HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Viertlboeck, B. C. Articles by Göbel, T. W. F. Search for Related Content PubMed PubMed Citation Articles by Viertlboeck, B. C. Articles by Göbel, T. W. F.

Chicken Ig-Like Receptor B2, a Member of a Multigene Family, Is Mainly Expressed on B Lymphocytes, Recruits Both Src Homology 2 Domain Containing Protein Tyrosine Phosphatase (SHP)-1 and SHP-2, and Inhibits Proliferation¹

Birgit C. Viertlboeck^*, Richard P. M. A. Crooijmans, Martien A. M. Groenen and Thomas W. F. Göbel^2,*

^* Institute for Animal Physiology, University of Munich, Munich, Germany; and Animal Breeding and Genetics Group, Wageningen University, Wageningen, The Netherlands

Ig-like inhibitory receptors have been the focus of intensive research particularly in mouse and human. We report the cloning and characterization of three novel inhibitory chicken Ig-like receptors (CHIR) that display a two Ig-domain extracellular structure, a transmembrane region lacking charged residues and a cytoplasmic domain containing two ITIM. The localization of all receptors to a small genomic region and the hybridization pattern indicated that they belong to a multigene family. The genomic structure of the extracellular domain with two exons encoding the signal peptide and single exons for each Ig domain resembled that of all human leukocyte Ig-like receptors and killer cell Ig-like receptors, whereas the exons encoding the C terminus displayed a structure closely resembling killer cell Ig-like receptor genes. A mAb generated against one receptor designated CHIR-B2 reacted with all B cells and a small T cell subset, but not with monocytes, thrombocytes, or various leukocyte-derived cell lines. The mAb immunoprecipitated a 46-kDa protein from bursal cells and transfected cells. The Src homology 2 domain containing protein tyrosine phosphatase (SHP)-2 bound to CHIR-B2 even in unstimulated cells, whereas pervanadate treatment induced the tyrosine phosphorylation and recruitment of several CHIR-B2-associated proteins including SHP-1 and increased levels of SHP-2. Moreover, mAb cross-linking of CHIR-B2 reduced the proliferation of a stable transfected cell line. Together, we have identified a multigene family containing multiple CHIR including one receptor designated CHIR-B2 that is mainly expressed on B lymphocytes and inhibits cellular proliferation by recruitment of SHP-1 and SHP-2.

This article has been cited by other articles:

				B. C. Viertlboeck, S. Schweinsberg, M. A. Hanczaruk, R. Schmitt, L. Du Pasquier, F. W. Herberg, and T. W. Gobel The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor PNAS, July 10, 2007; 104(28): 11718 - 11723. [Abstract] [Full Text] [PDF]

				B. C. Viertlboeck, F. A. Habermann, R. Schmitt, M. A. M. Groenen, L. Du Pasquier, and T. W. Gobel The Chicken Leukocyte Receptor Complex: A Highly Diverse Multigene Family Encoding at Least Six Structurally Distinct Receptor Types J. Immunol., July 1, 2005; 175(1): 385 - 393. [Abstract] [Full Text] [PDF]

				N. Nikolaidis, I. Makalowska, D. Chalkia, W. Makalowski, J. Klein, and M. Nei Origin and evolution of the chicken leukocyte receptor complex PNAS, March 15, 2005; 102(11): 4057 - 4062. [Abstract] [Full Text] [PDF]