| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Allergy and Rheumatology and
Bioregulatory Function, University of Tokyo Graduate School of Medicine, Tokyo, Japan;
Department of Pediatrics, National Sagamihara Hospital, Kanagawa; and
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan
During allergic reactions, basophils migrate from the blood compartment to inflammatory sites, where they act as effector cells in concert with eosinophils. Because transendothelial migration (TEM) represents an essential step for extravasation of cells, for the first time we have studied basophil TEM using HUVEC. Treatment of HUVEC with IL-1
significantly enhanced basophil TEM, which was further potentiated by the presence of a CCR3-specific ligand, eotaxin/CCL11. In addition to CCR3 ligands, MCP-1/CCL2 was also active on basophil TEM. Although stromal cell-derived factor-1/CXCL12, a CXCR4 ligand, failed to induce TEM in freshly isolated basophils, it caused strong TEM in 24-h cultured cells. IL-3 enhanced basophil TEM by increasing the chemokinetic response. Spontaneous TEM across activated HUVEC was inhibited by treatment of cells with anti-CD18 mAb, but not with anti-CD29 mAb, and also by treatment of HUVEC with anti-ICAM-1 mAb. Anti-VCAM-1 mAb alone failed to inhibit TEM, but showed an additive inhibitory effect in combination with anti-ICAM-1 mAb. In contrast, eotaxin- and IL-3-mediated TEM was significantly inhibited by anti-CD29 mAb as well as anti-CD18 mAb. These results indicate that 2 integrins play the primary role in basophil TEM, but 1 integrins are also involved, especially in TEM of cytokine/chemokine-stimulated basophils. In conclusion, the regulatory profile of basophil TEM is very similar to that reported for eosinophils. Our results thus support the previous argument for a close relationship between basophils and eosinophils and suggest that the in vivo kinetics of these two cell types are similar.
This article has been cited by other articles:
|
|
 |
|
  D. Uchida, T. Onoue, Y. Tomizuka, N. M. Begum, Y. Miwa, H. Yoshida, and M. Sato Involvement of an Autocrine Stromal Cell Derived Factor-1/CXCR4 System on the Distant Metastasis of Human Oral Squamous Cell Carcinoma Mol. Cancer Res., July 1, 2007; 5(7): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Suzukawa, A. Komiya, M. Iikura, H. Nagase, C. Yoshimura-Uchiyama, H. Yamada, H. Kawasaki, K. Ohta, K. Matsushima, K. Hirai, et al. Trans-basement membrane migration of human basophils: role of matrix metalloproteinase-9 Int. Immunol., November 1, 2006; 18(11): 1575 - 1583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. K. Lim, M. M. Burdick, S. A. Hudson, F. B. Mustafa, K. Konstantopoulos, and B. S. Bochner Stimulation of Human Endothelium with IL-3 Induces Selective Basophil Accumulation In Vitro J. Immunol., May 1, 2006; 176(9): 5346 - 5353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suzukawa, K. Hirai, M. Iikura, H. Nagase, A. Komiya, C. Yoshimura-Uchiyama, H. Yamada, C. Ra, K. Ohta, K. Yamamoto, et al. IgE- and Fc{varepsilon}RI-mediated migration of human basophils Int. Immunol., September 1, 2005; 17(9): 1249 - 1255. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
