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Plasmacytoid Dendritic Cell Recruitment by Immobilized CXCR3 Ligands¹

Norbert Kohrgruber^*, Marion Gröger, Paul Meraner^*, Ernst Kriehuber^*,, Peter Petzelbauer, Sabine Brandt^*, Georg Stingl^*, Antal Rot and Dieter Maurer^2,*,

^* Division of Immunology, Allergy and Infectious Diseases and Division of General Dermatology, Department of Dermatology, Medical University of Vienna, Center of Molecular Medicine (CeMM) of the Austrian Academy of Sciences, and Novartis Institute for Biomedical Research, Vienna, Austria

Plasmacytoid dendritic cells (pDCs) recognize microbes, viruses in particular, and provide unique means of innate defense against them. The mechanism of pDC tissue recruitment remained enigmatic because the ligands of CXCR3, the cardinal chemokine receptor on pDCs, have failed to induce in vitro chemotaxis of pDCs in the absence of additional chemokines. In this study, we demonstrate that CXCR3 is sufficient to induce pDC migration, however, by a migratory mechanism that amalgamates the features of haptotaxis and chemorepulsion. To mediate "haptorepulsion" of pDCs, CXCR3 requires the encounter of its cognate ligands immobilized, optimally by heparan sulfate, in a form of a negative gradient. This is the first report of the absolute requirement of chemokine immobilization and presentation for its in vitro promigratory activity. The paradigmatic example of pDC haptorepulsion described here may represent a new pathophysiologically relevant migratory mechanism potentially used by other cells in response to other chemokines.

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