The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hafsi, N.
Right arrow Articles by Prinz, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hafsi, N.
Right arrow Articles by Prinz, C.
The Journal of Immunology, 2004, 173: 1249-1257.
Copyright © 2004 by The American Association of Immunologists

Human Dendritic Cells Respond to Helicobacter pylori, Promoting NK Cell and Th1-Effector Responses In Vitro1

Nadia Hafsi, Petra Voland, Susanne Schwendy, Roland Rad, Wolfgang Reindl, Markus Gerhard and Christian Prinz2

Department of Medicine II, Technical University, Munich, Germany

Helicobacter pylori infection leads to chronic gastric inflammation. The current study determined the response of human APCs, NK cells, and T cells toward the bacteria in vitro. Human monocyte-derived dendritic cells (DC) were incubated with bacteria for 48 h. Intact H. pylori at a multitude of infection 5 stimulated the expression of MHC class II (4- to 7-fold), CD80, and CD86 B7 molecules (10- to 12-fold) and the CD83 costimulatory molecule (>30-fold) as well as IL-12 secretion (>50-fold) in DCs, and thereby, strongly induced their maturation and activation. CD56+/CD4– NK cells, as well as CD4+/CD45RA+ naive T cells, were isolated and incubated with DCs pulsed with intact bacteria or different cellular fractions. Coculture of H. pylori-pulsed DCs with NK cells strongly potentiated the secretion of TNF-{alpha} and IFN-{gamma}. Coculture of naive T cells with H. pylori-pulsed DCs significantly enhanced TNF-{alpha}, IFN-{gamma}, and IL-2 secretion as well as T-bet mRNA levels, while GATA-3 mRNA was lowered. However, the effect appeared attenuated compared with coculture with Escherichia coli. A greater stimulation was seen with naive T cells and DCs pulsed with H. pylori membrane preparations. Intact H. pylori potently induced the maturation and activation of human monocyte-derived DC and thereby promote NK and Th1 effector responses. The strong activation of NK cells may be important for the innate immune response. Th1-polarized T cells were induced especially by incubation with membrane preparations of H. pylori, suggesting that membrane proteins may account for the specific adaptive immune response.




This article has been cited by other articles:


Home page
 Int ImmunolHome page
L. N. Fink, L. H. Zeuthen, H. R. Christensen, B. Morandi, H. Frokiaer, and G. Ferlazzo
Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses
Int. Immunol., December 1, 2007; 19(12): 1319 - 1327.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Tomimori, E. Uema, H. Teruya, C. Ishikawa, T. Okudaira, M. Senba, K. Yamamoto, T. Matsuyama, F. Kinjo, J. Fujita, et al.
Helicobacter pylori Induces CCL20 Expression
Infect. Immun., November 1, 2007; 75(11): 5223 - 5232.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Nagai, H. Mimuro, T. Yamada, Y. Baba, K. Moro, T. Nochi, H. Kiyono, T. Suzuki, C. Sasakawa, and S. Koyasu
Role of Peyer's patches in the induction of Helicobacter pylori-induced gastritis
PNAS, May 22, 2007; 104(21): 8971 - 8976.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. Mandang, U. Manuelpillai, and E. M Wallace
Oxidative stress increases placental and endothelial cell activin A secretion
J. Endocrinol., March 1, 2007; 192(3): 485 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Mitchell, C. Germain, P. L. Fiori, W. Khamri, G. R. Foster, S. Ghosh, R. I. Lechler, K. B. Bamford, and G. Lombardi
Chronic Exposure to Helicobacter pylori Impairs Dendritic Cell Function and Inhibits Th1 Development
Infect. Immun., February 1, 2007; 75(2): 810 - 819.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
L. Hou, E.M. El-Omar, J. Chen, P. Grillo, C.S. Rabkin, A. Baccarelli, M. Yeager, S.J. Chanock, W. Zatonski, L.H. Sobin, et al.
Polymorphisms in Th1-type cell-mediated response genes and risk of gastric cancer
Carcinogenesis, January 1, 2007; 28(1): 118 - 123.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
H. M. S. Algood and T. L. Cover
Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses
Clin. Microbiol. Rev., October 1, 2006; 19(4): 597 - 613.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Alnaeeli, J. M. Penninger, and Y.-T. A. Teng
Immune Interactions with CD4+ T Cells Promote the Development of Functional Osteoclasts from Murine CD11c+ Dendritic Cells.
J. Immunol., September 1, 2006; 177(5): 3314 - 3326.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. L. Drakes, S. J. Czinn, and T. G. Blanchard
Regulation of Murine Dendritic Cell Immune Responses by Helicobacter felis Antigen.
Infect. Immun., August 1, 2006; 74(8): 4624 - 4633.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers
Pathogenesis of Helicobacter pylori Infection
Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Y. Kao, S. Rathinavelu, K. A. Eaton, L. Bai, Y. Zavros, M. Takami, A. Pierzchala, and J. L. Merchant
Helicobacter pylori-secreted factors inhibit dendritic cell IL-12 secretion: a mechanism of ineffective host defense
Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G73 - G81.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. Deml, M. Aigner, J. Decker, A. Eckhardt, C. Schutz, P. R. E. Mittl, S. Barabas, S. Denk, G. Knoll, N. Lehn, et al.
Characterization of the Helicobacter pylori Cysteine-Rich Protein A as a T-Helper Cell Type 1 Polarizing Agent
Infect. Immun., August 1, 2005; 73(8): 4732 - 4742.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
D. Pinto-Santini and N. R. Salama
The Biology of Helicobacter pylori Infection, a Major Risk Factor for Gastric Adenocarcinoma
Cancer Epidemiol. Biomarkers Prev., August 1, 2005; 14(8): 1853 - 1858.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Kranzer, L. Sollner, M. Aigner, N. Lehn, L. Deml, M. Rehli, and W. Schneider-Brachert
Impact of Helicobacter pylori Virulence Factors and Compounds on Activation and Maturation of Human Dendritic Cells
Infect. Immun., July 1, 2005; 73(7): 4180 - 4189.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. H. Yun, A. Lundgren, J. Azem, A. Sjoling, J. Holmgren, A.-M. Svennerholm, and B. S. Lundin
Natural Killer Cells and Helicobacter pylori Infection: Bacterial Antigens and Interleukin-12 Act Synergistically To Induce Gamma Interferon Production
Infect. Immun., March 1, 2005; 73(3): 1482 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. T. Kamath, C. E. Sheasby, and D. F. Tough
Dendritic Cells and NK Cells Stimulate Bystander T Cell Activation in Response to TLR Agonists through Secretion of IFN-{alpha}{beta} and IFN-{gamma}
J. Immunol., January 15, 2005; 174(2): 767 - 776.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.