| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B Activation through Covalent Modification of Reduced Cysteine 62 of p501
* Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China;
Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang, Liaoning, China;
State Key Laboratory of Bioorganic & Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China; and
Vascular Biology Center and Division of Hematology, Oncology and Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455
NF-
B is a central transcriptional factor and a pleiotropic regulator of many genes involved in immunological responses. During the screening of a plant extract library of traditional Chinese herbal medicines, we found that NF-B activity was potently inhibited by andrographolide (Andro), an abundant component of the plant Andrographis that has been commonly used as a folk remedy for alleviation of inflammatory disorders in Asia for millennia. Mechanistically, it formed a covalent adduct with reduced cysteine (62) of p50, thus blocking the binding of NF-B oligonucleotide to nuclear proteins. Andro suppressed the activation of NF-B in stimulated endothelial cells, which reduced the expression of cell adhesion molecule E-selectin and prevented E-selectin-mediated leukocyte adhesion under flow. It also abrogated the cytokine- and endotoxin-induced peritoneal deposition of neutrophils, attenuated septic shock, and prevented allergic lung inflammation in vivo. Notably, it had no suppressive effect on IB
degradation, p50 and p65 nuclear translocation, or cell growth rates. Our results thus reveal a unique pharmacological mechanism of Andro’s protective anti-inflammatory actions.
This article has been cited by other articles:
|
|
 |
|
  A. Varma, H. Padh, and N. Shrivastava Andrographolide: A New Plant-Derived Antineoplastic Entity on Horizon Evid. Based Complement. Altern. Med., September 14, 2009; (2009) nep135v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-W. Chao, Y.-H. Kuo, S.-L. Hsieh, and B.-F. Lin Inhibitory effects of ethyl acetate extract of Andrographis paniculata on NF-{kappa}B trans-activation activity and LPS-induced acute inflammation in mice Evid. Based Complement. Altern. Med., September 10, 2009; (2009) nep120v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Bao, S. Guan, C. Cheng, S. Wu, S. H. Wong, D. M. Kemeny, B. P. Leung, and W. S. F. Wong A Novel Antiinflammatory Role for Andrographolide in Asthma via Inhibition of the Nuclear Factor-{kappa}B Pathway Am. J. Respir. Crit. Care Med., April 15, 2009; 179(8): 657 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-D. Li, B.-Q. Ye, S.-X. Zheng, J.-T. Wang, J.-G. Wang, M. Chen, J.-G. Liu, X.-H. Pei, L.-J. Wang, Z.-X. Lin, et al. NF-{kappa}B Transcription Factor p50 Critically Regulates Tissue Factor in Deep Vein Thrombosis J. Biol. Chem., February 13, 2009; 284(7): 4473 - 4483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jiang, R. Goel, M. A. Iftekhar, R. Visaria, J. D. Belcher, G. M. Vercellotti, and J. C. Bischof Tumor necrosis factor-{alpha}-induced accentuation in cryoinjury: mechanisms in vitro and in vivo Mol. Cancer Ther., August 1, 2008; 7(8): 2547 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhou, G.-D. Lu, C.-S. Ong, C.-N. Ong, and H.-M. Shen Andrographolide sensitizes cancer cells to TRAIL-induced apoptosis via p53-mediated death receptor 4 up-regulation Mol. Cancer Ther., July 1, 2008; 7(7): 2170 - 2180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. H. Woo, M. M. Y. Waye, S. K. W. Tsui, S. T. W. Yeung, and C. H. K. Cheng Andrographolide Up-Regulates Cellular-Reduced Glutathione Level and Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury J. Pharmacol. Exp. Ther., April 1, 2008; 325(1): 226 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-P. Liang, C.-H. Lin, C.-D. Kuo, H.-P. Chao, and S.-L. Fu Suppression of v-Src Transformation by Andrographolide via Degradation of the v-Src Protein and Attenuation of the Erk Signaling Pathway J. Biol. Chem., February 22, 2008; 283(8): 5023 - 5033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, S. A. Mahmud, P. B. Bitterman, Y. Huo, and A. Slungaard Histone Deacetylase Inhibitors Suppress TF-{kappa}B-dependent Agonist-driven Tissue Factor Expression in Endothelial Cells and Monocytes J. Biol. Chem., September 28, 2007; 282(39): 28408 - 28418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lambert, J. Li, K. Jonscher, T.-C. Yang, P. Reigan, M. Quintana, J. Harvey, and B. M. Freed Acrolein Inhibits Cytokine Gene Expression by Alkylating Cysteine and Arginine Residues in the NF-{kappa}B1 DNA Binding Domain J. Biol. Chem., July 6, 2007; 282(27): 19666 - 19675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Wang, S. A. Mahmud, J. Nguyen, and A. Slungaard Thiocyanate-Dependent Induction of Endothelial Cell Adhesion Molecule Expression by Phagocyte Peroxidases: A Novel HOSCN-Specific Oxidant Mechanism to Amplify Inflammation J. Immunol., December 15, 2006; 177(12): 8714 - 8722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Michaud, J. Naud, J. Chouinard, F. Desy, F. A. Leblond, K. Desbiens, A. Bonnardeaux, and V. Pichette Role of Parathyroid Hormone in the Downregulation of Liver Cytochrome P450 in Chronic Renal Failure J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3041 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Smith, K. N. Maloney, R. G. Pothen, J. Clardy, and D. E. Clapham Bisandrographolide from Andrographis paniculata Activates TRPV4 Channels J. Biol. Chem., October 6, 2006; 281(40): 29897 - 29904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Iruretagoyena, S. E. Sepulveda, J. P. Lezana, M. Hermoso, M. Bronfman, M. A. Gutierrez, S. H. Jacobelli, and A. M. Kalergis Inhibition of Nuclear Factor-{kappa}B Enhances the Capacity of Immature Dendritic Cells to Induce Antigen-Specific Tolerance in Experimental Autoimmune Encephalomyelitis J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 59 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Wang, S. A. Mahmud, J. A. Thompson, J.-G. Geng, N. S. Key, and A. Slungaard The principal eosinophil peroxidase product, HOSCN, is a uniquely potent phagocyte oxidant inducer of endothelial cell tissue factor activity: a potential mechanism for thrombosis in eosinophilic inflammatory states Blood, January 15, 2006; 107(2): 558 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhou, S.-X. Zheng, W. Tang, P.-L. He, X.-Y. Li, Y.-F. Yang, Y.-C. Li, J.-G. Geng, and J.-P. Zuo Inhibition of Inducible Nitric-Oxide Synthase Expression by (5R)-5-Hydroxytriptolide in Interferon-{gamma}- and Bacterial Lipopolysaccharide-Stimulated Macrophages J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 121 - 128. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
