The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dave, S.
Right arrow Articles by McDaniel, L. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dave, S.
Right arrow Articles by McDaniel, L. S.
The Journal of Immunology, 2004, 173: 471-477.
Copyright © 2004 by The American Association of Immunologists

Dual Roles of PspC, a Surface Protein of Streptococcus pneumoniae, in Binding Human Secretory IgA and Factor H1

Sandhya Dave*, Stephanie Carmicle*, Sven Hammerschmidt2,§, Michael K. Pangburn and Larry S. McDaniel3,*,{dagger},{ddagger}

Departments of * Microbiology, {dagger} Surgery, and {ddagger} Medicine, University of Mississippi Medical Center, Jackson, MS 39216; § Department of Microbial Pathogenesis, German Research Centre for Biotechnology, Braunschweig, Germany; and Department of Biochemistry, University of Texas Health Science Center, Tyler, TX 75708

Streptococcus pneumoniae, also known as the pneumococcus, contains several surface proteins that along with the polysaccharide capsule function in antiphagocytic activities and evasion of the host immune system. These pneumococcal proteins interact with the host immune system in various ways and possess a wide range of biological activities that suggests that they may be involved at different stages of pneumococcal infection. PspC, also known as CbpA and SpsA, is one of several pneumococcal surface proteins that binds host proteins, including factor H (FH) and secretory IgA (sIgA) via the secretory component. Previous work by our laboratory has demonstrated that PspC on the surface of live pneumococcal cells binds FH. This paper provides evidence that FH activity is maintained in the presence of PspC and that the PspC binding site is located in the short consensus repeat 6–10 region of FH. We also report for the first time that although both FH and sIgA binding has been localized to the {alpha}-helical domain of PspC, the binding of FH to PspC is not inhibited by sIgA. ELISA, surface plasmon resonance, and flow cytometry indicate that the two host proteins do not compete for binding with PspC and likely do not share the same binding sites. We confirmed by Western analysis that the binding sites are separate using recombinant PspC proteins. These PspC variants bind FH yet fail to bind sIgA. Thus, we conclude that FH and sIgA can bind concurrently to the {alpha}-helical region of PspC.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Dieudonne-Vatran, S. Krentz, A. M. Blom, S. Meri, B. Henriques-Normark, K. Riesbeck, and B. Albiger
Clinical Isolates of Streptococcus pneumoniae Bind the Complement Inhibitor C4b-Binding Protein in a PspC Allele-Dependent Fashion
J. Immunol., June 15, 2009; 182(12): 7865 - 7877.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. V. McDowell, B. Huang, J. C. Fenno, and R. T. Marconi
Analysis of a Unique Interaction between the Complement Regulatory Protein Factor H and the Periodontal Pathogen Treponema denticola
Infect. Immun., April 1, 2009; 77(4): 1417 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
V. Sabharwal, S. Ram, M. Figueira, I. H. Park, and S. I. Pelton
Role of Complement in Host Defense against Pneumococcal Otitis Media
Infect. Immun., March 1, 2009; 77(3): 1121 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Lu, Z. Ma, T. S. Jokiranta, A. R. Whitney, F. R. DeLeo, and J.-R. Zhang
Species-Specific Interaction of Streptococcus pneumoniae with Human Complement Factor H
J. Immunol., November 15, 2008; 181(10): 7138 - 7146.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. R. Quin, Q. C. Moore III, J. A. Thornton, and L. S. McDaniel
Peritoneal Challenge Modulates Expression of Pneumococcal Surface Protein C during Bacteremia in Mice
Infect. Immun., March 1, 2008; 76(3): 1122 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. R. Quin, C. Onwubiko, Q. C. Moore, M. F. Mills, L. S. McDaniel, and S. Carmicle
Factor H Binding to PspC of Streptococcus pneumoniae Increases Adherence to Human Cell Lines In Vitro and Enhances Invasion of Mouse Lungs In Vivo
Infect. Immun., August 1, 2007; 75(8): 4082 - 4087.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Hammerschmidt, V. Agarwal, A. Kunert, S. Haelbich, C. Skerka, and P. F. Zipfel
The Host Immune Regulator Factor H Interacts via Two Contact Sites with the PspC Protein of Streptococcus pneumoniae and Mediates Adhesion to Host Epithelial Cells
