HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonnotte, B. Articles by Vile, R. G. Search for Related Content PubMed PubMed Citation Articles by Bonnotte, B. Articles by Vile, R. G.

MIP-3 Transfection into a Rodent Tumor Cell Line Increases Intratumoral Dendritic Cell Infiltration but Enhances (Facilitates) Tumor Growth and Decreases Immunogenicity¹

Bernard Bonnotte^*,, Marka Crittenden^*, Nicolas Larmonier, Michael Gough^* and Richard G. Vile^2,*

^* Molecular Medicine Program and Department of Immunology, Mayo Clinic, Rochester, MN 55905; and Institut National de la Santé et de la Recherché Médicale Unité 517, Faculty of Medicine, Dijon, France

Dendritic cells are powerful APCs for activation of specific antitumor T lymphocytes. To present tumor Ags efficiently, they have first to migrate to the tumor site, engulf Ag, and then process them. To attract immature DCs to the tumor site, we transfected tumor cells with MIP-3 which is strongly chemotactic for DCs. Surprisingly, MIP-3-transfected tumor cells grew faster than the mock-transfected tumor cells. Histological analysis and tumor dissociation confirmed that the MIP-3-transfected tumors contain three to four times more DCs than mock-transfected tumors. FACS analysis of the intratumor DCs showed that they were predominantly immature. Functional analysis showed that the alloreactivity mediated by these infiltrating MIP-3-transfected tumor DCs is strongly reduced. In conclusion, MIP-3 is an efficient chemokine for attracting DCs in vivo, but the high density of DCs in the tumor site injection is not a sufficient condition to induce an immune response. Furthermore, this attraction of immature DCs may always have an adverse effect by inducing a tolerance to the tumor cells.

This article has been cited by other articles:

				Y.-s. Wang, D. Li, H.-s. Shi, Y.-j. Wen, L. Yang, N. Xu, X.-c. Chen, X. Chen, P. Chen, J. Li, et al. Intratumoral Expression of Mature Human Neutrophil Peptide-1 Mediates Antitumor Immunity in Mice Clin. Cancer Res., November 15, 2009; 15(22): 6901 - 6911. [Abstract] [Full Text] [PDF]

				E. Ramakrishna, N. Woller, B. Mundt, S. Knocke, E. Gurlevik, M. Saborowski, N. Malek, M. P. Manns, T. Wirth, F. Kuhnel, et al. Antitumoral Immune Response by Recruitment and Expansion of Dendritic Cells in Tumors Infected with Telomerase-Dependent Oncolytic Viruses Cancer Res., February 15, 2009; 69(4): 1448 - 1458. [Abstract] [Full Text] [PDF]

				A. Nicolas, D. Cathelin, N. Larmonier, J. Fraszczak, P.-E. Puig, A. Bouchot, A. Bateman, E. Solary, and B. Bonnotte Dendritic Cells Trigger Tumor Cell Death by a Nitric Oxide-Dependent Mechanism J. Immunol., July 15, 2007; 179(2): 812 - 818. [Abstract] [Full Text] [PDF]

				A. Bergeron, F. El Hage, M. Kambouchner, D. Lecossier, and A. Tazi Characterisation of dendritic cell subsets in lung cancer micro-environments Eur. Respir. J., December 1, 2006; 28(6): 1170 - 1177. [Abstract] [Full Text] [PDF]