The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Estes, J. D.
Right arrow Articles by Burton, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Estes, J. D.
Right arrow Articles by Burton, G. F.
The Journal of Immunology, 2004, 173: 6169-6178.
Copyright © 2004 by The American Association of Immunologists

Follicular Dendritic Cell Regulation of CXCR4-Mediated Germinal Center CD4 T Cell Migration1

Jacob D. Estes*, Tyler C. Thacker*, Denise L. Hampton{ddagger}, Sariah A. Kell{dagger}, Brandon F. Keele*, Emily A. Palenske*, Kirk M. Druey{ddagger} and Gregory F. Burton2,{dagger}

Departments of * Microbiology and Molecular Biology and {dagger} Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602; and {ddagger} Molecular Signal Transduction Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852

Follicular dendritic cells (FDCs) up-regulate the chemokine receptor CXCR4 on CD4 T cells, and a major subpopulation of germinal center (GC) T cells (CD4+CD57+), which are adjacent to FDCs in vivo, expresses high levels of CXCR4. We therefore reasoned that GC T cells would actively migrate to stromal cell-derived factor-1 (CXCL12), the CXCR4 ligand, and tested this using Transwell migration assays with GC T cells and other CD4 T cells (CD57–) that expressed much lower levels of CXCR4. Unexpectedly, GC T cells were virtually nonresponsive to CXCL12, whereas CD57–CD4 T cells migrated efficiently despite reduced CXCR4 expression. In contrast, GC T cells efficiently migrated to B cell chemoattractant-1/CXCL13 and FDC supernatant, which contained CXCL13 produced by FDCs. Importantly, GC T cell nonresponsiveness to CXCL12 correlated with high ex vivo expression of regulator of G protein signaling (RGS), RGS13 and RGS16, mRNA and expression of protein in vivo. Furthermore, FDCs up-regulated both RGS13 and RGS16 mRNA expression in non-GC T cells, resulting in their impaired migration to CXCL12. Finally, GC T cells down-regulated RGS13 and RGS16 expression in the absence of FDCs and regained migratory competence to CXCL12. Although GC T cells express high levels of CXCR4, signaling through this receptor appears to be specifically inhibited by FDC-mediated expression of RGS13 and RGS16. Thus, FDCs appear to directly affect GC T cell migration within lymphoid follicles.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. Liang, G. Bansal, Z. Xie, and K. M. Druey
RGS16 Inhibits Breast Cancer Cell Growth by Mitigating Phosphatidylinositol 3-Kinase Signaling
J. Biol. Chem., August 7, 2009; 284(32): 21719 - 21727.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
Y. Wu, M. E. M. El Shikh, R. M. El Sayed, A. M. Best, A. K. Szakal, and J. G. Tew
IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation
Int. Immunol., June 1, 2009; 21(6): 745 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. S. Song, Y. H. Choi, J.-M. Kim, H. Lee, T.-J. Lee, and J.-H. Yoon
Suppression of prostaglandin E2-induced MUC5AC overproduction by RGS4 in the airway
Am J Physiol Lung Cell Mol Physiol, April 1, 2009; 296(4): L684 - L692.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. C. Thacker, X. Zhou, J. D. Estes, Y. Jiang, B. F. Keele, T. S. Elton, and G. F. Burton
Follicular Dendritic Cells and Human Immunodeficiency Virus Type 1 Transcription in CD4+ T Cells
J. Virol., January 1, 2009; 83(1): 150 - 158.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
J. M. Odegard, B. R. Marks, L. D. DiPlacido, A. C. Poholek, D. H. Kono, C. Dong, R. A. Flavell, and J. Craft
ICOS-dependent extrafollicular helper T cells elicit IgG production via IL-21 in systemic autoimmunity
J. Exp. Med., November 24, 2008; 205(12): 2873 - 2886.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Wu, S. Sukumar, M. E. El Shikh, A. M. Best, A. K. Szakal, and J. G. Tew
Immune Complex-Bearing Follicular Dendritic Cells Deliver a Late Antigenic Signal That Promotes Somatic Hypermutation
J. Immunol., January 1, 2008; 180(1): 281 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
K. Otto, H. Starz, J. C. Becker, and D. Schrama
Overexpression of Matrix Metalloproteinases, Chemokines, and Chemokine Receptors Relevant for Metastasis in Experimental Models Not an Indication of Lymph Node Metastases in Human Melanoma
Arch Dermatol, July 1, 2007; 143(7): 947 - 948.
[Full Text] [PDF]

Home page
 BloodHome page
M. Berthebaud, C. Riviere, P. Jarrier, A. Foudi, Y. Zhang, D. Compagno, A. Galy, W. Vainchenker, and F. Louache
RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes
Blood, November 1, 2005; 106(9): 2962 - 2968.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.