The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sturm, A.
Right arrow Articles by Gabius, H.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sturm, A.
Right arrow Articles by Gabius, H.-J.
The Journal of Immunology, 2004, 173: 3825-3837.
Copyright © 2004 by The American Association of Immunologists

Human Galectin-2: Novel Inducer of T Cell Apoptosis with Distinct Profile of Caspase Activation1

Andreas Sturm2,*, Martin Lensch{dagger}, Sabine André{dagger}, Herbert Kaltner{dagger}, Bertram Wiedenmann*, Stefan Rosewicz*, Axel U. Dignass* and Hans-Joachim Gabius{dagger}

* Medizinische Klinik mit Sektion Hepatologie und Gastroenterologie, Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany; and {dagger} Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität München, Munich, Germany

Galectin-2 is structurally closely related to galectin-1, but has a distinct expression profile primarily confined to the gastrointestinal tract. Prominent differences in the proximal promoter regions between galectins-2 and -1 concern Sp1-, hepatocyte NF-3, and T cell-specific factor-1 binding sites. Of note, these sequence elements are positioned equally in the respective regions for human and rat galectins-2. Labeled galectin-2 binds to T cells in a {beta}-galactoside-specific manner. In contrast to galectin-1, the glycoproteins CD3 and CD7 are not ligands, while the shared affinity to {beta}1 integrin (or a closely associated glycoprotein) accounts for a substantial extent of cell surface binding. The carbohydrate-dependent binding of galectin-2 induces apoptosis in activated T cells. Fluorogenic substrate and inhibitor assays reveal involvement of caspases-3 and -9, in accordance with cleavage of the DNA fragmentation factor. Enhanced cytochrome c release, disruption of the mitochondrial membrane potential, and an increase of the Bax/Bcl-2 ratio by opposite regulation of expression of both proteins add to the evidence that the intrinsic apoptotic pathway is triggered. Cell cycle distribution and expression of regulatory proteins remained unaffected. Notably, galectins-1 and -7 reduce cyclin B1 expression, defining functional differences between the structurally closely related galectins. Cytokine secretion of activated T cells was significantly shifted to the Th2 profile. Our study thus classifies galectin-2 as proapoptotic effector for activated T cells, raising a therapeutic perspective. Of importance for understanding the complex galectin network, it teaches the lesson that selection of cell surface ligands, route of signaling, and effects on regulators of cell cycle progression are markedly different between structurally closely related galectins.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
C. Saravanan, F.-T. Liu, I. K. Gipson, and N. Panjwani
Galectin-3 promotes lamellipodia formation in epithelial cells by interacting with complex N-glycans on {alpha}3{beta}1 integrin
J. Cell Sci., October 15, 2009; 122(20): 3684 - 3693.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. V. Tribulatti, V. Cattaneo, U. Hellman, J. Mucci, and O. Campetella
Galectin-8 provides costimulatory and proliferative signals to T lymphocytes
J. Leukoc. Biol., August 1, 2009; 86(2): 371 - 380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Norambuena, C. Metz, L. Vicuna, A. Silva, E. Pardo, C. Oyanadel, L. Massardo, A. Gonzalez, and A. Soza
Galectin-8 Induces Apoptosis in Jurkat T Cells by Phosphatidic Acid-mediated ERK1/2 Activation Supported by Protein Kinase A Down-regulation
J. Biol. Chem., May 8, 2009; 284(19): 12670 - 12679.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Loser, A. Sturm, M. Voskort, V. Kupas, S. Balkow, M. Auriemma, C. Sternemann, A. U. Dignass, T. A. Luger, and S. Beissert
Galectin-2 Suppresses Contact Allergy by Inducing Apoptosis in Activated CD8+ T Cells
J. Immunol., May 1, 2009; 182(9): 5419 - 5429.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. J. Garcia-Pineres, A. Hildesheim, L. Dodd, T. J. Kemp, J. Yang, B. Fullmer, C. Harro, D. R. Lowy, R. A. Lempicki, and L. A. Pinto
Gene Expression Patterns Induced by HPV-16 L1 Virus-Like Particles in Leukocytes from Vaccine Recipients
J. Immunol., February 1, 2009; 182(3): 1706 - 1729.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
W. Peng, H. Y. Wang, Y. Miyahara, G. Peng, and R.-F. Wang
Tumor-Associated Galectin-3 Modulates the Function of Tumor-Reactive T Cells
Cancer Res., September 1, 2008; 68(17): 7228 - 7236.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
C. Guzy, D. Paclik, A. Schirbel, U. Sonnenborn, B. Wiedenmann, and A. Sturm
The probiotic Escherichia coli strain Nissle 1917 induces {gamma}{delta} T cell apoptosis via caspase- and FasL-dependent pathways
Int. Immunol., July 1, 2008; 20(7): 829 - 840.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Bi, L. A. Earl, L. Jacobs, and L. G. Baum
Structural Features of Galectin-9 and Galectin-1 That Determine Distinct T Cell Death Pathways
J. Biol. Chem., May 2, 2008; 283(18): 12248 - 12258.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Stowell, C. M. Arthur, P. Mehta, K. A. Slanina, O. Blixt, H. Leffler, D. F. Smith, and R. D. Cummings
