HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Singer, M. Articles by Sansonetti, P. J. Search for Related Content PubMed PubMed Citation Articles by Singer, M. Articles by Sansonetti, P. J.

IL-8 Is a Key Chemokine Regulating Neutrophil Recruitment in a New Mouse Model of Shigella-Induced Colitis

Unité de Pathogénie Microbienne Moléculaire, Institut National de la Santé et de la Recherche Médicale U389, Institut Pasteur, Paris, France

The lack of a mouse model of acute rectocolitis mimicking human bacillary dysentery in the presence of invasive Shigella is a major handicap to study the pathogenesis of the disease and to develop a Shigella vaccine. The inability of the mouse intestinal mucosa to elicit an inflammatory infiltrate composed primarily of polymorphonuclear leukocytes (PMN) may be due to a defect in epithelial invasion, in the sensing of invading bacteria, or in the effector mechanisms that recruit the PMN infiltrate. We demonstrate that the BALB/cJ mouse colonic epithelium not only can be invaded by Shigella, but also elicits an inflammatory infiltrate that, however, lacks PMN. This observation points to a major defect of mice in effector mechanisms, particularly the lack of expression of the CXC chemokine, IL-8. Indeed, this work demonstrates that the delivery of recombinant human IL-8, together with Shigella infection of the colonic epithelial surface, causes an acute colitis characterized by a strong PMN infiltrate that, by all criteria, including transcription profiles of key mediators of the innate/inflammatory response and histopathological lesions, mimics bacillary dysentery. This is a major step forward in the development of a murine model of bacillary dysentery.

This article has been cited by other articles:

				K. L. Mumy, J. D. Bien, M. A. Pazos, K. Gronert, B. P. Hurley, and B. A. McCormick Distinct Isoforms of Phospholipase A2 Mediate the Ability of Salmonella enterica Serotype Typhimurium and Shigella flexneri To Induce the Transepithelial Migration of Neutrophils Infect. Immun., August 1, 2008; 76(8): 3614 - 3627. [Abstract] [Full Text] [PDF]

				M.-I. Fernandez, B. Regnault, C. Mulet, M. Tanguy, P. Jay, P. J. Sansonetti, and T. Pedron Maturation of Paneth Cells Induces the Refractory State of Newborn Mice to Shigella Infection J. Immunol., April 1, 2008; 180(7): 4924 - 4930. [Abstract] [Full Text] [PDF]

				M. Chamekh, A. Phalipon, R. Quertainmont, I. Salmon, P. Sansonetti, and A. Allaoui Delivery of Biologically Active Anti-Inflammatory Cytokines IL-10 and IL-1ra In Vivo by the Shigella Type III Secretion Apparatus J. Immunol., March 15, 2008; 180(6): 4292 - 4298. [Abstract] [Full Text] [PDF]

				K. L. Mumy, X. Chen, C. P. Kelly, and B. A. McCormick Saccharomyces boulardii interferes with Shigella pathogenesis by postinvasion signaling events Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G599 - G609. [Abstract] [Full Text] [PDF]

				G. N. Schroeder and H. Hilbi Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion Clin. Microbiol. Rev., January 1, 2008; 21(1): 134 - 156. [Abstract] [Full Text] [PDF]

				T. Shimizu, Y. Kida, and K. Kuwano Mycoplasma pneumoniae-Derived Lipopeptides Induce Acute Inflammatory Responses in the Lungs of Mice Infect. Immun., January 1, 2008; 76(1): 270 - 277. [Abstract] [Full Text] [PDF]

				R. Simon, D. M. Heithoff, M. J. Mahan, and C. E. Samuel Comparison of Tissue-Selective Proinflammatory Gene Induction in Mice Infected with Wild-Type, DNA Adenine Methylase-Deficient, and Flagellin-Deficient Salmonella enterica Infect. Immun., December 1, 2007; 75(12): 5627 - 5639. [Abstract] [Full Text] [PDF]

				S. M. Williams, Y.-T. Chen, T. M. Andermann, J. E. Carter, D. J. McGee, and K. M. Ottemann Helicobacter pylori Chemotaxis Modulates Inflammation and Bacterium-Gastric Epithelium Interactions in Infected Mice Infect. Immun., August 1, 2007; 75(8): 3747 - 3757. [Abstract] [Full Text] [PDF]

				D.-H. Shim, T. Suzuki, S.-Y. Chang, S.-M. Park, P. J. Sansonetti, C. Sasakawa, and M.-N. Kweon New Animal Model of Shigellosis in the Guinea Pig: Its Usefulness for Protective Efficacy Studies J. Immunol., February 15, 2007; 178(4): 2476 - 2482. [Abstract] [Full Text] [PDF]

				K. R. Bracke, A. I. D'hulst, T. Maes, K. B. Moerloose, I. K. Demedts, S. Lebecque, G. F. Joos, and G. G. Brusselle Cigarette Smoke-Induced Pulmonary Inflammation and Emphysema Are Attenuated in CCR6-Deficient Mice J. Immunol., October 1, 2006; 177(7): 4350 - 4359. [Abstract] [Full Text] [PDF]

				E. A. Burton, A. M. Pendergast, and A. Aballay The Caenorhabditis elegans ABL-1 Tyrosine Kinase Is Required for Shigella flexneri Pathogenesis. Appl. Envir. Microbiol., July 1, 2006; 72(7): 5043 - 5051. [Abstract] [Full Text] [PDF]

				K. P. Allen, M. M. Randolph, and J. M. Fleckenstein Importance of Heat-Labile Enterotoxin in Colonization of the Adult Mouse Small Intestine by Human Enterotoxigenic Escherichia coli Strains Infect. Immun., February 1, 2006; 74(2): 869 - 875. [Abstract] [Full Text] [PDF]