HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thompson, A. G. Articles by Thomas, R. Search for Related Content PubMed PubMed Citation Articles by Thompson, A. G. Articles by Thomas, R.

T Cells Signaled by NF-B^– Dendritic Cells Are Sensitized Not Anergic to Subsequent Activation¹

Centre for Immunology and Cancer Research, Princess Alexandra Hospital, University of Queensland, Brisbane, Australia

Paradoxically, while peripheral self-tolerance exists for constitutively presented somatic self Ag, self-peptide recognized in the context of MHC class II has been shown to sensitize T cells for subsequent activation. We have shown that MHC class II⁺CD86⁺CD40^– DC, which can be generated from bone marrow in the presence of an NF-B inhibitor, and which constitutively populate peripheral tissues and lymphoid organs in naive animals, can induce Ag-specific tolerance. In this study, we show that CD40^– human monocyte-derived dendritic cells (DC), generated in the presence of an NF-B inhibitor, signal phosphorylation of TCR, but little proliferation or IFN- in vitro. Proliferation is arrested in the G₁/G₀ phase of the cell cycle. Surprisingly, responding T cells are neither anergic nor regulatory, but are sensitized for subsequent IFN- production. The data indicate that signaling through NF-B determines the capacity of DC to stimulate T cell proliferation. Functionally, NF-B^–CD40^–class II⁺ DC may either tolerize or sensitize T cells. Thus, while CD40^– DC appear to "prime" or prepare T cells, the data imply that signals derived from other cells drive the generation either of Ag-specific regulatory or effector cells in vivo.

This article has been cited by other articles:

				A. Aldinucci, G. Gerlini, S. Fossati, G. Cipriani, C. Ballerini, T. Biagioli, N. Pimpinelli, L. Borgognoni, L. Massacesi, F. Moroni, et al. A Key Role for Poly(ADP-Ribose) Polymerase-1 Activity during Human Dendritic Cell Maturation J. Immunol., July 1, 2007; 179(1): 305 - 312. [Abstract] [Full Text] [PDF]