| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departaments of
*
Bioquímica e Imunologia e
Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Pampulha, Belo Horizonte, Brazil
The restoration of blood flow, i.e., reperfusion, is the treatment of choice to save viable tissue following acute ischemia of a vascular territory. Nevertheless, reperfusion can be accompanied by significant inflammatory events that limit the beneficial effects of blood flow restoration. To evaluate the potential role of the intestinal microbiota in facilitating the development of tissue injury and systemic inflammation, germ-free and conventional mice were compared in their ability to respond to ischemia and reperfusion injury. In conventional mice, there was marked local (intestine) and remote (lung) edema formation, neutrophil influx, hemorrhage, and production of TNF-
, KC, MIP-2, and MCP-1. Moreover, there was an increase in the concentration of serum TNF- and 100% lethality. In germ-free mice, there was no local, remote, or systemic inflammatory response or lethality after intestinal ischemia and reperfusion and, in contrast to conventional mice, germ-free animals produced greater amounts of IL-10. Similar results were obtained after administration of LPS, i.e., little production of TNF- or lethality and production of IL-10 after LPS in germ-free mice. Blockade of IL-10 with Abs induced marked inflammation and lethality in germ-free mice after ischemia and reperfusion or LPS administration, demonstrating that the ability of these mice to produce IL-10 was largely responsible for their "no inflammation" phenotype. This was consistent with the prevention of reperfusion-associated injury by the exogenous administration of IL-10 to conventional mice. Thus, the lack of intestinal microbiota is accompanied by a state of active IL-10-mediated inflammatory hyporesponsiveness.
This article has been cited by other articles:
|
|
 |
|
  E. S. Klaassens, R. J. Boesten, M. Haarman, J. Knol, F. H. Schuren, E. E. Vaughan, and W. M. de Vos Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants Appl. Envir. Microbiol., May 1, 2009; 75(9): 2668 - 2676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Souza, F. A. Amaral, C. T. Fagundes, F. M. Coelho, R. M.E. Arantes, L. P. Sousa, M. M. Matzuk, C. Garlanda, A. Mantovani, A. A. Dias, et al. The Long Pentraxin PTX3 Is Crucial for Tissue Inflammation after Intestinal Ischemia and Reperfusion in Mice Am. J. Pathol., April 1, 2009; 174(4): 1309 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Amaral, D. Sachs, V. V. Costa, C. T. Fagundes, D. Cisalpino, T. M. Cunha, S. H. Ferreira, F. Q. Cunha, T. A. Silva, J. R. Nicoli, et al. Commensal microbiota is fundamental for the development of inflammatory pain PNAS, February 12, 2008; 105(6): 2193 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Souza, C. T. Fagundes, F. A. Amaral, D. Cisalpino, L. P. Sousa, A. T. Vieira, V. Pinho, J. R. Nicoli, L. Q. Vieira, I. M. Fierro, et al. The Required Role of Endogenously Produced Lipoxin A4 and Annexin-1 for the Production of IL-10 and Inflammatory Hyporesponsiveness in Mice J. Immunol., December 15, 2007; 179(12): 8533 - 8543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Barnes, A. Vidrich, M.-L. Wang, G. D. Wu, F. Cominelli, J. Rivera-Nieves, G. Bamias, and S. M. Cohn Resistin-Like Molecule beta (RELMbeta/FIZZ2) Is Highly Expressed in the Ileum of SAMP1/YitFc Mice and Is Associated with Initiation of Ileitis J. Immunol., November 15, 2007; 179(10): 7012 - 7020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Fagundes, F. A. Amaral, A. L. S. Souza, A. T. Vieira, D. Xu, F. Y. Liew, D. G. Souza, and M. M. Teixeira ST2, an IL-1R family member, attenuates inflammation and lethality after intestinal ischemia and reperfusion J. Leukoc. Biol., February 1, 2007; 81(2): 492 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lievin-Le Moal and A. L. Servin The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota Clin. Microbiol. Rev., April 1, 2006; 19(2): 315 - 337. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
