mRNA levels were selectively increased in peripheral T cells, following Ag receptor-mediated activation. The up-regulation of caspase-3 mRNA was confined to cells in the early phases of the cell cycle (G0/G1) and was independent of IL-2 signaling. This increase led to the renewal of procaspase-3 as evidenced by a 6-fold up-regulation of the zymogen in nonapoptotic stimulated T cells. The increase of mRNA levels and of both the zymogen and the cleaved forms of caspase-3 was observed in in vivo stimulated Ag-specific effector, but not memory T cells, correlating with the enhanced susceptibility of effector T cells to AICD. Furthermore, we confirm that caspase-3 levels directly influence the sensitivity of activated T cells to apoptosis, as shown using T lymphocytes isolated from caspase-3 heterozygous and knockout mice. These findings indicate that the selective up-regulation of caspase-3 transcription is required to maintain the cytoplasmic levels of this protease, which control AICD and T cell homeostasis.
This article has been cited by other articles:
|
|
 |
|
  J. A. Carrero, H. Vivanco-Cid, and E. R. Unanue Granzymes Drive a Rapid Listeriolysin O-Induced T Cell Apoptosis J. Immunol., July 15, 2008; 181(2): 1365 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. T. Tan, Y. Sun, J. Song, Y. Chen, T. G. Krontiris, and L. K. Durrin SUMO Conjugation to the Matrix Attachment Region-binding Protein, Special AT-rich Sequence-binding Protein-1 (SATB1), Targets SATB1 to Promyelocytic Nuclear Bodies Where It Undergoes Caspase Cleavage J. Biol. Chem., June 27, 2008; 283(26): 18124 - 18134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Genesca, T. Rourke, J. Li, K. Bost, B. Chohan, M. B. McChesney, and C. J. Miller Live Attenuated Lentivirus Infection Elicits Polyfunctional Simian Immunodeficiency Virus Gag-Specific CD8+ T Cells with Reduced Apoptotic Susceptibility in Rhesus Macaques that Control Virus Replication after Challenge with Pathogenic SIVmac239 J. Immunol., October 1, 2007; 179(7): 4732 - 4740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Maurer, M. Chiorazzi, and S. Shaham Timing of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene Development, April 1, 2007; 134(7): 1357 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kerstan, N. Armbruster, M. Leverkus, and T. Hunig Cyclosporin A Abolishes CD28-Mediated Resistance to CD95-Induced Apoptosis via Superinduction of Caspase-3 J. Immunol., December 1, 2006; 177(11): 7689 - 7697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Lacombe, M.-P. Hardy, J. Rooney, and N. Labrecque IL-7 Receptor Expression Levels Do Not Identify CD8+ Memory T Lymphocyte Precursors following Peptide Immunization J. Immunol., October 1, 2005; 175(7): 4400 - 4407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-F. Flach, S. Lange, E. Jennische, I. Lonnroth, and J. Holmgren Cholera Toxin Induces a Transient Depletion of CD8+ Intraepithelial Lymphocytes in the Rat Small Intestine as Detected by Microarray and Immunohistochemistry Infect. Immun., September 1, 2005; 73(9): 5595 - 5602. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
