The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balabanian, K.
Right arrow Articles by Chakrabarti, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balabanian, K.
Right arrow Articles by Chakrabarti, L. A.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*CALCIUM COMPOUNDS
*CALCIUM, ELEMENTAL
The Journal of Immunology, 2004, 173: 7150-7160.
Copyright © 2004 by The American Association of Immunologists

CXCR4-Tropic HIV-1 Envelope Glycoprotein Functions as a Viral Chemokine in Unstimulated Primary CD4+ T Lymphocytes1

Karl Balabanian2,*, Julie Harriague2,*, Christine Décrion*, Bernard Lagane*, Spencer Shorte{dagger}, Françoise Baleux{ddagger}, Jean-Louis Virelizier*, Fernando Arenzana-Seisdedos* and Lisa A. Chakrabarti3,*

* Viral Immunology Unit, {dagger} Platform for Dynamic Imaging, and {ddagger} Organic Chemistry Unit, Pasteur Institute, Paris, France

Interaction of HIV-1 envelope glycoprotein gp120 with the chemokine receptor CXCR4 triggers not only viral entry but also an array of signal transduction cascades. Whether gp120 induces an incomplete or aberrant set of signals, or whether it can function as a full CXCR4 agonist, remains unclear. We report that, in unstimulated human primary CD4+ T cells, the spectrum of signaling responses induced by gp120 through CXCR4 paralleled that induced by the natural ligand stromal cell-derived factor 1/CXCL12. gp120 activated heterotrimeric G proteins and the major G protein-dependent pathways, including calcium mobilization, phosphoinositide-3 kinase, and Erk-1/2 MAPK activation. Interestingly, gp120 caused rapid actin cytoskeleton rearrangements and profuse membrane ruffling, as evidenced by dynamic confocal imaging. This coordinated set of events resulted in a bona fide chemotactic response. Inactivated HIV-1 virions that harbored conformationally intact envelope glycoproteins also caused actin polymerization and chemotaxis, while similar virions devoid of envelope glycoproteins did not. Thus gp120, in monomeric as well as oligomeric, virion-associated form, elicited a complex cellular response that mimicked the effects of a chemokine. HIV-1 has therefore the capacity to dysregulate the vast CD4+ T cell population that expresses CXCR4. In addition, HIV-1 may exploit its chemotactic properties to retain potential target cells and locally perturb their cytoskeleton, thereby facilitating viral transmission.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. R. Campbell and S. A. Spector
CCL2 Increases X4-tropic HIV-1 Entry into Resting CD4+ T Cells
J. Biol. Chem., November 7, 2008; 283(45): 30745 - 30753.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hachet-Haas, K. Balabanian, F. Rohmer, F. Pons, C. Franchet, S. Lecat, K. Y. C. Chow, R. Dagher, P. Gizzi, B. Didier, et al.
Small Neutralizing Molecules to Inhibit Actions of the Chemokine CXCL12
J. Biol. Chem., August 22, 2008; 283(34): 23189 - 23199.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. A. Trushin, A. Algeciras-Schimnich, S. R. Vlahakis, G. D. Bren, S. Warren, D. J. Schnepple, and A. D. Badley
Glycoprotein 120 Binding to CXCR4 Causes p38-Dependent Primary T Cell Death That Is Facilitated by, but Does Not Require Cell-Associated CD4
J. Immunol., April 15, 2007; 178(8): 4846 - 4853.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Melar, D. E. Ott, and T. J. Hope
Physiological Levels of Virion-Associated Human Immunodeficiency Virus Type 1 Envelope Induce Coreceptor-Dependent Calcium Flux
J. Virol., February 15, 2007; 81(4): 1773 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Chougnet and S. Gessani
Role of gp120 in dendritic cell dysfunction in HIV infection
J. Leukoc. Biol., November 1, 2006; 80(5): 994 - 1000.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. Delobel, M.-T. Nugeyre, M. Cazabat, K. Sandres-Saune, C. Pasquier, L. Cuzin, B. Marchou, P. Massip, R. Cheynier, F. Barre-Sinoussi, et al.
Naive T-cell depletion related to infection by x4 human immunodeficiency virus type 1 in poor immunological responders to highly active antiretroviral therapy.
J. Virol., October 1, 2006; 80(20): 10229 - 10236.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. G. Nguyen, K. C. Wolff, H. Yin, J. S. Caldwell, and K. L. Kuhen
"UnPAKing" Human Immunodeficiency Virus (HIV) Replication: Using Small Interfering RNA Screening To Identify Novel Cofactors and Elucidate the Role of Group I PAKs in HIV Infection
J. Virol., January 1, 2006; 80(1): 130 - 137.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Balabanian, B. Lagane, S. Infantino, K. Y. C. Chow, J. Harriague, B. Moepps, F. Arenzana-Seisdedos, M. Thelen, and F. Bachelerie
The Chemokine SDF-1/CXCL12 Binds to and Signals through the Orphan Receptor RDC1 in T Lymphocytes
J. Biol. Chem., October 21, 2005; 280(42): 35760 - 35766.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.