Galectin-1, -2, and -3 Exhibit Differential Recognition of Sialylated Glycans and Blood Group Antigens
J. Biol. Chem., April 11, 2008; 283(15): 10109 - 10123.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Pilette, B. Colinet, R. Kiss, S. Andre, H. Kaltner, H-J. Gabius, M. Delos, J-P. Vaerman, M. Decramer, and Y. Sibille
Increased galectin-3 expression and intra-epithelial neutrophils in small airways in severe COPD
Eur. Respir. J., May 1, 2007; 29(5): 914 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Demers, K. Biron-Pain, J. Hebert, A. Lamarre, T. Magnaldo, and Y. St-Pierre
Galectin-7 in Lymphoma: Elevated Expression in Human Lymphoid Malignancies and Decreased Lymphoma Dissemination by Antisense Strategies in Experimental Model
Cancer Res., March 15, 2007; 67(6): 2824 - 2829.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. J. Van Dyken, R. S. Green, and J. D. Marth
Structural and Mechanistic Features of Protein O Glycosylation Linked to CD8+ T-Cell Apoptosis
Mol. Cell. Biol., February 1, 2007; 27(3): 1096 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. M. Wu, T. Singh, J.-H. Liu, M. Krzeminski, R. Russwurm, H.-C. Siebert, A. M.J.J. Bonvin, S. Andre, and H.-J. Gabius
Activity-structure correlations in divergent lectin evolution: fine specificity of chicken galectin CG-14 and computational analysis of flexible ligand docking for CG-14 and the closely related CG-16
Glycobiology, February 1, 2007; 17(2): 165 - 184.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
L.-H. Lu, R. Nakagawa, Y. Kashio, A. Ito, H. Shoji, N. Nishi, M. Hirashima, A. Yamauchi, and T. Nakamura
Characterization of Galectin-9-Induced Death of Jurkat T Cells
J. Biochem., February 1, 2007; 141(2): 157 - 172.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Barrionuevo, M. Beigier-Bompadre, J. M. Ilarregui, M. A. Toscano, G. A. Bianco, M. A. Isturiz, and G. A. Rabinovich
A Novel Function for Galectin-1 at the Crossroad of Innate and Adaptive Immunity: Galectin-1 Regulates Monocyte/Macrophage Physiology through a Nonapoptotic ERK-Dependent Pathway
J. Immunol., January 1, 2007; 178(1): 436 - 445.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. R. Stowell, S. Karmakar, C. J. Stowell, M. Dias-Baruffi, R. P. McEver, and R. D. Cummings
Human galectin-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not in activated T cells
Blood, January 1, 2007; 109(1): 219 - 227.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Ruby, C. Whittaker, D. R. Withers, M. K. Chelbi-Alix, V. Morin, A. Oudin, J. R. Young, and R. Zoorob
Transcriptional profiling reveals a possible role for the timing of the inflammatory response in determining susceptibility to a viral infection.
J. Virol., September 1, 2006; 80(18): 9207 - 9216.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. M. Wu, T. Singh, J. H. Wu, M. Lensch, S. Andre, and H.-J. Gabius
Interaction profile of galectin-5 with free saccharides and mammalian glycoproteins: probing its fine specificity and the effect of naturally clustered ligand presentation
Glycobiology, June 1, 2006; 16(6): 524 - 537.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. Toscano, A. G. Commodaro, J. M. Ilarregui, G. A. Bianco, A. Liberman, H. M. Serra, J. Hirabayashi, L. V. Rizzo, and G. A. Rabinovich
Galectin-1 Suppresses Autoimmune Retinal Disease by Promoting Concomitant Th2- and T Regulatory-Mediated Anti-Inflammatory Responses
J. Immunol., May 15, 2006; 176(10): 6323 - 6332.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
E. Ish-Shalom, A. Gargir, S. Andre, Z. Borovsky, Z. Ochanuna, H.-J. Gabius, M. L. Tykocinski, and J. Rachmilewitz
{alpha}2,6-Sialylation promotes binding of placental protein 14 via its Ca2+-dependent lectin activity: insights into differential effects on CD45RO and CD45RA T cells
Glycobiology, March 1, 2006; 16(3): 173 - 183.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. N. Stillman, D. K. Hsu, M. Pang, C. F. Brewer, P. Johnson, F.-T. Liu, and L. G. Baum
Galectin-3 and Galectin-1 Bind Distinct Cell Surface Glycoprotein Receptors to Induce T Cell Death
J. Immunol., January 15, 2006; 176(2): 778 - 789.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Fischer, H. Sanchez-Ruderisch, M. Welzel, B. Wiedenmann, T. Sakai, S. Andre, H.-J. Gabius, L. Khachigian, K. M. Detjen, and S. Rosewicz
Galectin-1 Interacts with the {alpha}5{beta}1 Fibronectin Receptor to Restrict Carcinoma Cell Growth via Induction of p21 and p27
J. Biol. Chem., November 4, 2005; 280(44): 37266 - 37277.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
J M Ilarregui, G A Bianco, M A Toscano, and G A Rabinovich
The coming of age of galectins as immunomodulatory agents: impact of these carbohydrate binding proteins in T cell physiology and chronic inflammatory disorders
Ann Rheum Dis, November 1, 2005; 64(suppl_4): iv96 - iv103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Matarrese, A. Tinari, E. Mormone, G. A. Bianco, M. A. Toscano, B. Ascione, G. A. Rabinovich, and W. Malorni
Galectin-1 Sensitizes Resting Human T Lymphocytes to Fas (CD95)-mediated Cell Death via Mitochondrial Hyperpolarization, Budding, and Fission
J. Biol. Chem., February 25, 2005; 280(8): 6969 - 6985.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.