| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Infectious, Parasitic and Immune-Mediated Diseases and
Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy;
Division of Rheumatology, Azienda Ospedaliera San Camillo-Forlanini, Rome, Italy;
Division of Orthopedics, University of Tor Vergata, Rome, Italy; and
¶ Division of Rheumatology, University of Rome "La Sapienza," Rome, Italy
Dendritic cells (DCs) are thought to play a key role in driving the immunopathogenic response underlying chronic inflammatory arthritis. In this study, we have examined the presence and phenotype of plasmacytoid DCs (pDCs) in the synovial fluids (SF) of patients with rheumatoid arthritis (RA), psoriatic arthritis (PA), and osteoarthritis (OA) and determined the chemotactic properties of SF from these patients toward pDCs. Flow cytometry analysis showed that the percentage of pDCs, identified as a population of Lin–CD123++ cells, is 4- to 5-fold higher in RA SF and PA SF than in OA SF. The morphological and immunophenotypic characterization of pDCs isolated from PA and RA SF indicates that they are in an immature state, most likely due to inhibitory factors present in RA SF, but are still able to undergo maturation when exposed ex vivo to viral agent or unmethylated DNA. CD123+ and BDCA2+ pDCs were detected by immunohistochemistry in RA synovial tissue in which expression of the IFN-
-inducible protein MxA was also found, suggesting production of type I IFN by maturing pDCs. We also show that CXCR3 and CXCR4 are expressed by both blood-derived pDCs and pDCs isolated from RA and PA SF and that CXCL-10, CXCL-11, and CXCL-12 present in RA and PA SF stimulate chemotaxis of blood-derived pDCs. Altogether, these findings suggest that chemokine-driven recruitment of pDCs from the blood to the inflamed synovium could be important in the regulation of the immune response in chronic inflammatory arthritis.
This article has been cited by other articles:
|
|
 |
|
  S. L. Jongbloed, R. A. Benson, M. B. Nickdel, P. Garside, I. B. McInnes, and J. M. Brewer Plasmacytoid Dendritic Cells Regulate Breach of Self-Tolerance in Autoimmune Arthritis J. Immunol., January 15, 2009; 182(2): 963 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Ju, M. Zenke, D. N. J. Hart, and G. J. Clark CD300a/c regulate type I interferon and TNF-{alpha} secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands Blood, August 15, 2008; 112(4): 1184 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Smolewska, J Stanczyk, H Brozik, M Biernacka-Zielinska, B Cebula, T Robak, and P Smolewski Distribution and clinical significance of blood dendritic cells in children with juvenile idiopathic arthritis Ann Rheum Dis, June 1, 2008; 67(6): 762 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Serafini, B. Rosicarelli, D. Franciotta, R. Magliozzi, R. Reynolds, P. Cinque, L. Andreoni, P. Trivedi, M. Salvetti, A. Faggioni, et al. Dysregulated Epstein-Barr virus infection in the multiple sclerosis brain J. Exp. Med., November 26, 2007; 204(12): 2899 - 2912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J G Walker, M J Ahern, M Coleman, H Weedon, V Papangelis, D Beroukas, P J Roberts-Thomson, and M D Smith Characterisation of a dendritic cell subset in synovial tissue which strongly expresses Jak/STAT transcription factors from patients with rheumatoid arthritis Ann Rheum Dis, August 1, 2007; 66(8): 992 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gattorno, L. Chicha, A. Gregorio, F. Ferlito, F. Rossi, D. Jarrossay, A. Lanzavecchia, A. Martini, and M. G. Manz Distinct expression pattern of IFN-{alpha} and TNF-{alpha} in juvenile idiopathic arthritis synovial tissue Rheumatology, April 1, 2007; 46(4): 657 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. F. Porter, A. Ambrus, and R. W. Storts Immunohistochemical evaluation of mx protein expression in canine encephalitides. Vet. Pathol., November 1, 2006; 43(6): 981 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-E. Gottenberg, N. Cagnard, C. Lucchesi, F. Letourneur, S. Mistou, T. Lazure, S. Jacques, N. Ba, M. Ittah, C. Lepajolec, et al. Activation of IFN pathways and plasmacytoid dendritic cell recruitment in target organs of primary Sjogren's syndrome PNAS, February 21, 2006; 103(8): 2770 - 2775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Salazar, C. D. Pope, M. W. Moore, J. Pope, T. G. Kiely, and J. D. Radolf Lipoprotein-Dependent and -Independent Immune Responses to Spirochetal Infection Clin. Vaccine Immunol., August 1, 2005; 12(8): 949 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. O. Nestle, C. Conrad, A. Tun-Kyi, B. Homey, M. Gombert, O. Boyman, G. Burg, Y.-J. Liu, and M. Gilliet Plasmacytoid predendritic cells initiate psoriasis through interferon-{alpha} production J. Exp. Med., July 5, 2005; 202(1): 135 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Zabel, A. M. Silverio, and E. C. Butcher Chemokine-Like Receptor 1 Expression and Chemerin-Directed Chemotaxis Distinguish Plasmacytoid from Myeloid Dendritic Cells in Human Blood J. Immunol., January 1, 2005; 174(1): 244 - 251. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
