HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Camelo, S. Articles by McMenamin, P. G. Search for Related Content PubMed PubMed Citation Articles by Camelo, S. Articles by McMenamin, P. G. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB C.I. PIGMENT BLUE 1

The Distribution of Antigen in Lymphoid Tissues following Its Injection into the Anterior Chamber of the Rat Eye¹

School of Anatomy and Human Biology, University of Western Australia, Crawley, Perth, Western Australia

Injection of Ag into the anterior chamber (AC) of the eye induces deviant immune responses. It has been proposed that Ag internalized by ocular APCs is presented in a tolerogenic fashion in the spleen. However, the nature and distribution of the Ag-bearing cells in the lymphoid organs remain unclear. Fluorescent-labeled Ag (dextran, BSA) injected into the AC of Lewis rats was detected in the subcapsular sinus of the right submandibular lymph nodes (LNs) and cervical LNs, the marginal zone of the spleen, and the medulla of the mesenteric LNs. In the spleen, Ag-bearing cells were CD1⁺, CD11b⁺, ED1⁺, ED2^low, ED3⁺, CD86^low, OX6⁺, CD11c^–, ED5^– and in the LNs were CD4⁺, CD8⁺, CD80⁺, and OX41⁺ suggesting these were lymphoid organ resident macrophages. These Ag-bearing macrophages were located adjacent to CD4⁺ cells, CD8⁺ cells, and NK cells in the LNs and spleen and to marginal zone B cells in the spleen. No interaction with T cells was observed. The data demonstrates that Ag derived from the AC of the eye is mainly internalized by resident macrophages in the LNs and spleen which are ideally placed to interact with cells involved in the induction of deviant ocular immune responses. The extensive distribution of Ag in LNs draining the upper airway and gastrointestinal tracts, together with the phenotype of Ag-bearing cells in the lymphoid organs, suggests that Ag leaves the eye predominantly in a soluble form and implies other mechanisms of tolerance may contribute to ocular-specific immune responses.

This article has been cited by other articles:

				H. R. Chinnery, E. Pearlman, and P. G. McMenamin Cutting Edge: Membrane Nanotubes In Vivo: A Feature of MHC Class II+ Cells in the Mouse Cornea J. Immunol., May 1, 2008; 180(9): 5779 - 5783. [Abstract] [Full Text] [PDF]

				L. Kuffova, M. Netukova, L. Duncan, A. Porter, B. Stockinger, and J. V. Forrester Cross Presentation of Antigen on MHC Class II via the Draining Lymph Node after Corneal Transplantation in Mice J. Immunol., February 1, 2008; 180(3): 1353 - 1361. [Abstract] [Full Text] [PDF]

				L. A. Nichols, Y. Chen, T. A. Colella, C. L. Bennett, B. E. Clausen, and V. H. Engelhard Deletional Self-Tolerance to a Melanocyte/Melanoma Antigen Derived from Tyrosinase Is Mediated by a Radio-Resistant Cell in Peripheral and Mesenteric Lymph Nodes J. Immunol., July 15, 2007; 179(2): 993 - 1003. [Abstract] [Full Text] [PDF]

				L. Lajavardi, A. Bochot, S. Camelo, B. Goldenberg, M.-C. Naud, F. Behar-Cohen, E. Fattal, and Y. de Kozak Downregulation of Endotoxin-Induced Uveitis by Intravitreal Injection of Vasoactive Intestinal Peptide Encapsulated in Liposomes Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3230 - 3238. [Abstract] [Full Text] [PDF]

				H. Xu, M. Chen, D. M. Reid, and J. V. Forrester LYVE-1-Positive Macrophages Are Present in Normal Murine Eyes Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2162 - 2171. [Abstract] [Full Text] [PDF]

				H. Xu, R. Dawson, J. V. Forrester, and J. Liversidge Identification of Novel Dendritic Cell Populations in Normal Mouse Retina Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1701 - 1710. [Abstract] [Full Text] [PDF]

				C. Terrada, S. Fisson, Y. De Kozak, M. Kaddouri, P. Lehoang, D. Klatzmann, B. L. Salomon, and B. Bodaghi Regulatory T cells control uveoretinitis induced by pathogenic Th1 cells reacting to a specific retinal neoantigen. J. Immunol., June 15, 2006; 176(12): 7171 - 7179. [Abstract] [Full Text] [PDF]

				S. Camelo, J. Kezic, A. Shanley, P. Rigby, and P. G. McMenamin Antigen from the Anterior Chamber of the Eye Travels in a Soluble Form to Secondary Lymphoid Organs via Lymphatic and Vascular Routes. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1039 - 1046. [Abstract] [Full Text] [PDF]

				O. M. Z. Howard, H. F. Dong, S. B. Su, R. R. Caspi, X. Chen, P. Plotz, and J. J. Oppenheim Autoantigens signal through chemokine receptors: uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate Blood, June 1, 2005; 105(11): 4207 - 4214. [Abstract] [Full Text] [PDF]

				P. A. Dullforce, K. L. Garman, G. W. Seitz, R. J. Fleischmann, S. M. Crespo, S. R. Planck, D. C. Parker, and J. T. Rosenbaum APCs in the Anterior Uveal Tract Do Not Migrate to Draining Lymph Nodes J. Immunol., June 1, 2004; 172(11): 6701 - 6708. [Abstract] [Full Text] [PDF]