| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Medicine, Section of Gastroenterology, University of Chicago, Chicago, IL 60637
The leukocyte-specific integrin, LFA-1, plays a critical role in trafficking of T cells to both lymphoid and nonlymphoid tissues. However, the role of LFA-1 in T cell activation in vivo has been less well understood. Although there have been reports describing LFA-1-deficient T cell response defects in vivo, due to impaired migration to lymphoid structures and to sites of effector function in the absence of LFA-1, it has been difficult to assess whether T cells also have a specific activation defect in vivo. We examined the role of LFA-1 in CD4+ T cell activation in vivo by using a system that allows for segregation of the migration and activation defects through the adoptive transfer of LFA-1-deficient (CD18–/–) CD4+ T cells from DO11.10 Ag-specific TCR transgenic mice into wild-type BALB/c mice. We find that in addition to its role in trafficking to peripheral lymph nodes, LFA-1 is required for optimal CD4+ T cell priming in vivo upon s.c. immunization. CD18–/– DO11.10 CD4+ T cells primed in the lymph nodes demonstrate defects in IL-2 and IFN-
production. In addition, recipient mice adoptively transferred with CD18–/– DO11.10 CD4+ T cells demonstrate a defect in OVA-specific IgG2a production after s.c. immunization. The defect in priming of CD18–/– CD4+ T cells persists even in the presence of proliferating CD18+/– CD4+ T cells and in lymphoid structures to which there is no migration defect. Taken together, these results demonstrate that LFA-1 is required for optimal CD4+ T cell priming in vivo.
This article has been cited by other articles:
|
|
 |
|
  Y. Wang, K. Shibuya, Y. Yamashita, J. Shirakawa, K. Shibata, H. Kai, T. Yokosuka, T. Saito, S.-i. Honda, S. Tahara-Hanaoka, et al. LFA-1 decreases the antigen dose for T cell activation in vivo Int. Immunol., September 1, 2008; 20(9): 1119 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Mueller, M. S. Thomas, B. J. Burbach, E. J. Peterson, and Y. Shimizu Adhesion and Degranulation-Promoting Adapter Protein (ADAP) Positively Regulates T Cell Sensitivity to Antigen and T Cell Survival J. Immunol., September 15, 2007; 179(6): 3559 - 3569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Graf, T. Bushnell, and J. Miller LFA-1-Mediated T Cell Costimulation through Increased Localization of TCR/Class II Complexes to the Central Supramolecular Activation Cluster and Exclusion of CD45 from the Immunological Synapse J. Immunol., August 1, 2007; 179(3): 1616 - 1624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Castriconi, A. Dondero, C. Cantoni, M. Della Chiesa, C. Prato, M. Nanni, M. Fiorini, L. Notarangelo, S. Parolini, L. Moretta, et al. Functional characterization of natural killer cells in type I leukocyte adhesion deficiency Blood, June 1, 2007; 109(11): 4873 - 4881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marski, A. L. Ye, and C. Abraham CD18 Is Required for Intestinal T Cell Responses at Multiple Immune Checkpoints J. Immunol., February 15, 2007; 178(4): 2104 - 2112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Varga, S. Balkow, M. K. Wild, A. Stadtbaeumer, M. Krummen, T. Rothoeft, T. Higuchi, S. Beissert, K. Wethmar, K. Scharffetter-Kochanek, et al. Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation Blood, January 15, 2007; 109(2): 661 - 669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. N. Simonson, S. J. Franco, and A. Huttenlocher Talin1 Regulates TCR-Mediated LFA-1 Function J. Immunol., December 1, 2006; 177(11): 7707 - 7714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghosh, A. A. Chackerian, C. M. Parker, C. M. Ballantyne, and S. M. Behar The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J. Immunol., April 15, 2006; 176(8): 4914 - 4922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marski, S. Kandula, J. R. Turner, and C. Abraham CD18 Is Required for Optimal Development and Function of CD4+CD25+ T Regulatory Cells J. Immunol., December 15, 2005; 175(12): 7889 - 7897. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
