HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maffia, P. Articles by Garside, P. Search for Related Content PubMed PubMed Citation Articles by Maffia, P. Articles by Garside, P. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Dietary Proteins Joint Disorders

Inducing Experimental Arthritis and Breaking Self-Tolerance to Joint-Specific Antigens with Trackable, Ovalbumin-Specific T Cells¹

Pasquale Maffia^*,, James M. Brewer^*, J. Alastair Gracie, Angela Ianaro, Bernard P. Leung^*, Paul J. Mitchell^*, Karen M. Smith^*, Iain B. McInnes and Paul Garside^2,*

^* Division of Immunology, Infection, and Inflammation, University of Glasgow, Western Infirmary, Glasgow, United Kingdom; Centre for Rheumatic Diseases, Division of Immunology, Infection and Inflammation, Glasgow Royal Infirmary, Glasgow, United Kingdom; and Department of Experimental Pharmacology, University of Naples, Napoli, Italy

The importance of T cell Ag specificity and Th1 vs Th2 phenotype in synovial inflammation remains controversial. Using OVA-specific TCR transgenic T cells from DO11.10 mice, we demonstrate that mice receiving Th1, but not Th2, cells display a transient arthritis following immunization that is characterized by synovial hyperplasia, cellular infiltration, and cartilage erosion. OVA-specific T cells also accumulated in inflamed joints, suggesting that they could exert their inflammatory effect locally in the joint or in the draining lymph node. Importantly, this pathology was accompanied by a breakdown in self-tolerance, as evidenced by the induction of collagen-specific T and B cell responses. This model directly demonstrates a pivotal role for Th1 cells of an irrelevant specificity in the development of inflammatory arthritis. Furthermore, the ability to track these cells in vivo will make feasible studies revealing the dynamic role of T cells in arthritis.

This article has been cited by other articles:

				M B Nickdel, P Conigliaro, G Valesini, S Hutchison, R Benson, R V Bundick, A J Leishman, I B McInnes, J M Brewer, and P Garside Dissecting the contribution of innate and antigen-specific pathways to the breach of self-tolerance observed in a murine model of arthritis Ann Rheum Dis, June 1, 2009; 68(6): 1059 - 1066. [Abstract] [Full Text] [PDF]

				S. L. Jongbloed, R. A. Benson, M. B. Nickdel, P. Garside, I. B. McInnes, and J. M. Brewer Plasmacytoid Dendritic Cells Regulate Breach of Self-Tolerance in Autoimmune Arthritis J. Immunol., January 15, 2009; 182(2): 963 - 968. [Abstract] [Full Text] [PDF]

				U. Niesner, I. Albrecht, M. Janke, C. Doebis, C. Loddenkemper, M. H. Lexberg, K. Eulenburg, S. Kreher, J. Koeck, R. Baumgrass, et al. Autoregulation of Th1-mediated inflammation by twist1 J. Exp. Med., August 4, 2008; 205(8): 1889 - 1901. [Abstract] [Full Text] [PDF]

				T. Guichelaar, C. B. ten Brink, P. J. van Kooten, S. E. Berlo, C. P. Broeren, W. van Eden, and F. Broere Autoantigen-Specific IL-10-Transduced T Cells Suppress Chronic Arthritis by Promoting the Endogenous Regulatory IL-10 Response J. Immunol., February 1, 2008; 180(3): 1373 - 1381. [Abstract] [Full Text] [PDF]

				P. Maffia, G. Grassia, P. Di Meglio, R. Carnuccio, L. Berrino, P. Garside, A. Ianaro, and A. Ialenti Neutralization of Interleukin-18 Inhibits Neointimal Formation in a Rat Model of Vascular Injury Circulation, August 1, 2006; 114(5): 430 - 437. [Abstract] [Full Text] [PDF]