| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Sterile Transcription during Ig Chain Gene Rearrangement1
* Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan; and
Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
Pax-5 is the key regulator in B cell development. Pax-5-deficient mice show defects in B cell commitment and recombination of IgH chain gene rearrangement from DJ to VDJ. Previously, we found that Pax-5 bound to KI and KII sites, which play a crucial role in 
-chain gene rearrangement. However, the function of Pax-5 in Ig chain gene rearrangement has not been investigated. To address this issue, we newly established pre-BI cell lines expressing the pre-B cell receptor from Pax-5-deficient mice and used them in an in vitro culture system, in which -chain gene rearrangement is induced by removing IL-7. By examining the Pax-5-deficient pre-BI (knockout (KO)) cells, we show in this study that, despite recombination-activating gene 1 and 2 expression, these KO cells did not rearrange the -chain gene following the absence of sterile transcription. Consistent with these data, fluorescent in situ hybridization analyses revealed that the J locus in KO cells was located at the nuclear periphery as a repressive compartment. Transfection of KO cells with Pax-5 constructs indicated that the transactivation domain of Pax-5 was required for sterile transcription and -chain gene rearrangement. Moreover, the hormone-inducible system in KO cells demonstrated that Pax-5 directly functioned in sterile transcription. These results indicate that Pax-5 is necessary for sterile transcription during Ig chain gene rearrangement.
This article has been cited by other articles:
|
|
 |
|
  Z. Liu, Z. Ma, L. S. Terada, and W. T. Garrard Divergent Roles of RelA and c-Rel in Establishing Chromosomal Loops upon Activation of the Ig{kappa} Gene J. Immunol., September 15, 2009; 183(6): 3819 - 3830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Schneider, M. A. Manzan, R. B. Crawford, W. Chen, and N. E. Kaminski 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Mediated Impairment of B Cell Differentiation Involves Dysregulation of Paired Box 5 (Pax5) Isoform, Pax5a J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 463 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Fitzsimmons, R. M. Bernstein, E. E. Max, J. A. Skok, and M. A. Shapiro Dynamic Changes in Accessibility, Nuclear Positioning, Recombination, and Transcription at the Ig{kappa} Locus J. Immunol., October 15, 2007; 179(8): 5264 - 5273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. van Zelm, M. van der Burg, D. de Ridder, B. H. Barendregt, E. F. E. de Haas, M. J. T. Reinders, A. C. Lankester, T. Revesz, F. J. T. Staal, and J. J. M. van Dongen Ig Gene Rearrangement Steps Are Initiated in Early Human Precursor B Cell Subsets and Correlate with Specific Transcription Factor Expression J. Immunol., November 1, 2005; 175(9): 5912 - 5922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bai, L. Srinivasan, L. Perkins, and M. L. Atchison Protein Acetylation Regulates Both PU.1 Transactivation and Ig{kappa} 3' Enhancer Activity J. Immunol., October 15, 2005; 175(8): 5160 - 5169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. McDevit, L. Perkins, M. L. Atchison, and B. S. Nikolajczyk The Ig{kappa}3' Enhancer Is Activated by Gradients of Chromatin Accessibility and Protein Association J. Immunol., March 1, 2005; 174(5): 2834 - 2842. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
