HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Krotkova, A. Articles by Eichmann, K. Search for Related Content PubMed PubMed Citation Articles by Krotkova, A. Articles by Eichmann, K.

Delayed and Restricted Expression Limits Putative Instructional Opportunities of V1.1/V2 TCR in / Lineage Choice in the Thymus

Max-Planck-Institut für Immunbiologie, Freiburg, Germany

Development of and T cells depends on productive rearrangement of the appropriate TCR genes and their subsequent expression as proteins. TCR and TCR proteins first appear in DN3 and DN4 thymocytes, respectively. So far, it is not clear whether this is due to a delayed expression of TCR proteins or to a more rapid progression to DN4 of thymocytes expressing TCR. The answer to this question bears on the distinction between instructive and stochastic models of / lineage decision. To study this question, we first monitored initial TCR protein expression in wild-type and TCR transgenic mice in reaggregate thymic organ cultures. A TCR transgene was expressed in nearly all DN3 and DN4 cells, accelerated DN3 to DN4 transition, and strongly diminished the number of cells that express TCR proteins. In contrast, TCR transgenes were expressed only in a fraction of DN4 cells, did not accelerate DN3 to DN4 transition, and did not reduce the number of DN4 cells expressing TCR proteins. The TCR transgene partially inhibited endogenous TCR rearrangements, whereas the TCR transgenes did not inhibit endogenous TCR rearrangements. Second, we analyzed frequencies of productive TCR and TCR V(D)J junctions in DN3 and DN4 subsets. Most importantly, frequencies of productive TCR rearrangements (V5, V1.1, and V2) appeared unselected in DN3. The results suggest a late and restricted expression of the corresponding TCR, severely limiting their putative instructional opportunities in / divergence.

This article has been cited by other articles:

				T. Kreslavsky, A. I. Garbe, A. Krueger, and H. von Boehmer T cell receptor-instructed {alpha}{beta} versus {gamma}{delta} lineage commitment revealed by single-cell analysis J. Exp. Med., May 12, 2008; 205(5): 1173 - 1186. [Abstract] [Full Text] [PDF]

				C. E. Busse, A. Krotkova, and K. Eichmann The TCR{beta} Enhancer Is Dispensable for the Expression of Rearranged TCR{beta} Genes in Thymic DN2/DN3 Populations but Not at Later Stages J. Immunol., September 1, 2005; 175(5): 3067 - 3074. [Abstract] [Full Text] [PDF]