J. Immunol., May 1, 2007; 178(9): 5848 - 5858.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. R. Quin, Q. C. Moore III, and L. S. McDaniel
Pneumolysin, PspA, and PspC Contribute to Pneumococcal Evasion of Early Innate Immune Responses during Bacteremia in Mice
Infect. Immun., April 1, 2007; 75(4): 2067 - 2070.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. D. Ogunniyi, K. S. LeMessurier, R. M. A. Graham, J. M. Watt, D. E. Briles, U. H. Stroeher, and J. C. Paton
Contributions of Pneumolysin, Pneumococcal Surface Protein A (PspA), and PspC to Pathogenicity of Streptococcus pneumoniae D39 in a Mouse Model
Infect. Immun., April 1, 2007; 75(4): 1843 - 1851.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. V. McDowell, J. Frederick, L. Stamm, and R. T. Marconi
Identification of the Gene Encoding the FhbB Protein of Treponema denticola, a Highly Unique Factor H-Like Protein 1 Binding Protein
Infect. Immun., February 1, 2007; 75(2): 1050 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. M. A. Graham and J. C. Paton
Differential Role of CbpA and PspA in Modulation of In Vitro CXC Chemokine Responses of Respiratory Epithelial Cells to Infection with Streptococcus pneumoniae
Infect. Immun., December 1, 2006; 74(12): 6739 - 6749.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. R. Kerr, G. K. Paterson, J. McCluskey, F. Iannelli, M. R. Oggioni, G. Pozzi, and T. J. Mitchell
The Contribution of PspC to Pneumococcal Virulence Varies between Strains and Is Accomplished by Both Complement Evasion and Complement-Independent Mechanisms
Infect. Immun., September 1, 2006; 74(9): 5319 - 5324.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. M. Hovis, M. E. Schriefer, S. Bahlani, and R. T. Marconi
Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever.
Infect. Immun., August 1, 2006; 74(8): 4519 - 4529.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Lu, Y. Ma, and J.-R. Zhang
Streptococcus pneumoniae Recruits Complement Factor H through the Amino Terminus of CbpA
J. Biol. Chem., June 2, 2006; 281(22): 15464 - 15474.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. Kolberg, A. Aase, S. Bergmann, T. K. Herstad, G. Rodal, R. Frank, M. Rohde, and S. Hammerschmidt
Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface.
Microbiology, May 1, 2006; 152(Pt 5): 1307 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. M. Hovis, J. P. Jones, T. Sadlon, G. Raval, D. L. Gordon, and R. T. Marconi
Molecular Analyses of the Interaction of Borrelia hermsii FhbA with the Complement Regulatory Proteins Factor H and Factor H-Like Protein 1
Infect. Immun., April 1, 2006; 74(4): 2007 - 2014.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
N. Nagano, Y. Nagano, R. Nakano, R. Okamoto, and M. Inoue
Genetic diversity of the C protein beta-antigen gene and its upstream regions within clonally related groups of type Ia and Ib group B streptococci.
Microbiology, March 1, 2006; 152(Pt 3): 771 - 778.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. Bergmann and S. Hammerschmidt
Versatility of pneumococcal surface proteins
Microbiology, February 1, 2006; 152(2): 295 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. V. McDowell, J. Lankford, L. Stamm, T. Sadlon, D. L. Gordon, and R. T. Marconi
Demonstration of Factor H-Like Protein 1 Binding to Treponema denticola, a Pathogen Associated with Periodontal Disease in Humans
Infect. Immun., November 1, 2005; 73(11): 7126 - 7132.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. E. Briles, L. Novak, M. Hotomi, F. W. van Ginkel, and J. King
Nasal Colonization with Streptococcus pneumoniae Includes Subpopulations of Surface and Invasive Pneumococci
Infect. Immun., October 1, 2005; 73(10): 6945 - 6951.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. R. Kerr, G. K. Paterson, A. Riboldi-Tunnicliffe, and T. J. Mitchell
Innate Immune Defense against Pneumococcal Pneumonia Requires Pulmonary Complement Component C3
Infect. Immun., July 1, 2005; 73(7): 4245 - 4252.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. V. McDowell, M. E. Harlin, E. A. Rogers, and R. T. Marconi
Putative Coiled-Coil Structural Elements of the BBA68 Protein of Lyme Disease Spirochetes Are Required for Formation of Its Factor H Binding Site
J. Bacteriol., February 15, 2005; 187(4): 1317 - 1323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2004 by The American Association of Immunologists, Inc. All rights reserved.