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The Journal of Immunology, 2004, 173: 3044-3050.
Copyright © 2004 by The American Association of Immunologists

Opsonization with C1q and Mannose-Binding Lectin Targets Apoptotic Cells to Dendritic Cells1

Alma J. Nauta, Giuseppe Castellano, Wei Xu, Andrea M. Woltman, Maria C. Borrias, Mohamed R. Daha, Cees van Kooten and Anja Roos2

Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands

Deficiencies of early components of the classical complement pathway, particularly C1q, are strongly associated with susceptibility to systemic lupus erythematosus. Recent data link this predisposal to autoimmunity to an inappropriate clearance of apoptotic cells, which could lead to a loss of self-tolerance. In the present study, we demonstrate that opsonization of apoptotic cells with C1q and mannose-binding lectin allows and facilitates their uptake not only by macrophages but also by human immature dendritic cells (DCs). Both C1q and mannose-binding lectin enhance the uptake of apoptotic cells by DCs in a dose-dependent way. The uptake of C1q-opsonized apoptotic cells, but not nonopsonized apoptotic cells, by DCs stimulated the production of IL-6, IL-10, and TNF-{alpha}, without an effect on IL-12p70. We conclude that these recognition molecules of the complement system do not sequester apoptotic cells from DCs, but rather promote their uptake by immature DCs. Therefore, we propose that early complement components support safe clearance of cellular debris by facilitating phagocytosis and possibly by immunomodulatory mechanisms, thus preventing autoimmunity.




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