| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Pediatrics and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555;
Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65212; and
National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, IA 50010
Granulysin and NK-lysin are antimicrobial proteins found in the granules of human and swine cytotoxic lymphocytes. A murine counterpart to granulysin has not been identified to date, indicating the importance of additional models to fully characterize the role of granulysin-like molecules in the immune response to infectious disease. Two partial nucleotide sequences corresponding to the complete functional domain of granulysin and NK-lysin were amplified from bovine PBMC mRNA. Following stimulation with phorbol ester and calcium ionophore, expression of the bovine gene was detected in CD3+ T cells, CD4+ T cells, CD8+ T cells, WC1+ 
T cells, and PBMC depleted of CD3+ T cells, but was absent in CD21+ cells and CD14+ cells. Intracellular flow cytometry and immunoblotting confirmed the presence of protein corresponding to the bovine granulysin homologue in activated T lymphocytes and PBMC. Synthetic human, bovine, and swine peptides corresponding to the C terminus of helix 2 through helix 3 region of granulysin displayed potent antimicrobial activity against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Mycobacterium bovis bacillus Calmette-Guérin. Human and bovine peptides corresponding to helix 2 displayed antimycobacterial activity against M. bovis bacillus Calmette-Guérin. Expression of the bovine gene was detected in laser microscopy-dissected lymph node lesions from an M. bovis-infected animal. The identification of a biologically active bovine homologue to granulysin demonstrates the potential of the bovine model in characterizing the role of granulysin in the immune response to a variety of infectious agents.
This article has been cited by other articles:
|
|
 |
|
  L. P. Huang, S.-C. Lyu, C. Clayberger, and A. M. Krensky Granulysin-Mediated Tumor Rejection in Transgenic Mice J. Immunol., January 1, 2007; 178(1): 77 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Andra, T. Gutsmann, P. Garidel, and K. Brandenburg Invited review: Mechanisms of endotoxin neutralization by synthetic cationic compounds Innate Immunity, October 1, 2006; 12(5): 261 - 277. [Abstract] [PDF]
|
|
|
|
 |
|
 J. J. Endsley, M. A. Endsley, and D. M. Estes Bovine natural killer cells acquire cytotoxic/effector activity following activation with IL-12/15 and reduce Mycobacterium bovis BCG in infected macrophages J. Leukoc. Biol., January 1, 2006; 79(1): 71 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. A. Linde, S. Grundstrom, E. Nordling, E. Refai, P. J. Brennan, and M. Andersson Conserved Structure and Function in the Granulysin and NK-Lysin Peptide Family Infect. Immun., October 1, 2005; 73(10): 6332 - 6339. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
